Top-Down Atmospheric Ionization Mass Spectrometry Microscopy Combined With Proteogenomics

Autor: Terry Gaasterland, Michael J. Meehan, Pieter C. Dorrestein, Cheng-Chih Hsu, Michael W. Baker, Eduardo R. Macagno

Publikováno v: Analytical chemistry, vol 89, iss 16
Hsu, C-C; Baker, MW; Gaasterland, T; Meehan, MJ; Macagno, ER; & Dorrestein, PC. (2017). Top-Down Atmospheric Ionization Mass Spectrometry Microscopy Combined With Proteogenomics. ANALYTICAL CHEMISTRY, 89(16), 8251-8258. doi: 10.1021/acs.analchem.7b01096. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/32072890

Mass spectrometry-based protein analysis has become an important methodology for proteogenomic mapping by providing evidence for the existence of proteins predicted at the genomic level. However, screening and identification of proteins directly on t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5bd494a2a97ac782920b1b4456293fe7
https://doi.org/10.1021/acs.analchem.7b01096

Purinergic modulation of neuronal gap junction circuits in the CNS of the leech

Autor: Martha J. Solano, Vinh‐Vincent Hua, Lina Abdulnoor, Michael W. Baker, Eduardo R. Macagno, Oliva Mota Segura

Publikováno v: Journal of neuroscience researchREFERENCES. 98(6)

Gap junctions (GJs) are widely distributed in brains across the animal kingdom. To visualize the GJ- coupled networks of two major mechanosensory neurons in the ganglia of medicinal leeches, we injected these cells with the GJ-permeable tracer Neurob

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73d135c3ccf71522df7cebc682142f40
https://pubmed.ncbi.nlm.nih.gov/32096570

Expression of a dominant negative mutant innexin in identified neurons and glial cells reveals selective interactions among gap junctional proteins

Autor: Constantine P. Firme, Eduardo R. Macagno, Neema Yazdani, Michael W. Baker

Publikováno v: Developmental Neurobiology. 73:571-586

Neurons and glia of the medicinal leech CNS express different subsets of the 21 innexin genes encoded in its genome. We report here that the punctal distributions of fluorescently tagged innexin transgenes varies in a stereotypical pattern depending

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ff5120df274289e017f7882f275eefb0
https://doi.org/10.1002/dneu.22082

The medicinal leech genome encodes 21 innexin genes: different combinations are expressed by identified central neurons

Autor: Terry Gaasterland, Eunice Kym, Michael W. Baker, Allan Wu, Neema Yazdani, Brandon Kandarian, Alejandro Sanchez, Jasmine Sethi, Eduardo R. Macagno, Lee Edsall

Publikováno v: Development Genes and Evolution. 222:29-44

Gap junctional proteins are important components of signaling pathways required for the development and ongoing functions of all animal tissues, particularly the nervous system, where they function in the intracellular and extracellular exchange of s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44b2603df0d5277e6f4fb073726bdeea
https://doi.org/10.1007/s00427-011-0387-z

The receptor phosphatase HmLAR2 collaborates with focal adhesion proteins in filopodial tips to control growth cone morphology

Autor: Eduardo R. Macagno, Michael W. Baker, Sandra M. Peterson

Publikováno v: Developmental Biology. 320(1):215-225

Receptor protein tyrosine phosphatases (RPTPs) have been shown to play key roles in regulating axon guidance and synaptogenesis. HmLAR2, one of two closely related LAR-like RPTPs in the embryonic leech, is expressed in a few central neurons and in a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df19aafd0aa4329e0c93b1a6e1a5a486