Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Michel, Desautels"'
Publikováno v:
PLoS ONE, Vol 4, Iss 7, p e6179 (2009)
BACKGROUND:Schizosaccharomyces pombe pik1 encodes a phosphatidylinositol 4-kinase, reported to bind Cdc4, but not Cdc4(G107S). PRINCIPAL FINDINGS:Gene deletion revealed that pik1 is essential. In cells with pik1 deleted, ectopic expression of a loss-
Externí odkaz:
https://doaj.org/article/92742f4520f744458668fa2d49ccbce5
Autor:
Michelle A, Alting-Mees, Eddy P, Risseeuw, Enwu, Liu, Michel, Desautels, William A, Crosby, Sean M, Hemmingsen
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 313
Intracellular localization is important for the characterization of a gene product. Microscopy of fluorescent protein fusions has become the method of choice to define the spatial and temporal behavior of a protein. We show here that recombinant anti
Autor:
Enwu Liu, Michel Desautels, Eddy Risseeuw, Michelle A. Alting-Mees, William A. Crosby, Sean M. Hemmingsen
Intracellular localization is important for the characterization of a gene product. Microscopy of fluorescent protein fusions has become the method of choice to define the spatial and temporal behavior of a protein. We show here that recombinant anti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5267d04366340070e54283113454594d
https://doi.org/10.1385/1-59259-958-3:097
https://doi.org/10.1385/1-59259-958-3:097
Autor:
Sean M. Hemmingsen, Eric Escobar-Cabrera, Lawrence P. McIntosh, Michel Desautels, Meenakshi Venkatesan
Cytokinesis is the process by which one cell divides into two. Key in the cytokinetic mechanism of Schizosaccharomyces pombe is the contractile ring myosin, which consists of two heavy chains (Myo2p), two essential light chains (Cdc4p), and two regul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bcb364f10f44f9dc873cbd36eee09261
https://doi.org/10.1021/bi050641c
https://doi.org/10.1021/bi050641c
Autor:
Sean M. Hemmingsen, Carolyn M. Slupsky, Jason P. Den Haese, Lawrence P. McIntosh, Michel Desautels
Publikováno v:
The Journal of biological chemistry. 276(8)
The proposed function of Cdc4p, an essential contractile ring protein in Schizosaccharomyces pombe, is that of a myosin essential light chain. However, five conditionally lethal cdc4 alleles exhibit complementation in diploids. Such interallelic comp
Autor:
Terry Huebert, Lawrence P. McIntosh, Michel Desautels, Sean M. Hemmingsen, Ruohong Zhao, Carolyn M. Slupsky
Publikováno v:
The Journal of biological chemistry. 276(8)
The Schizosaccharomyces pombe Cdc4 protein is required for the formation and function of the contractile ring, presumably acting as a myosin light chain. By using NMR spectroscopy, we demonstrate that purified Cdc4p is a monomeric protein with two st
Autor:
Jean Himms-Hagen, Michel Desautels
Publikováno v:
Canadian Journal of Biochemistry. 59:619-625
Growth of brown adipose tissue in cold-acclimated rats, known to result in a large increase in capacity for noradrenaline-induced thermogenesis, is accompanied by changes in mitochondria (increase in concentration of binding sites for GDP with no cha
Publikováno v:
Biochemistry and Cell Biology. 65:955-959
The time course of changes in the level of uncoupling protein mRNA when cold-acclimated mice were returned to a thermoneutral environment (33 °C) was examined using a cDNA probe. Upon deacclimation, there was a marked loss of uncoupling protein mRNA
Autor:
Alfred L. Goldberg, Michel Desautels
Publikováno v:
Journal of Biological Chemistry. 257:11673-11679
Autor:
Alfred L. Goldberg, Michel Desautels
Publikováno v:
Proceedings of the National Academy of Sciences. 79:1869-1873
A large fraction (30-50%) of the various proteins synthesized within isolated rat liver mitochondria were degraded to amino acids within 60 min after synthesis. Incomplete mitochondrial polypeptides resulting from the incorporation of puromycin were