Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Michaela Kreiner"'
Autor:
Michaela Kreiner, Carole C. Perry, Siddharth V. Patwardhan, Sharon M. Kelly, Stephen A. Holt, Christopher F. van der Walle
Publikováno v:
Biomacromolecules. 11:3126-3135
Silicatein alpha exists within the protein filament of silica spicules of the marine sponge Tethya aurantium in a predominantly beta-sheet structure. However, it is produced in a soluble form with mixed alpha-helix/beta-sheet structure akin to its ca
Autor:
Clive S. McKimmie, Christopher F. van der Walle, Michaela Kreiner, Stephen A. Holt, Mark D. Singh, Gerard J. Graham
Publikováno v:
Biochemical and Biophysical Research Communications. 390:716-721
We present the first report utilizing, and showing the functional relevance of, self-assembling polyvalent ligands specific for integrin alpha5beta1 in murine embryonic stem (mES) cell adhesion. Di, tri and tetrameric 9th-10th type III fibronectin do
Autor:
James Beattie, Zongyi Li, Helen J. Mardon, C.F. van der Walle, Michaela Kreiner, Sharon M. Kelly
Publikováno v:
Protein Engineering Design and Selection. 21:553-560
Substrates utilising clustered arginine-glycine-aspartic acid (RGD) ligand displays support greater cell adhesion over random displays. However, cell adhesion to integrin 5β1 requires the synergy site on the 9th type III fibronectin domain (FIII) in
Publikováno v:
Biotechnology and Bioengineering. 95:767-771
We have developed an electrospray ionisation mass spectrometry (ESI-MS) technique that can be applied to rapidly determine the number of intact active sites in proteins. The methodology relies on inhibiting the protein with an active-site irreversibl
Autor:
Marie-Claire Parker, Michaela Kreiner
Publikováno v:
Biotechnology Letters. 27:1571-1577
Protein-coated microcrystals (PCMC), a biocatalyst preparation previously demonstrated to display substantially increased transesterification activity of proteases and lipases in organic solvents when compared to their as received counterparts [Krein
Autor:
Michaela Kreiner, Norah O'Farrell, João F. Amorim Fernandes, Marie-Claire Parker, Peter J. Halling
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 33:65-72
Previously we reported a new high activity biocatalyst for use in organic media, termed protein-coated microcrystals (PCMC) [M. Kreiner, B.D. Moore, M.C. Parker, Chem. Commun. 12 (2001) 1906]. These novel biocomposites consist of water-soluble micron
Publikováno v:
Enzyme and Microbial Technology. 30:346-353
A recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen egg white lysozyme (HEWL), was investigated with regard to its susceptibility to oxidative stress. The culture response to oxidative stress, produced eithe
Publikováno v:
Biotechnology and Bioengineering. 70:662-669
A recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen egg white lysozyme, was investigated with regard to its susceptibility to "oxidative stress" in submerged culture in bioreactor systems. The culture respon
Publikováno v:
Biochemical and biophysical research communications. 407(4)
Progress towards endometrial tissue engineering for modelling endometrial diseases and infertility is frustrated by the inability to mimic the fibronectin (FN) extracellular matrix required by human endometrial stromal cells (EnSCs). Here we show tha
Autor:
Christopher F. van der Walle, Michaela Kreiner, Helen J. Mardon, Zhanfeng Cui, Zhaohui Li, RuAngelie Edrada-Ebel
Publikováno v:
Journal of biomedical materials research. Part A. 99(2)
A method to functionalize alginate by introducing monomeric or self-assembling (tetrameric) fibronectin (FN) domains is described, leading to a functional scaffold, which is used for three dimensional (3D) culture of human endometrial stromal cells (