Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Michael-Paul Robinson"'
Autor:
Michael-Paul Robinson, Jinjoo Jung, Natalia Lopez-Barbosa, Matthew Chang, Mingji Li, Thapakorn Jaroentomeechai, Emily C. Cox, Xiaolu Zheng, Mehmet Berkmen, Matthew P. DeLisa
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Abstract Here we describe a facile and robust genetic selection for isolating full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of redox-engineered Escherichia coli cells. The method is based on the transport of a bif
Externí odkaz:
https://doaj.org/article/de5dd8828395498fa9ebf9a6b2a097c0
Autor:
Matthew Wook Chang, Mehmet Berkmen, Xiaolu Zheng, Emily C. Cox, Michael-Paul Robinson, Thapakorn Jaroentomeechai, Matthew P. DeLisa, Mingji Li
We describe a facile and robust genetic selection for isolating full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of the genetically engineered Escherichia coli strain, SHuffle. The method is based on the transport of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73839139a799deb285f0f73188304a70
https://doi.org/10.1101/2020.05.09.085944
https://doi.org/10.1101/2020.05.09.085944
Publikováno v:
Nature chemical biology
Escherichia coli DsbB is a transmembrane enzyme that catalyzes the re-oxidation of the periplasmic oxidase DsbA by ubiquinone. Here, we sought to convert membrane-bound DsbB into a water-soluble biocatalyst by leveraging a previously described method
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25c38e67c3280e993db3b96bdb0032b9
https://doi.org/10.1038/nchembio.2409
https://doi.org/10.1038/nchembio.2409
Autor:
Corinna Tuckey, Christopher J. Noren, Cristen Peterson, Na Ke, Mehmet Berkmen, Thomas J. Mansell, Alana Szkodny, Michael Paul Robinson, Paul A. Colussi, Paul Riggs, Christopher H. Taron, Matthew P. DeLisa, Julie Lobstein
Publikováno v:
Nature Communications
Current methods for producing immunoglobulin G (IgG) antibodies in engineered cells often require refolding steps or secretion across one or more biological membranes. Here, we describe a robust expression platform for biosynthesis of full-length IgG
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13c424707ae17b70f2004fe62d6140ec
https://doi.org/10.1038/ncomms9072
https://doi.org/10.1038/ncomms9072
Publikováno v:
The journal of physical chemistry. B. 114(33)
Applied mechanical force (f) can activate conformational change in molecules by reducing the height of a free-energy barrier (DeltaG(b)). In this paper, molecular dynamics simulations are carried out with umbrella sampling and self-consistent histogr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ed5b9feafcedf87831e2ccc6609416a
https://pubmed.ncbi.nlm.nih.gov/20681524
https://pubmed.ncbi.nlm.nih.gov/20681524
Autor:
Michael-Paul Robinson, Daniel J. Lacks
Publikováno v:
Physical Review E. 77
Molecular dynamics simulations are carried out for slabs of silica liquid with thicknesses between 1 and $3\phantom{\rule{0.3em}{0ex}}\mathrm{nm}$. A local analysis of the Born contribution to the elastic modulus, ${\mathrm{C}}^{\mathrm{B}}$, shows t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9a6ce16d51d44259e9592ffe07e5c0e8
https://doi.org/10.1103/physreve.77.041504
https://doi.org/10.1103/physreve.77.041504