Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Michael S. Akhras"'
Autor:
Michael S Akhras, Erik Pettersson, Lisa Diamond, Magnus Unemo, Jennifer Okamoto, Ronald W Davis, Nader Pourmand
Publikováno v:
PLoS ONE, Vol 8, Iss 10, p e76696 (2013)
Here we describe the novel Sequencing Bead Array (SBA), a complete assay for molecular diagnostics and typing applications. SBA is a digital suspension array using Next-Generation Sequencing (NGS), to replace conventional optical readout platforms. T
Externí odkaz:
https://doaj.org/article/ed06c28cdf8f4ebb8bd587feadeabeca
Autor:
Michael S Akhras, Magnus Unemo, Sreedevi Thiyagarajan, Pål Nyrén, Ronald W Davis, Andrew Z Fire, Nader Pourmand
Publikováno v:
PLoS ONE, Vol 2, Iss 9, p e915 (2007)
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which e
Externí odkaz:
https://doaj.org/article/5e957c84cac440b58e8f5225d3a312cb
Autor:
Michael S Akhras, Sreedevi Thiyagarajan, Andrea C Villablanca, Ronald W Davis, Pål Nyrén, Nader Pourmand
Publikováno v:
PLoS ONE, Vol 2, Iss 2, p e223 (2007)
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection. We have demonst
Externí odkaz:
https://doaj.org/article/883728b1dfbb4f60b6c07f784260507f
Autor:
Michael S. Akhras, Nader Pourmand, Emma Lindbäck, Anneli Sjöstrand, Baback Gharizadeh, Bengt Wretlind, Magnus Unemo, Hans Fredlund
Publikováno v:
APMIS. 115:231-241
A highly discriminative and objective genetic characterization of N. gonorrhoeae, which increases our knowledge of strain populations in different geographic areas, is crucial for the development of improved control measures. In the present study, co
Autor:
Nader Pourmand, Björn Strander, Baback Gharizadeh, Biying Zheng, Pål Nyrén, Michael S. Akhras, Keng-Ling Wallin, Mehran Ghaderi, Olufisayo Jejelowo
Publikováno v:
Molecular and Cellular Probes. 20:230-238
Despite the various technologies in place for genotyping human papillomaviruses (HPV), clinical use and clinical research demand a method that is fast, more reliable and cost-effective. The technology described here represents a breakthrough developm
Autor:
Baback Gharizadeh, Pål Nyrén, Nader Pourmand, Magnus Unemo, Michael S. Akhras, Bengt Wretlind
Publikováno v:
International Journal of Antimicrobial Agents. 26:486-490
Quinolone resistance is rapidly increasing in Neisseria gonorrhoeae and is posing a significant public health threat that requires constant surveillance. A rapid and reliable mutation detection assay has been developed. The assay is based on pre-prog
Publikováno v:
Journal of biotechnology, vol 167, iss 4
Super-paramagnetic beads (SPMB)s used for a variety of molecular diagnostic assays are prepared by attaching pre-synthesized oligonucleotides to the surface via a cumbersome and low efficient method of carbodiimide-mediated amide bond formation. To m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::873ce205bf9a83f4d7bda2369ec4a8cb
https://escholarship.org/uc/item/2x03z495
https://escholarship.org/uc/item/2x03z495
Autor:
Heng Yu, Sebastian J. Osterfeld, Michael S. Akhras, Shu-Jen Han, Robert L. White, Nader Pourmand, Liang Xu, Shan X. Wang
Publikováno v:
Biosensorsbioelectronics. 24(1)
A giant magnetoresistive (GMR) biochip based on spin valve sensor array and magnetic nanoparticle labels was developed for inexpensive, sensitive and reliable DNA detection. The DNA targets detected in this experiment were PCR products amplified from
Autor:
Pål Nyrén, Ronald W. Davis, Michael S. Akhras, Sreedevi Thiyagarajan, Andrew Fire, Nader Pourmand, Magnus Unemo
Publikováno v:
PLoS ONE, Vol 2, Iss 9, p e915 (2007)
PLoS ONE
PLoS ONE
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system. As a proof-of-concept, this novel method, which e
Autor:
Magnus Unemo, Bengt Wretlind, Michael S. Akhras, Nader Pourmand, Baback Gharizadeh, Hans Fredlund, Emma Lindbäck
Publikováno v:
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 114(12)
The quinolone resistance determining region (QRDR) of the gyrA gene in ciprofloxacin-susceptible strains (n=53) and strains of Neisseria spp. with reduced susceptibility (n=70) was determined by the pyrosequencing method. Results showed that the QRDR