Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducible promoter P pst

Autor: Kyra Hoffmann, Birthe Halmschlag, Simon Briel, Michaela Sieben, Sastia Putri, Eiichiro Fukusaki, Lars M. Blank, Jochen Büchs

Publikováno v: Biotechnology Progress. 39

Poly-γ-glutamic acid (γ-PGA) is a biopolymer with a wide range of applications, mainly produced using Bacillus strains. The formation and concomitant secretion of γ-PGA increases the culture broth viscosity, while enzymatic depolymerisation and de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae8d2e2d2b869397c96f321cbb756062
https://doi.org/10.1002/btpr.3293

Contact-free determination of viscosity in multiple parallel samples

Autor: Michaela Sieben, Jochen Büchs, René Hanke

Publikováno v: Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
Scientific Reports
Scientific reports 9, 8335 (2019). doi:10.1038/s41598-019-44859-z

Scientific reports 9(1), 8335 (2019). doi:10.1038/s41598-019-44859-z
Published by Macmillan Publishers Limited, part of Springer Nature, [London]

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74fea61411078407b55c0cba87f14f3c
http://link.springer.com/article/10.1038/s41598-019-44859-z

Reassessing the out-of-phase phenomenon in shake flasks by evaluating the angle-dependent liquid distribution relative to the direction of the centrifugal acceleration

Autor: Michaela Sieben, Amizon Azizan, Georg Wandrey, Jochen Büchs

Publikováno v: Biotechnology and bioengineering. 116(11)

Shake flasks are still the most relevant experimental tool in the development of viscous fermentation processes. The phase number, which defines the onset of the unfavorable out-of-phase (OP) phenomenon in shake flasks, was previously defined via spe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f56e6e61e7b9faa2740b0b83728a9fc7
https://pubmed.ncbi.nlm.nih.gov/31350917

Improvement and scale-down of a Trichoderma reesei shake flask protocol to microtiter plates enables high-throughput screening

Autor: Jochen Büchs, Elena Antonov, Ronald Hommes, Heiner Giese, Kristina Meier, Paulien Kruithof, Michaela Sieben

Publikováno v: Journal of Bioscience and Bioengineering. 118:702-709

Nowadays, high-throughput screening is essential for determining the best microbial strains and fermentation conditions. Although microtiter plates allow higher throughput in screening than shake flasks, they do not guarantee sufficient oxygen supply

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2791f8a6a82d704979c24959f01e49a
https://doi.org/10.1016/j.jbiosc.2014.05.016

Testing plasmid stability of Escherichia coli using the Continuously Operated Shaken BIOreactor System

Autor: Simone Fuchs, Laura Ann Chin, Gregor Steinhorn, Jochen Büchs, Lars Regestein, Michaela Sieben, Carsten H. G. Müller

Publikováno v: Biotechnology progress. 32(6)

Plasmids are common vectors to genetically manipulate Escherichia coli or other microorganisms. They are easy to use and considerable experience has accumulated on their application in heterologous protein production. However, plasmids can be lost du

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a796fbaa9e6da23c26fb6a4c63d12da6
https://pubmed.ncbi.nlm.nih.gov/27593226