Zobrazeno 1 - 10
of 68
pro vyhledávání: '"Michael W. Martynowycz"'
Publikováno v:
IUCrJ, Vol 10, Iss 4, Pp 430-436 (2023)
In this work, a novel crystal growth method termed suspended drop crystallization has been developed. Unlike traditional methods, this technique involves mixing protein and precipitant directly on an electron microscopy grid without any additional su
Externí odkaz:
https://doaj.org/article/68984af222c643ccbbc98efd18555d28
Autor:
Michael W. Martynowycz, Anna Shiriaeva, Max T. B. Clabbers, William J. Nicolas, Sara J. Weaver, Johan Hattne, Tamir Gonen
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Here, authors demonstrate a method for milling vitrified biological material. Using correlative fluorescence and electron microscopy images, MicroED data is collected for the adenosine receptor.
Externí odkaz:
https://doaj.org/article/16fb1d2c79834624ba0f41611c4745a4
Autor:
Alexander M. Wolff, Iris D. Young, Raymond G. Sierra, Aaron S. Brewster, Michael W. Martynowycz, Eriko Nango, Michihiro Sugahara, Takanori Nakane, Kazutaka Ito, Andrew Aquila, Asmit Bhowmick, Justin T. Biel, Sergio Carbajo, Aina E. Cohen, Saul Cortez, Ana Gonzalez, Tomoya Hino, Dohyun Im, Jake D. Koralek, Minoru Kubo, Tomas S. Lazarou, Takashi Nomura, Shigeki Owada, Avi J. Samelson, Tomoyuki Tanaka, Rie Tanaka, Erin M. Thompson, Henry van den Bedem, Rahel A. Woldeyes, Fumiaki Yumoto, Wei Zhao, Kensuke Tono, Sebastien Boutet, So Iwata, Tamir Gonen, Nicholas K. Sauter, James S. Fraser, Michael C. Thompson
Publikováno v:
IUCrJ, Vol 7, Iss 2, Pp 306-323 (2020)
Innovative new crystallographic methods are facilitating structural studies from ever smaller crystals of biological macromolecules. In particular, serial X-ray crystallography and microcrystal electron diffraction (MicroED) have emerged as useful me
Externí odkaz:
https://doaj.org/article/6e6b471c98104285badfe50aab472279
Publikováno v:
Journal of Structural Biology: X, Vol 6, Iss , Pp 100078- (2022)
Microcrystal electron diffraction (MicroED) is a powerful technique utilizing electron cryo-microscopy (cryo-EM) for protein structure determination of crystalline samples too small for X-ray crystallography. Electrons interact with the electrostatic
Externí odkaz:
https://doaj.org/article/5a4c0d4f435845dcb8494c6aba87f2d8
Autor:
Michael W. Martynowycz, Tamir Gonen
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100686- (2021)
Summary: We present an in-depth protocol to reproducibly prepare crystalline lamellae from protein crystals for subsequent microcrystal electron diffraction (MicroED) experiments. This protocol covers typical soluble proteins and membrane proteins em
Externí odkaz:
https://doaj.org/article/7805d4db07264800aa57a7e69e56978c
Publikováno v:
IUCrJ, Vol 6, Iss 5, Pp 921-926 (2019)
Microcrystal electron diffraction (MicroED) combines crystallography and electron cryo-microscopy (cryo-EM) into a method that is applicable to high-resolution structure determination. In MicroED, nanosized crystals, which are often intractable using
Externí odkaz:
https://doaj.org/article/63f09f2e6aeb443eb21cc6704feb0491
Autor:
Christopher G. Jones, Michael W. Martynowycz, Johan Hattne, Tyler J. Fulton, Brian M. Stoltz, Jose A. Rodriguez, Hosea M. Nelson, Tamir Gonen
Publikováno v:
ACS Central Science, Vol 4, Iss 11, Pp 1587-1592 (2018)
Externí odkaz:
https://doaj.org/article/804f116afbda4e23b71f6e32e189a3b1
Autor:
Fazle R. Dayeen, Bret A. Brandner, Michael W. Martynowycz, Kamil Kucuk, Michael J. Foody, Wei Bu, Stephen B. Hall, David Gidalevitz
Publikováno v:
Biophysical Journal. 121:3533-3541
Cholesterol induces faster collapse by compressed films of pulmonary surfactant. Because collapse prevents films from reaching the high surface pressures achieved in the alveolus, most therapeutic surfactants remove or omit cholesterol. The studies h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::162c09c1eac6802ebdfc809d939cbb4a
https://doi.org/10.1016/j.bpj.2022.07.007
https://doi.org/10.1016/j.bpj.2022.07.007
Autor:
Michael W. Martynowycz, Tamir Gonen
Publikováno v:
Physics Today. 75:38-42
Structural biologists are using cryogenic electron microscopy to resolve atomic-scale structures of proteins from nanocrystals.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aa48d5604b47ff9c0142509c47bdc62d
https://doi.org/10.1063/pt.3.5019
https://doi.org/10.1063/pt.3.5019
Publikováno v:
bioRxiv
SUMMARYMicrocrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::218e3043d9fddb8004db6e7150e1f70c
https://europepmc.org/articles/PMC10168392/
https://europepmc.org/articles/PMC10168392/
