Zobrazeno 1 - 10
of 366
pro vyhledávání: '"Michael Rosbash"'
Autor:
Nachen Yang, Satyam P. Srivastav, Reazur Rahman, Qicheng Ma, Gargi Dayama, Sizheng Li, Madoka Chinen, Elissa P. Lei, Michael Rosbash, Nelson C. Lau
Publikováno v:
PLoS Genetics, Vol 18, Iss 3 (2022)
Genetic mechanisms that repress transposable elements (TEs) in young animals decline during aging, as reflected by increased TE expression in aged animals. Does increased TE expression during aging lead to more genomic TE copies in older animals? To
Externí odkaz:
https://doaj.org/article/f4b285347b0e44c1b2ba99134bd2bbcb
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100634- (2021)
Summary: Targets of RNA-binding proteins discovered by editing (TRIBE) determines RNA-proteins interactions and nuclear organization with minimal false positives. We detail necessary steps for performing mammalian cell RBP-TRIBE to determine the targ
Externí odkaz:
https://doaj.org/article/69767ced513e4f8a96078c6dc0a9086c
Autor:
Dingbang Ma, Dariusz Przybylski, Katharine C Abruzzi, Matthias Schlichting, Qunlong Li, Xi Long, Michael Rosbash
Publikováno v:
eLife, Vol 10 (2021)
Many different functions are regulated by circadian rhythms, including those orchestrated by discrete clock neurons within animal brains. To comprehensively characterize and assign cell identity to the 75 pairs of Drosophila circadian neurons, we opt
Externí odkaz:
https://doaj.org/article/30565948e29e41ffaa9a492ecc5cd798
Publikováno v:
iScience, Vol 23, Iss 7, Pp 101318- (2020)
Summary: Both UV-cross-linking and immunoprecipitation (CLIP) and RNA editing (TRIBE) can identify the targets of RNA-binding proteins. To evaluate false-positives of CLIP and TRIBE, endogenous β-actin mRNA was tagged with MS2 stem loops, making it
Externí odkaz:
https://doaj.org/article/662bec2a6b224a6691b5bbe6e03fc32e
Autor:
Xiao Chen, Michael Rosbash
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
Accumulating evidence suggests that microRNAs play a role in circadian regulation. Here the authors show that in theDrosophila brain, mir-92a suppresses the excitability of PDF neurons—key circadian pacemaker cells in Drosophila—via inhibiting th
Externí odkaz:
https://doaj.org/article/489b58bf499f4508a9634b2675dbbd1d
Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity
Publikováno v:
eLife, Vol 8 (2019)
Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness with
Externí odkaz:
https://doaj.org/article/a7ff6fa3445b4ed1b1d536be98cf21f4
Publikováno v:
eLife, Vol 7 (2018)
Although alternative pre-mRNA splicing (AS) significantly diversifies the neuronal proteome, the extent of AS is still unknown due in part to the large number of diverse cell types in the brain. To address this complexity issue, we used an annotation
Externí odkaz:
https://doaj.org/article/9189de5f2ff0445886f559cc5546b678
Autor:
Katharine C Abruzzi, Abigail Zadina, Weifei Luo, Evelyn Wiyanto, Reazur Rahman, Fang Guo, Orie Shafer, Michael Rosbash
Publikováno v:
PLoS Genetics, Vol 13, Iss 2, p e1006613 (2017)
Locomotor activity rhythms are controlled by a network of ~150 circadian neurons within the adult Drosophila brain. They are subdivided based on their anatomical locations and properties. We profiled transcripts "around the clock" from three key grou
Externí odkaz:
https://doaj.org/article/3bae614a9ea7447d8078fac31026c506
Publikováno v:
eLife, Vol 5 (2016)
Activity-regulated genes (ARGs) are important for neuronal functions like long-term memory and are well-characterized in mammals but poorly studied in other model organisms like Drosophila. Here we stimulated fly neurons with different paradigms and
Externí odkaz:
https://doaj.org/article/cfc24e7d2de44a7faf353e4a575acb28
Autor:
Michael Rosbash
Publikováno v:
eLife, Vol 5 (2016)
The National Institutes of Health needs to make radical changes to ensure that biomedical research continues to thrive in the United States.
Externí odkaz:
https://doaj.org/article/17f976f564b242fda140679694e34333