Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Michael S. Woody"'
Autor:
Michael S. Woody, Michael J. Greenberg, Bipasha Barua, Donald A. Winkelmann, Yale E. Goldman, E. Michael Ostap
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
Omecamtiv mecarbil (OM) is a positive cardiac inotrope in clinical trials for the treatment of heart failure whose mechanism of action is incompletely understood. Here the authors show that OM inhibits myosin's working stroke and prolongs actomyosin
Externí odkaz:
https://doaj.org/article/a390d9a0840145559f69f13c5f0635e6
Publikováno v:
eLife, Vol 8 (2019)
Key steps of cardiac mechanochemistry, including the force-generating working stroke and the release of phosphate (Pi), occur rapidly after myosin-actin attachment. An ultra-high-speed optical trap enabled direct observation of the timing and amplitu
Externí odkaz:
https://doaj.org/article/7e6497584f8c4b5f9f10fe21aaa0176a
Autor:
Lucia Gardini, Michael S. Woody, Anatolii V. Kashchuk, Yale E. Goldman, E. Michael Ostap, Marco Capitanio
Publikováno v:
Optical Tweezers ISBN: 9781071622285
Methods Mol Biol
Methods Mol Biol
Interactions between biological molecules occur on very different time scales, from the minutes of strong protein-protein bonds, down to below the millisecond duration of rapid biomolecular interactions. Conformational changes occurring on sub-ms tim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::635db94da742fa9e4d6a71889a1034bc
https://doi.org/10.1007/978-1-0716-2229-2_19
https://doi.org/10.1007/978-1-0716-2229-2_19
Publikováno v:
Biophysical Journal. 112:1214-1220
Temporal sequences of fluorescence intensities in single-molecule experiments are often obtained from stacks of camera images. The dwell times of different macromolecular structural or functional states, correlated with characteristic fluorescence in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39b29bcfa828e80a3c61f0b11f3d26bb
https://doi.org/10.1016/j.bpj.2017.02.014
https://doi.org/10.1016/j.bpj.2017.02.014
Autor:
Brandon M. Bensel, Yale E. Goldman, Michael S. Woody, E. Michael Ostap, Serapion Pyrpassopoulos, Susan P. Gilbert
Publikováno v:
Biophysical Journal. 118:429a-430a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::167755a2d57b0217a395e19e7ceb6538
https://doi.org/10.1016/j.bpj.2019.11.2415
https://doi.org/10.1016/j.bpj.2019.11.2415
Publikováno v:
eLife, Vol 8 (2019)
eLife
eLife
Key steps of cardiac mechanochemistry, including the force-generating working stroke and the release of phosphate (Pi), occur rapidly after myosin-actin attachment. An ultra-high-speed optical trap enabled direct observation of the timing and amplitu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::109459997520687eab365de1116d8d5d
https://doi.org/10.7554/elife.49266
https://doi.org/10.7554/elife.49266
Publikováno v:
Biophysical Journal. 111:273-282
We present MEMLET (MATLAB-enabled maximum-likelihood estimation tool), a simple-to-use and powerful program for utilizing maximum-likelihood estimation (MLE) for parameter estimation from data produced by single-molecule and other biophysical experim
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0582154929477e67c8548bc1ce5448c7
https://doi.org/10.1016/j.bpj.2016.06.019
https://doi.org/10.1016/j.bpj.2016.06.019
Autor:
Bipasha Barua, E. Michael Ostap, Michael J. Greenberg, Yale E. Goldman, Donald A. Winkelmann, Michael S. Woody
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
Omecamtiv mecarbil (OM) is a positive cardiac inotrope in phase-3 clinical trials for treatment of heart failure. Although initially described as a direct myosin activator, subsequent studies are at odds with this description and do not explain OM-me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b7e7bce9957c26244a267d77ca36a53
Publikováno v:
Optical Trapping and Optical Micromanipulation XIV.
We assembled an ultra-fast infrared optical trapping system to detect mechanical events that occur less than a millisecond after a ligand binds to its filamentous substrate, such as myosin undergoing its 5 – 10 nm working stroke after actin binding
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c29aad093f1fbd038ac75e5cd2a46436
https://doi.org/10.1117/12.2277130
https://doi.org/10.1117/12.2277130