Zobrazeno 1 - 10
of 55
pro vyhledávání: '"Michael Shales"'
Autor:
Fernando Meza Gutierrez, Deniz Simsek, Arda Mizrak, Adam Deutschbauer, Hannes Braberg, Jeffrey Johnson, Jiewei Xu, Michael Shales, Michelle Nguyen, Raquel Tamse-Kuehn, Curt Palm, Lars M Steinmetz, Nevan J Krogan, David P Toczyski
Publikováno v:
eLife, Vol 7 (2018)
Although polyubiquitin chains linked through all lysines of ubiquitin exist, specific functions are well-established only for lysine-48 and lysine-63 linkages in Saccharomyces cerevisiae. To uncover pathways regulated by distinct linkages, genetic in
Externí odkaz:
https://doaj.org/article/f07665d4c7984c8e8770d5baaa41a25f
Autor:
Humberto Martin, Michael Shales, Pablo Fernandez‐Piñar, Ping Wei, Maria Molina, Dorothea Fiedler, Kevan M Shokat, Pedro Beltrao, Wendell Lim, Nevan J Krogan
Publikováno v:
Molecular Systems Biology, Vol 11, Iss 4, Pp 1-13 (2015)
Abstract Genetic interaction screens have been applied with great success in several organisms to study gene function and the genetic architecture of the cell. However, most studies have been performed under optimal growth conditions even though many
Externí odkaz:
https://doaj.org/article/377f4684244e4ad194f4cd67d054bb98
Autor:
Joseph I. Kliegman, Dorothea Fiedler, Colm J. Ryan, Yi-Fan Xu, Xiao-yang Su, David Thomas, Max C. Caccese, Ada Cheng, Michael Shales, Joshua D. Rabinowitz, Nevan J. Krogan, Kevan M. Shokat
Publikováno v:
Cell Reports, Vol 5, Iss 6, Pp 1725-1736 (2013)
Current approaches for identifying synergistic targets use cell culture models to see if the combined effect of clinically available drugs is better than predicted by their individual efficacy. New techniques are needed to systematically and rational
Externí odkaz:
https://doaj.org/article/6e8a50a061a746a3bc5414fd47db57d1
Autor:
James E. Haber, Hannes Braberg, Qiuqin Wu, Richard Alexander, Julian Haase, Colm Ryan, Zach Lipkin-Moore, Kathleen E. Franks-Skiba, Tasha Johnson, Michael Shales, Tineke L. Lenstra, Frank C.P. Holstege, Jeffrey R. Johnson, Kerry Bloom, Nevan J. Krogan
Publikováno v:
Cell Reports, Vol 3, Iss 6, Pp 2168-2178 (2013)
Genetic interactions reveal the functional relationships between pairs of genes. In this study, we describe a method for the systematic generation and quantitation of triple mutants, termed triple-mutant analysis (TMA). We have used this approach to
Externí odkaz:
https://doaj.org/article/0ee5fd978f00418b9e5d751d398e6417
Autor:
Jiashun Zheng, Joris J Benschop, Michael Shales, Patrick Kemmeren, Jack Greenblatt, Gerard Cagney, Frank Holstege, Hao Li, Nevan J Krogan
Publikováno v:
Molecular Systems Biology, Vol 6, Iss 1, Pp 1-12 (2010)
Abstract The regulation of gene expression is, in large part, mediated by interplay between the general transcription factors (GTFs) that function to bring about the expression of many genes and site‐specific DNA‐binding transcription factors (ST
Externí odkaz:
https://doaj.org/article/b8e47565a7844e6393c7a94ef43ea817
Autor:
Kristin L Patrick, Colm J Ryan, Jiewei Xu, Jesse J Lipp, Kelly E Nissen, Assen Roguev, Michael Shales, Nevan J Krogan, Christine Guthrie
Publikováno v:
PLoS Genetics, Vol 11, Iss 3, p e1005074 (2015)
Although numerous regulatory connections between pre-mRNA splicing and chromatin have been demonstrated, the precise mechanisms by which chromatin factors influence spliceosome assembly and/or catalysis remain unclear. To probe the genetic network of
Externí odkaz:
https://doaj.org/article/9641aa827e364c8e99254de0f3d90149
Autor:
Jeffrey R. Johnson, David C. Crosby, Judd F. Hultquist, Andrew P. Kurland, Prithy Adhikary, Donna Li, John Marlett, Justine Swann, Ruth Hüttenhain, Erik Verschueren, Tasha L. Johnson, Billy W. Newton, Michael Shales, Viviana A. Simon, Pedro Beltrao, Alan D. Frankel, Alexander Marson, Jeffery S. Cox, Oliver I. Fregoso, John A.T. Young, Nevan J. Krogan
Publikováno v:
Cell reports, vol 39, iss 2
Cell Reports, 39 (2)
Cell Reports, 39 (2)
Viruses must effectively remodel host cellular pathways to replicate and evade immune defenses, and they must do so with limited genomic coding capacity. Targeting post-translational modification (PTM) pathways provides a mechanism by which viruses c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f6761189536151f78dc8ad100c5fe511
Autor:
Richard Alexander, Brian D. Strahl, Shuangying Jiang, John H. Morris, Kaitlin K. Chaung, Raghuvar Dronamraju, Shuyi Wang, James S. Fraser, Derek Bogdanoff, Stefan Bohn, James E. Haber, Peter Cimermancic, Michael Shales, Nevan J. Krogan, Anthony L. Shiver, Riccardo Pellarin, Jef D. Boeke, Hannes Braberg, David Mavor, Junbiao Dai, Jiewei Xu, Andrej Sali, Ignacia Echeverria, Joel S. Bader, Ruth Hüttenhain, Gajendradhar Dwivedi, Carol A. Gross, Dina Schneidman
Publikováno v:
Science (New York, N.Y.), vol 370, iss 6522
Science
Science
From phenotype to structure Much insight has come from structures of macromolecular complexes determined by methods such as crystallography or cryo–electron microscopy. However, looking at transient complexes remains challenging, as does determinin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99d0f10bdfb513e43da5009d574b06bf
https://escholarship.org/uc/item/43n670zm
https://escholarship.org/uc/item/43n670zm
Autor:
David C. Crosby, Assen Roguev, Joshua Kane, Michael Shales, Jiewei Xu, Erica Stevenson, Ariane Watson, Alexander Marson, David E. Gordon, Ivan Marazzi, Nevan J. Krogan, Gwendolyn M. Jang, Simin Zheng, Jeffrey Z. Guo, Danielle L. Swaney, Alan D. Frankel, Erik Verschueren, Gerard Cagney, Kathy Franks-Skiba
Publikováno v:
Molecular cell, vol 78, iss 2
Mol Cell
Mol Cell
We have developed a platform for quantitative genetic interaction mapping using viral infectivity as a functional readout and constructed a viral host-dependency epistasis map (vE-MAP) of 356 human genes linked to HIV function, comprising >63,000 pai
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8289265b3b0e39fd524b7ea07648e0d0
https://escholarship.org/uc/item/75v3v7mq
https://escholarship.org/uc/item/75v3v7mq
Autor:
Lei S. Qi, Michael Shales, Trey Ideker, Assen Roguev, John Paul Shen, Meng Chen, Prashant Mali, Nevan J. Krogan, Si-Han Chen, Dan Du, David E. Gordon
Publikováno v:
Nature Methods. 14:577-580
CRISPR interference screens with pairs of sgRNAs allow for quantitative characterization of genetic interactions. We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We tar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::654a4f561aafc88bac4dd6fd759b6b5a
https://doi.org/10.1038/nmeth.4286
https://doi.org/10.1038/nmeth.4286