Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Michael Widmann"'
Autor:
Michael Widmann, Jürgen Pleiss
Publikováno v:
PLoS ONE, Vol 9, Iss 7, p e101813 (2014)
Genome and metagenome sequencing projects support the view that only a tiny portion of the total protein microdiversity in the biosphere has been sequenced yet, while the vast majority of existing protein variants is still unknown. By using a network
Externí odkaz:
https://doaj.org/article/18729db981094c35bee376d3da75bfba
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 2, Iss 3 (2012)
In this mini-review we describe the different strategies for rational protein engineering and summarize the computational tools available. Computational tools can either be used to design focused libraries, to predict sequence-function relationships
Externí odkaz:
https://doaj.org/article/fb4a06dfa5c34a17a8dd9792f70b68eb
Publikováno v:
PLoS ONE, Vol 5, Iss 5, p e10546 (2010)
BACKGROUND: Binding of proteins in ion exchange chromatography is dominated by electrostatic interactions and can be tuned by adjusting pH and ionic strength of the solvent. Therefore, the isoelectric region (IER), the pH region of almost zero charge
Externí odkaz:
https://doaj.org/article/015ae0eb9138420486cdb70bc680c8aa
Publikováno v:
Antimicrobial Agents and Chemotherapy. 60:2709-2717
The Lactamase Engineering Database ( www.LacED.uni-stuttgart.de ) was developed to facilitate the classification and analysis of TEM β-lactamases. The current version contains 474 TEM variants. Two hundred fifty-nine variants form a large scale-free
Autor:
Ursula Mackfeld, Doris Hahn, Jürgen Pleiss, Constantin Vogel, Michael Widmann, Martina Pohl, Robert Westphal, Dörte Rother, Michael Müller, Simon Waltzer, Maryam Beigi
Publikováno v:
ChemCatChem. 5:3587-3594
The thiamine diphosphate (ThDP)-dependent enzyme 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase from Escherichia coli (EcMenD, E.C. 2.2.1.9) catalyzes the carboligation of α-ketoglutarate (α-KG) and various benzaldehyde der
Publikováno v:
Antimicrobial Agents and Chemotherapy. 56:3481-3491
Metallo-β-lactamases (MBLs) are enzymes that hydrolyze β-lactam antibiotics, resulting in bacterial resistance to these drugs. These proteins have caused concerns due to their facile transference, broad substrate spectra, and the absence of clinica
Publikováno v:
Semiconductor Science and Technology. 18:434-441
We present simulations of potential landscapes for electrons in random and correlated distributions of repulsive scatterers, which enable the determination of percolation thresholds, densities of states and oscillator strengths. These simulations elu
Publikováno v:
Physica E: Low-dimensional Systems and Nanostructures. 12:662-665
The transition of two-dimensional electron systems (2DES) with intentional disorder in form of a δ-layer doped with beryllium into quantum Hall insulators composed of separate electron droplets is examined by magnetotransport and far-infrared spectr
Publikováno v:
Physica B: Condensed Matter. :762-766
The line shape and position of cyclotron resonance in gated GaAs/GaAlAs heterojunctions with δ -doped layers of negatively charged beryllium acceptors, that provide strong potential fluctuations in the channels of the quasi-two-dimensional electron