Zobrazeno 1 - 10
of 124
pro vyhledávání: '"Michael S, Krangel"'
Publikováno v:
Frontiers in Immunology, Vol 14 (2023)
V(D)J recombination of antigen receptor loci is a highly developmentally regulated process. During T lymphocyte development, recombination of the Tcra gene occurs in CD4+CD8+ double positive (DP) thymocytes and requires the Tcra enhancer (Eα). E pro
Externí odkaz:
https://doaj.org/article/9a73c81cac134069b26e6412372636f6
Autor:
Mohammad M. Karimi, Ya Guo, Xiaokai Cui, Husayn A. Pallikonda, Veronika Horková, Yi-Fang Wang, Sara Ruiz Gil, Gustavo Rodriguez-Esteban, Irene Robles-Rebollo, Ludovica Bruno, Radina Georgieva, Bhavik Patel, James Elliott, Marian H. Dore, Danielle Dauphars, Michael S. Krangel, Boris Lenhard, Holger Heyn, Amanda G. Fisher, Ondřej Štěpánek, Matthias Merkenschlager
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Developing T cells commit to either CD4/helper or CD8/cytotoxic lineage in the thymus, but how CD4 and CD8 coreceptors and TCR signaling dictate this selection process is still unclear. Here the authors use single cell RNA sequencing of mouse thymocy
Externí odkaz:
https://doaj.org/article/ce6d54e4a05749d99545339b720e286a
Publikováno v:
Cell Reports, Vol 19, Iss 10, Pp 2157-2173 (2017)
Adaptive immunity depends on diverse T cell receptor repertoires generated by variable, diversity, and joining (V[D]J) recombination. Here, we define the principles by which combinatorial diversity is generated in the murine Tcra repertoire. Tcra and
Externí odkaz:
https://doaj.org/article/dca74c6d9a1b4c7fbb9cb36ff7d50660
Autor:
Tina M. Thornton, Pilar Delgado, Liang Chen, Beatriz Salas, Dimitry Krementsov, Miriam Fernandez, Santiago Vernia, Roger J. Davis, Ruth Heimann, Cory Teuscher, Michael S. Krangel, Almudena R. Ramiro, Mercedes Rincón
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
Double stranded DNA breaks are generated during rearrangements of lymphocyte antigen receptors. Here the authors show that the DNA breaks induce phosphorylation of nuclear GSK3β at Ser389/Thr390, protecting the activated lymphocytes from necroptosis
Externí odkaz:
https://doaj.org/article/c6724a6232d643268dc7f38ec902b517
Autor:
Shiwei Chen, Teresa Romeo Luperchio, Xianrong Wong, Europe B. Doan, Aaron T. Byrd, Kingshuk Roy Choudhury, Karen L. Reddy, Michael S. Krangel
Publikováno v:
Cell Reports, Vol 25, Iss 7, Pp 1729-1740.e6 (2018)
Summary: Tcrb locus V(D)J recombination is regulated by positioning at the nuclear periphery. Here, we used DamID to profile Tcrb locus interactions with the nuclear lamina at high resolution. We identified a lamina-associated domain (LAD) border com
Externí odkaz:
https://doaj.org/article/630ebbe68829489bb0b52e0dd6731e9c
Autor:
Salvatore Loguercio, E. Mauricio Barajas-Mora, Han-Yu Shih, Michael S. Krangel, Ann J. Feeney
Publikováno v:
Frontiers in Immunology, Vol 9 (2018)
CCCTC-binding factor (CTCF) is largely responsible for the 3D architecture of the genome, in concert with the action of cohesin, through the creation of long-range chromatin loops. Cohesin is hypothesized to be the main driver of these long-range chr
Externí odkaz:
https://doaj.org/article/1c04b67f6ffa4dc69583191914d4ed6e
Publikováno v:
T-Cell Development ISBN: 9781071627396
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5c28913c58eea52a0e95976b1ee129d5
https://doi.org/10.1007/978-1-0716-2740-2_16
https://doi.org/10.1007/978-1-0716-2740-2_16
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2580
Quantitative real-time PCR and next-generation sequencing (NGS) are invaluable techniques to analyze T cell receptor (Tcr) gene rearrangements in mouse lymphocyte populations. Although these approaches are powerful, they also have limitations that mu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9708ee32c72fcafbaccdf6329dbed425
https://pubmed.ncbi.nlm.nih.gov/36374463
https://pubmed.ncbi.nlm.nih.gov/36374463
Publikováno v:
Journal of Experimental Medicine. 219
The Tcra repertoire is generated by multiple rounds of Vα-Jα rearrangement. However, Tcrd recombination precedes Tcra recombination within the complex Tcra-Tcrd locus. Here, by ablating Tcrd recombination, we report that Tcrd rearrangement broadens
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0a9023f2ce0c1ae00706fa5112e53ce
https://doi.org/10.1084/jem.20211581
https://doi.org/10.1084/jem.20211581
Hierarchical assembly and disassembly of a transcriptionally active RAG locus in CD4+CD8+ thymocytes
Publikováno v:
The Journal of Experimental Medicine
Naik et al. show that GATA3, Runx1, and E2A are essential for hierarchical assembly of a transcriptionally active RAG locus chromatin hub in CD4+CD8+ thymocytes. Signal-dependent down-regulation of RAG expression is associated with hub disassembly an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2440b8af90986c82611586b6d6764ad
https://doi.org/10.1084/jem.20181402
https://doi.org/10.1084/jem.20181402