Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Michaela Vlasakova"'
Autor:
Slavomira Salamunova, Anna Jackova, Rene Mandelik, Jaroslav Novotny, Michaela Vlasakova, Stefan Vilcek
Publikováno v:
BMC Veterinary Research, Vol 14, Iss 1, Pp 1-9 (2018)
Abstract Background Surveillance and characterization of pig enteric viruses such as transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), rotavirus, astrovirus (PAstV), sapovirus (PSaV), kobuvirus and other agents is es
Externí odkaz:
https://doaj.org/article/38a5caa56b8043f087f202bfb1b91d9f
Autor:
Rene Mandelik, Anna Jackova, Ivan Sliz, Stefan Vilcek, Mariana Kolesarova, Slavomira Salamunova, Jaroslav Novotny, Michaela Vlasakova
Publikováno v:
Infection, Genetics and Evolution. 49:73-77
The porcine kobuvirus 1 (PKV-1) is believed to be an enteric virus. To investigate the prevalence of PKV-1 in pigs, virus was detected by RT-PCR in rectal swabs originating from 414 healthy and diarrheic pigs of different age categories on farms in S
Publikováno v:
Acta virologica. 61(2)
Bovine coronavirus (BCoV) is considered an important pathogen in cattle worldwide. It is a causative agent of enteric and respiratory diseases of cattle. The S1 subunit of the viral S glycoprotein is responsible for virus binding to host-cell recepto
Publikováno v:
Acta virologica. 57:17-25
In this study, a major part of genome of the pestivirus isolate 297 from Slovakia, comprising the 7195 nt-long 5΄-UTR-NS3 region was sequenced and analyzed. Conserved cleavage sites between individual viral proteins of this region were determined an
Publikováno v:
Journal of Virological Methods. 165:216-221
Light Upon eXtension real-time PCR (LUX real-time PCR) assay was developed for the detection of porcine circovirus type 2 (PCV2). The primers flanking a 114 bp fragment were selected from ORF1. The optimized assay could detect 20 viral copies of pBlu
Publikováno v:
Journal of wildlife diseases. 51(3)
As the number of free-living wild boars (Sus scrofa L.) continues to rise in Slovakia, the probability of pathogen transmission between susceptible species increases. We investigated the distribution and genetic characterization of porcine parvovirus
Publikováno v:
Berliner und Munchener tierarztliche Wochenschrift. 128(1-2)
PRRSV was confirmed by a nested RT-PCR in two out of 129 samples originating from wild boars from Eastern Slovakia. Virus isolates were genetically typed in ORF7 as PRRSV-1, EU-1 with 99.7% (sample 14WB) and 100% (sample 10WB) nucleotide sequence sim
Publikováno v:
Virus genes. 45(3)
All twenty-nine PRRSV ORF7 nucleotide sequences obtained from clinical samples originating from the three Central European countries Austria (n = 7), Czech Republic (n = 12), and Slovakia (n = 10) belonged to type 1, subtype I (EU-1). Twenty-seven se
Publikováno v:
Journal of virological methods. 179(2)
A novel, real-time PCR system for the detection of porcine circovirus type 2 (PCV2) was developed. The system employed Plexor technology and detected 10(8)-10(1) copies per reaction of PCV2 DNA within a recombinant plasmid. The examination of clinica
Publikováno v:
Research in veterinary science. 90(1)
Of 120 clinical specimens obtained from pigs bred on 28 PMWS-affected farms in Slovakia, porcine circovirus type 2 (PCV-2) was detected by single PCR in 77 samples. A short 224 bp fragment of ORF2 was used for preliminary grouping of isolates by phyl