Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Michael Rappleye"'
Autor:
Jason C. Klima, Lindsey A. Doyle, Justin Daho Lee, Michael Rappleye, Lauren A. Gagnon, Min Yen Lee, Emilia P. Barros, Anastassia A. Vorobieva, Jiayi Dou, Samantha Bremner, Jacob S. Quon, Cameron M. Chow, Lauren Carter, David L. Mack, Rommie E. Amaro, Joshua C. Vaughan, Andre Berndt, Barry L. Stoddard, David Baker
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
Fluorescent protein reporters based on GFP exist, but have intrinsic disadvantages. Here the authors incorporate pH, Ca2+ and protein–protein interaction sensing modalities into de novo designed mini-fluorescence-activating proteins (mFAPs), with i
Externí odkaz:
https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium Indicators
Real-time monitoring of biological activity can be achieved through the use of genetically encoded fluorescent indicators (GEFIs). GEFIs are protein-based sensing tools whose biophysical characteristics can be engineered to meet experimental needs. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::84c8b632c9f1ae26be82df33ec4805e6
https://doi.org/10.1101/2023.04.13.536801
https://doi.org/10.1101/2023.04.13.536801
Autor:
Michael Rappleye, Adam Gordon-Fennel, Daniel C. Castro, Avi K. Matarasso, Catalina A. Zamorano, Carrie Stine, Sarah J. Wait, Justin D. Lee, Jamison C. Siebart, Azra Suko, Netta Smith, Jeanot Muster, Kenneth A Matreyek, Douglas M. Fowler, Garrett D. Stuber, Michael R. Bruchas, Andre Berndt
Fluorescent sensor proteins are instrumental for detecting biological signals in vivo with high temporal accuracy and cell-type specificity. However, engineering sensors with physiological ligand sensitivity and selectivity is difficult because they
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91d9729e699ab5eb71bb17292594b3e2
https://doi.org/10.1101/2022.06.01.494241
https://doi.org/10.1101/2022.06.01.494241
Autor:
Samantha Bremner, Lindsey Doyle, Andre Berndt, Jason C. Klima, David L. Mack, Jiayi Dou, Lauren Carter, Justin Daho Lee, Emilia P. Barros, Joshua C. Vaughan, Cameron M. Chow, David Baker, Min Yen Lee, Michael Rappleye, Rommie E. Amaro, Jacob S. Quon, Lauren A. Gagnon, Anastassia A. Vorobieva, Barry L. Stoddard
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
Nature communications, vol 12, iss 1
Nature Communications
Nature communications, vol 12, iss 1
Nature Communications
Through the efforts of many groups, a wide range of fluorescent protein reporters and sensors based on green fluorescent protein and its relatives have been engineered in recent years. Here we explore the incorporation of sensing modalities into de n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fee693e5fb805cc50f72b2096a30e7f
https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
https://doaj.org/article/5f1531b0f2b14a3183076ec5a9a3c859
Autor:
Sihai Dave Zhao, Gregory L. Damhorst, Muhammad S. Khan, Manish Patel, Samuel Wachspress, Benjamin Davis, Rashid Bashir, Syed Anwaruddin, Michael Rappleye, Bobby Reddy, Sumeet Soni, Harsh Rawal, Ishan Taneja, Gillian Smith, Jackson Winter, Muhammad Ajmal, Zachary Price, Karen White, James Kumar, Tor W. Jensen, Umer Hassan, Jay Patel, Tanmay Ghonge, Ryan Healey, Raiya Sarwar, Ruoqing Zhu
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
Scientific Reports
Scientific Reports
Sepsis is a leading cause of death and is the most expensive condition to treat in U.S. hospitals. Despite targeted efforts to automate earlier detection of sepsis, current techniques rely exclusively on using either standard clinical data or novel b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ff08f8e446c042e7ec6fee4f9a48b70
http://link.springer.com/article/10.1038/s41598-017-09766-1
http://link.springer.com/article/10.1038/s41598-017-09766-1
Autor:
Gillian Smith, Manish Patel, Karen White, Sihai Dave Zhao, Michael Rappleye, Ryan Healey, Tanmay Ghonge, Ishan Taneja, Bobby Reddy, Umer Hassan, James Kumar, Sumeet Soni, Gregory L. Damhorst, Tor W. Jensen, Raiya Sarwar, Ruoqing Zhu, Samuel Wachspress, Jay Patel, Benjamin Davis, Muhammad Ajmal, Muhammad S. Khan, Syed Anwaruddin, Rashid Bashir, Zachary Price, Harsh Rawal, Jackson Winter
Publikováno v:
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-1 (2019)
Scientific Reports, Vol 9, Iss 1, Pp 1-1 (2019)
Sepsis is a leading cause of death and is the most expensive condition to treat in U.S. hospitals. Despite targeted efforts to automate earlier detection of sepsis, current techniques rely exclusively on using either standard clinical data or novel b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79957a0faaeffeec4eca8a29231afeea
http://europepmc.org/articles/PMC6864078
http://europepmc.org/articles/PMC6864078
Autor:
Alexander Hasnain, Umer Hassan, Michael Rappleye, Julia Liu, Zeeshan Haidry, Christine Lannon, Karen White, Ryan Healey, Jackson Winter, Tanmay Ghonge, Daniel Abboud, Na Teng Hung, Zachary Price, Naif Mansury, Nathaniel Leung, Enrique Valera, Jacob Berger, Rashid Bashir
Publikováno v:
Lab on a chip. 18(10)
Sepsis, an adverse auto-immune response to an infection often causing life-threatening complications, results in the highest mortality and treatment cost of any illness in US hospitals. Several immune biomarker levels, including Interleukin 6 (IL-6),
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a62f9877f88cd90b9d4052b20a820e08
https://pubmed.ncbi.nlm.nih.gov/29664086
https://pubmed.ncbi.nlm.nih.gov/29664086
Autor:
Ryan Healey, Tanmay Ghonge, Zachary Price, Isha Taneja, A. Tanna, Michael Rappleye, Benjamin Davis, Bobby Reddy, N. Manusry, Rashid Bashir, Karen White, Umer Hassan, Jacob Berger, Manish Patel, Siyao Liu, A. C. Hasnain, E. Flaugher, James Kumar, T. Jensen
Publikováno v:
Nature Communications
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
Sepsis, a potentially life-threatening complication of an infection, has the highest burden of death and medical expenses in hospitals worldwide. Leukocyte count and CD64 expression on neutrophils (nCD64) are known to correlate strongly with improved
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6607c1c0dc82243e1b17541c4b50a5f0
http://europepmc.org/articles/PMC5500847
http://europepmc.org/articles/PMC5500847