Investigation of the Influence of Charge State and Collision Energy on Oligonucleotide Fragmentation by Tandem Mass Spectrometry

Autor: Christopher Gawlig, Michael Rühl

Publikováno v: Molecules, Vol 28, Iss 3, p 1169 (2023)

Due to the increasing pharmaceutical interest of oligonucleotides, for example in antisense therapy and vaccines, their analytical characterization is of fundamental importance due to their complex structure. For this purpose, mass spectrometry is a

Externí odkaz: https://doaj.org/article/831bcd5296ef4b5faa216e14f035246e

Detergent-assisted sample preparation for MALDI-MS: Investigation of octylglucoside and docecylmaltoside for matrix crystallization, on-plate digestion, and trypsin activity

Autor: Michael Rühl, Michael Karas, Stefan Schönborn

Publikováno v: Journal of Mass Spectrometry. 53:675-679

We show an easy and fast method for improved detection of lipophilic peptides with MALDI-MS utilizing the nonionic detergents n-octylglucoside and n-dodecylmaltoside (laurylmaltoside). Investigations comprised on-plate digestion of proteins with tryp

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c75e7b68d782e4126e6e991ceaa836c
https://doi.org/10.1002/jms.4203

Characterization of the molecular mechanism of 5-lipoxygenase inhibition by 2-aminothiazoles

Autor: Carlo Angioni, Bettina Hofmann, Felix F Lillich, Manfred Schubert-Zsilavecz, Jessica Roos, Stefano Woltersdorf, Holger Stark, Michael Rühl, Mario Wurglics, Ann-Kathrin Häfner, Dieter Steinhilber, Simon B.M. Kretschmer, Dominik Vogt, Michael Karas, Astrid Kaiser, Gerd Geisslinger, Isabelle V. Maucher

Publikováno v: Biochemical Pharmacology. 123:52-62

5-Lipoxygenase (5-LO, EC1.13.11.34) has been implicated in the pathogenesis of inflammatory and immune diseases. Recently, aminothiazole comprising inhibitors have been discovered for this valuable target. Yet, the molecular mode of action of this cl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3e0eced889b420814620a56997b5620
https://doi.org/10.1016/j.bcp.2016.09.021

Nitro-fatty acids are formed in response to virus infection and are potent inhibitors of STING palmitoylation and signaling

Autor: Charlotte Möller, Gregory J. Buchan, Francisco J. Schopfer, Rachel E. Rigby, Hiroyuki Arai, Jonathan J. Miner, Wei Qian, Anne L. Thielke, Camilla Gunderstofte, Martin R. Jakobsen, Jan Rehwinkel, Jessica Roos, Mona Motwani, Sonia R. Salvatore, Katherine A. Fitzgerald, Marie B. Iversen, Rongtuan Lin, Raphaela Goldbach-Mansky, Christian K. Holm, Michael Rühl, Kojiro Mukai, Thorsten J. Maier, Tomohiko Taguchi, Cathrine A. Miner, Andreas S. Jakobsen, Anne-Louise S. Hansen, Emari Ogawa, David Olagnier, Sidsel D. Andersen

Publikováno v: Hansen, A L, Buchan, G J, Rühl, M, Mukai, K, Salvatore, S R, Ogawa, E, Andersen, S D, Iversen, M B, Thielke, A L, Gunderstofte, C, Motwani, M, Møller, C T, Jakobsen, A S, Fitzgerald, K A, Roos, J, Lin, R, Maier, T J, Goldbach-Mansky, R, Miner, C A, Qian, W, Miner, J J, Rigby, R E, Rehwinkel, J, Jakobsen, M R, Arai, H, Taguchi, T, Schopfer, F J, Olagnier, D & Holm, C K 2018, ' Nitro-fatty acids are formed in response to virus infection and are potent inhibitors of STING palmitoylation and signaling ', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 33, pp. E7768-E7775 . https://doi.org/10.1073/pnas.1806239115
Proceedings of the National Academy of Sciences of the United States of America

Significance Several chronic inflammatory conditions have recently been shown to depend on abnormally high activity of the signaling protein stimulator of IFN genes (STING). These conditions include examples from systemic lupus erythematosus, Aicardi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc7de5e98d88f9543f4ef4e8fec446d2
https://doi.org/10.1073/pnas.1806239115

Anti-inflammatory nitro-fatty acids suppress tumor growth by triggering mitochondrial dysfunction and activation of the intrinsic apoptotic pathway in colorectal cancer cells

Autor: Michael Rühl, Jasmin Fettel, Jennifer Grimmer, Benjamin Kühn, Anja Urbschat, Isabelle V. Maucher, Katharina J. Hock, Kai Zacharowski, Camilla Brat, Carmela Matrone, Thorsten J. Maier, Ufuk Bulut, Jessica Roos, Georg Manolikakes, Dieter Steinhilber, Alessa Kühn, Nadine Hellmuth

Publikováno v: Kühn, B, Brat, C, Fettel, J, Hellmuth, N, Maucher, I V, Bulut, U, Hock, K J, Grimmer, J, Manolikakes, G, Rühl, M, Kühn, A, Zacharowski, K, Matrone, C, Urbschat, A, Roos, J, Steinhilber, D & Maier, T J 2018, ' Anti-inflammatory nitro-fatty acids suppress tumor growth by triggering mitochondrial dysfunction and activation of the intrinsic apoptotic pathway in colorectal cancer cells ', Biochemical Pharmacology, vol. 155, pp. 48-60 . https://doi.org/10.1016/j.bcp.2018.06.014

Nitro-fatty acids (NFAs) are endogenously occurring lipid mediators exerting strong anti-inflammatory effects and acting as anti-oxidants in a number of animal models of inflammation. These NFA effects are mediated by targeting important regulatory p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::845771820333c091b10519b7790ab68e
https://pubmed.ncbi.nlm.nih.gov/29909078