Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells

Autor: Sarah Lensch, Michael H Herschl, Connor H Ludwig, Joydeb Sinha, Michaela M Hinks, Adi Mukund, Taihei Fujimori, Lacramioara Bintu

Publikováno v: eLife, Vol 11 (2022)

In mammalian cells genes that are in close proximity can be transcriptionally coupled: silencing or activating one gene can affect its neighbors. Understanding these dynamics is important for natural processes, such as heterochromatin spreading durin

Externí odkaz: https://doaj.org/article/10f76403709a4bd3b4ad4fbed615dccd

Systematic discovery of recombinases for efficient integration of large DNA sequences into the human genome.

Autor: Durrant MG; Arc Institute, Palo Alto, CA, USA.; Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.; Department of Genetics, Stanford University, Stanford, CA, USA., Fanton A; Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.; University of California, Berkeley-University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA, USA., Tycko J; Department of Genetics, Stanford University, Stanford, CA, USA., Hinks M; Department of Bioengineering, Stanford University, Stanford, CA, USA., Chandrasekaran SS; Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.; University of California, Berkeley-University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA, USA., Perry NT; Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.; University of California, Berkeley-University of California, San Francisco Graduate Program in Bioengineering, Berkeley, CA, USA., Schaepe J; Department of Bioengineering, Stanford University, Stanford, CA, USA., Du PP; Department of Genetics, Stanford University, Stanford, CA, USA.; Cancer Biology Program, Stanford University, Stanford, CA, USA., Lotfy P; Laboratory of Molecular and Cell Biology, Salk Institute for Biological Studies, La Jolla, CA, USA., Bassik MC; Department of Genetics, Stanford University, Stanford, CA, USA. bassik@stanford.edu., Bintu L; Department of Bioengineering, Stanford University, Stanford, CA, USA. lbintu@stanford.edu., Bhatt AS; Department of Genetics, Stanford University, Stanford, CA, USA. asbhatt@stanford.edu.; Department of Medicine (Hematology), Stanford University, Stanford, CA, USA. asbhatt@stanford.edu., Hsu PD; Arc Institute, Palo Alto, CA, USA. pdhsu@berkeley.edu.; Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA. pdhsu@berkeley.edu.; Laboratory of Molecular and Cell Biology, Salk Institute for Biological Studies, La Jolla, CA, USA. pdhsu@berkeley.edu.; Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA, USA. pdhsu@berkeley.edu.; Center for Computational Biology, University of California, Berkeley, Berkeley, CA, USA. pdhsu@berkeley.edu.

Publikováno v: Nature biotechnology [Nat Biotechnol] 2023 Apr; Vol. 41 (4), pp. 488-499. Date of Electronic Publication: 2022 Oct 10.

Massively parallel RNA device engineering in mammalian cells with RNA-Seq.

Autor: Xiang JS; Department of Bioengineering, 443 Via Ortega, MC 4245, Stanford University, Stanford, CA, 94305, USA., Kaplan M; Department of Bioengineering, 443 Via Ortega, MC 4245, Stanford University, Stanford, CA, 94305, USA., Dykstra P; Department of Bioengineering, 443 Via Ortega, MC 4245, Stanford University, Stanford, CA, 94305, USA., Hinks M; Department of Bioengineering, 443 Via Ortega, MC 4245, Stanford University, Stanford, CA, 94305, USA., McKeague M; Department of Pharmacology and Therapeutics, McGill University, 3655 Prom. Sir-William-Osler, Montreal, Quebec, H3G 1Y6, Canada.; Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, Quebec, H3A 0B8, Canada., Smolke CD; Department of Bioengineering, 443 Via Ortega, MC 4245, Stanford University, Stanford, CA, 94305, USA. csmolke@stanford.edu.; Chan Zuckerberg Biohub, San Francisco, CA, 94158, USA. csmolke@stanford.edu.

Publikováno v: Nature communications [Nat Commun] 2019 Sep 23; Vol. 10 (1), pp. 4327. Date of Electronic Publication: 2019 Sep 23.