Zobrazeno 1 - 10
of 37
pro vyhledávání: '"Michael P. Valley"'
Publikováno v:
Diabetes. 69
The interest in metabolic diseases like diabetes, nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH), continues to increase due to their growing impact on cell health. To facilitate studies and uncover novel drug targets
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8278e6667bcd12d7dc0c20d18663dffe
https://doi.org/10.2337/db20-2292-pub
https://doi.org/10.2337/db20-2292-pub
Autor:
Michael P. Valley, Coby B. Carlson, Jolanta Vidugiriene, James J. Cali, Natsuyo Aoyama, Natasha Karassina
Publikováno v:
Analytical Biochemistry. 505:43-50
Identifying activators and inhibitors of glucose uptake is critical for both diabetes management and anticancer therapy. To facilitate such studies, easy-to-use nonradioactive assays are desired. Here we describe a bioluminescent glucose uptake assay
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c7c80e61a9c172f87d26e62f8639666
https://doi.org/10.1016/j.ab.2016.04.010
https://doi.org/10.1016/j.ab.2016.04.010
Autor:
Donna Marie Leippe, Natasha Karassina, Michael P. Valley, Gediminas Vidugiris, James J. Cali, Jolanta Vidugiriene
Publikováno v:
Cancer Research. 79:5272-5272
The tumor microenvironment is complex, containing diverse cell populations with individual nutrient requirements. Tumor and immune cells must respond to the dysregulated and changing conditions of the tumor microenvironment, as well as compete for fu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d5176faf0d66243d61a85f3bad510aad
https://doi.org/10.1158/1538-7445.am2019-5272
https://doi.org/10.1158/1538-7445.am2019-5272
Publikováno v:
Cancer Research. 78:373-373
The growing interest in understanding metabolic adaptations that occur in cancer and immune cells in the tumor microenvironment and the advances made towards developing effective immunotherapies have increased the need for sensitive and efficient met
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f60e1b1d99a6967011ff9ad187123ea7
https://doi.org/10.1158/1538-7445.am2018-373
https://doi.org/10.1158/1538-7445.am2018-373
Publikováno v:
Bioorganic Chemistry. 38:115-119
The flavoenzyme nitroalkane oxidase catalyzes the oxidation of primary and secondary nitroalkanes to the corresponding aldehydes and ketones plus nitrite. The structure of the enzyme shows that Ser171 forms a hydrogen bond to the flavin N5, suggestin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22dde86ba5fbe167382304b7ced8ef7d
https://doi.org/10.1016/j.bioorg.2009.12.004
https://doi.org/10.1016/j.bioorg.2009.12.004
Autor:
Paul F. Fitzpatrick, Annie Heroux, Jiali Gao, Allen M. Orville, Michael P. Valley, Dan Thomas Major
Publikováno v:
Proceedings of the National Academy of Sciences. 106:20734-20739
The proton transfer reaction between the substrate nitroethane and Asp-402 catalyzed by nitroalkane oxidase and the uncatalyzed process in water have been investigated using a path-integral free-energy perturbation method. Although the dominating eff
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a96c3995f3bd154b45c834694f191515
https://doi.org/10.1073/pnas.0911416106
https://doi.org/10.1073/pnas.0911416106
Autor:
Annie Heroux, Dragana M. Bozinovski, Allen M. Orville, Paul F. Fitzpatrick, Michael P. Valley
Publikováno v:
Biochemistry. 48:3407-3416
The flavoenzyme nitroalkane oxidase is a member of the acyl-CoA dehydrogenase superfamily. Nitroalkane oxidase catalyzes the oxidation of neutral nitroalkanes to nitrite and the corresponding aldehydes or ketones. Crystal structures to 2.2 A resoluti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66a86a7fff68c499341a2f8407c5121d
https://doi.org/10.1021/bi8023042
https://doi.org/10.1021/bi8023042
Autor:
Wenhui Zhou, James J. Cali, Michael P. Valley, Keith V. Wood, Jianquan Liu, Robert F. Bulleit, Erika Hawkins, Christine Ann Andrews, John Shultz, Dieter Klaubert
Publikováno v:
ChemBioChem. 9:714-718
Alkaline phosphatase (AP)—a stable enzyme with high specific activity for the hydrolysis of phosphate esters—is widely used as a conjugated enzyme label in enzyme-linked immunosorbent assays (ELISA) and DNA hybridization assays. It is also used a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1fa9de0d4093d6afa45ad1d6ef47f93f
https://doi.org/10.1002/cbic.200700644
https://doi.org/10.1002/cbic.200700644
Autor:
Michael P. Valley, John Shultz, James J. Cali, Terry L. Riss, Martha O'brien, Andrew L. Niles
Publikováno v:
Expert Opinion on Drug Metabolism & Toxicology. 4:103-120
Bioluminescent assays couple a limiting component of a luciferase-catalyzed photon-emitting reaction to a variable parameter of interest, while holding the other components constant or non-limiting. In this way light output varies with the parameter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b4e381ec4ba7a8ee8bf1fca652e8e4c
https://doi.org/10.1517/17425255.4.1.103
https://doi.org/10.1517/17425255.4.1.103
Autor:
Allen M. Orville, Annie Heroux, Paul F. Fitzpatrick, Michael P. Valley, Dragana M. Bozinovski, Patrick G. Shaw
Publikováno v:
Biochemistry. 46:13800-13808
The flavoprotein nitroalkane oxidase (NAO) catalyzes the oxidation of primary and secondary nitroalkanes to the corresponding aldehydes and ketones. The enzyme is a homologue of acyl-CoA dehydrogenase. Asp402 in NAO has been proposed to be the active
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9258f24b5e6c37732ebeab2a4e89dc12
https://doi.org/10.1021/bi701557k
https://doi.org/10.1021/bi701557k