Zobrazeno 1 - 10
of 64
pro vyhledávání: '"Michael S Humphrys"'
Autor:
Michael S Humphrys, Todd Creasy, Yezhou Sun, Amol C Shetty, Marcus C Chibucos, Elliott F Drabek, Claire M Fraser, Umar Farooq, Naomi Sengamalay, Sandy Ott, Huizhong Shou, Patrik M Bavoil, Anup Mahurkar, Garry S A Myers
Publikováno v:
PLoS ONE, Vol 8, Iss 12, p e80597 (2013)
We developed an RNA-Seq-based method to simultaneously capture prokaryotic and eukaryotic expression profiles of cells infected with intracellular bacteria. As proof of principle, this method was applied to Chlamydia trachomatis-infected epithelial c
Externí odkaz:
https://doaj.org/article/59def294e3484609874a64f23c588b1b
Autor:
Michael T. France, Li Fu, Lindsay Rutt, Hongqiu Yang, Michael S. Humphrys, Shilpa Narina, Pawel M. Gajer, Bing Ma, Larry J. Forney, Jacques Ravel
Publikováno v:
Genome Biology, Vol 23, Iss 1, Pp 1-26 (2022)
Abstract Background Vaginal bacterial communities dominated by Lactobacillus species are associated with a reduced risk of various adverse health outcomes. However, somewhat unexpectedly, many healthy women have microbiota that are not dominated by l
Externí odkaz:
https://doaj.org/article/e510f20c46f24b1f88ead36c48a9198b
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
Abstract Dual RNA-seq experiments examining viral and bacterial pathogens are increasing, but vary considerably in their experimental designs, such as infection rates and RNA depletion methods. Here, we have applied dual RNA-seq to Chlamydia trachoma
Externí odkaz:
https://doaj.org/article/fe8a5218d62e4a1f995962750e59e982
Autor:
Regan J. Hayward, James W. Marsh, Michael S. Humphrys, Wilhelmina M. Huston, Garry S. A. Myers
Publikováno v:
Epigenetics & Chromatin, Vol 13, Iss 1, Pp 1-18 (2020)
Abstract Chlamydia are Gram-negative, obligate intracellular bacterial pathogens responsible for a broad spectrum of human and animal diseases. In humans, Chlamydia trachomatis is the most prevalent bacterial sexually transmitted infection worldwide
Externí odkaz:
https://doaj.org/article/8dd81036c68840eb8c3d1b87a86c6fe2
Autor:
Bing Ma, Michael T. France, Jonathan Crabtree, Johanna B. Holm, Michael S. Humphrys, Rebecca M. Brotman, Jacques Ravel
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Reference databases are essential for studies on host-microbiota interactions. Here, the authors present the construction of VIRGO, a human vaginal non-redundant gene catalog, which represents a comprehensive resource for taxonomic and functional pro
Externí odkaz:
https://doaj.org/article/e43046f756014843ab0d63a2124e81f7
Autor:
Michal A. Elovitz, Pawel Gajer, Valerie Riis, Amy G. Brown, Michael S. Humphrys, Johanna B. Holm, Jacques Ravel
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
Here, Elovitz et al. investigate associations between cervicovaginal microbiota (CVM) and spontaneous preterm birth (sPTB) in a large cohort of African American and non-African American women, and find that CVM and local immune response early in preg
Externí odkaz:
https://doaj.org/article/f079386160224b2f96baee4839409544
Autor:
Regan J. Hayward, James W. Marsh, Michael S. Humphrys, Wilhelmina M. Huston, Garry S. A. Myers
Publikováno v:
Frontiers in Cellular and Infection Microbiology, Vol 9 (2019)
Chlamydia are Gram-negative obligate intracellular bacterial pathogens responsible for a variety of disease in humans and animals worldwide. Chlamydia trachomatis causes trachoma in disadvantaged populations, and is the most common bacterial sexually
Externí odkaz:
https://doaj.org/article/aa905b8da5f240acabdb4a9817f97df5
Autor:
Vonetta L. Edwards, Steven B. Smith, Elias J. McComb, Jeanne Tamarelle, Bing Ma, Michael S. Humphrys, Pawel Gajer, Kathleen Gwilliam, Alison M. Schaefer, Samuel K. Lai, Mishka Terplan, Katrina S. Mark, Rebecca M. Brotman, Larry J. Forney, Patrik M. Bavoil, Jacques Ravel
Publikováno v:
mBio, Vol 10, Iss 4 (2019)
ABSTRACT The mechanism(s) by which Lactobacillus-dominated cervicovaginal microbiota provide a barrier to Chlamydia trachomatis infection remain(s) unknown. Here we evaluate the impact of different Lactobacillus spp. identified via culture-independen
Externí odkaz:
https://doaj.org/article/c7ac43892b7246beabc1cea4bec10970
Autor:
Johanna B. Holm, Michael S. Humphrys, Courtney K. Robinson, Matthew L. Settles, Sandra Ott, Li Fu, Hongqiu Yang, Pawel Gajer, Xin He, Elias McComb, Patti E. Gravitt, Khalil G. Ghanem, Rebecca M. Brotman, Jacques Ravel
Publikováno v:
mSystems, Vol 4, Iss 1 (2019)
ABSTRACT Amplification, sequencing, and analysis of the 16S rRNA gene affords characterization of microbial community composition. As this tool has become more popular and amplicon-sequencing applications have grown in the total number of samples, gr
Externí odkaz:
https://doaj.org/article/5497aa6451b84735bb78bb98f1b0fc8a
Publikováno v:
Frontiers in Microbiology, Vol 8 (2017)
Dual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells. By simultaneously investigating both organisms from the
Externí odkaz:
https://doaj.org/article/cda11281ed4840f9bebff89e433c0e9f