Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA

Autor: Michael Stangegaard, Inge Høgh Dufva, Martin Dufva

Publikováno v: BioTechniques, Vol 40, Iss 5, Pp 649-657 (2006)

Reverse transcription of RNA is an invaluable method for gene expression analysis by real-time PCR or microarray methods. Random primers of varying lengths were compared with respect to their efficiency of priming reverse transcription reactions. The

Externí odkaz: https://doaj.org/article/7fcae8de65fb4dd7aa43ba7e00672afd

Automated Extraction of DNA from Blood and PCR Setup using a Tecan Freedom EVO Liquid Handler for Forensic Genetic STR Typing of Reference Samples

Autor: Anders J. Hansen, Michael Stangegaard, Niels Morling, Rune Frank-Hansen, Tobias Guldberg Frøslev

Publikováno v: JALA: Journal of the Association for Laboratory Automation. 16:134-140

We have implemented and validated automated protocols for DNA extraction and PCR setup using a Tecan Freedom EVO liquid handler mounted with the Te-MagS magnetic separation device (Tecan, Mannedorf, Switzerland). The protocols were validated for accr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8d2ba860905de13f572e238f81316df
https://doi.org/10.1016/j.jala.2010.11.003

Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA

Autor: Martin Dufva, Inge Høgh Dufva, Michael Stangegaard

Publikováno v: BioTechniques. 40:649-657

Reverse transcription of RNA is an invaluable method for gene expression analysis by real-time PCR or microarray methods. Random primers of varying lengths were compared with respect to their efficiency of priming reverse transcription reactions. The

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1279aa4326c7517e3adf0da00ec6c378
https://doi.org/10.2144/000112153

Customizing a commercial laboratory information management system for a forensic genetic laboratory

Autor: Lynge C. Christiansen, B.T. Simonsen, Charlotte Hallenberg, Sigrun Dalsgaard, Addie Nina Olsen, Anders J. Hansen, Niels Morling, Steffen J. Nielsen, Michael Stangegaard, Rune Frank-Hansen, Ulrikke J.M. Willerslev, Torben Madsen, Claus Børsting

Publikováno v: Forensic Science International: Genetics Supplement Series. 2:77-79

The need for high-throughput laboratories to comply with regulatory requirements makes data management an important aspect of forensic genetics. A Laboratory Information Management System (LIMS) enables efficient workflows and ensures traceability if

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::44b83fc5c509863ecdf7a7baf4a25c01
https://doi.org/10.1016/j.fsigss.2009.07.012

Evaluation of Four Automated Protocols for Extraction of DNA from FTA Cards

Autor: Claus Børsting, Michael Stangegaard, Rune Frank-Hansen, Laura Ferrero-Miliani, Niels Morling, Anders J. Hansen, Lena Poulsen

Publikováno v: Stangegaard, M, Børsting, C, Ferrero-Miliani, L, Frank-Hansen, R, Poulsen, L, Hansen, A J & Morling, N 2013, ' Evaluation of Four Automated Protocols for Extraction of DNA from FTA Cards ', SLAS TECHNOLOGY: Translating Life Sciences Innovation, vol. 18, pp. 404-410 . https://doi.org/10.1177/2211068213484472

Extraction of DNA using magnetic bead-based techniques on automated DNA extraction instruments provides a fast, reliable, and reproducible method for DNA extraction from various matrices. Here, we have compared the yield and quality of DNA extracted

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ac83fe226eedec0f806297ac03d4aea
https://curis.ku.dk/portal/da/publications/evaluation-of-four-automated-protocols-for-extraction-of-dna-from-fta-cards(1f5c74da-de4c-4c30-b3ca-5535b58ca1db).html