Zobrazeno 1 - 10
of 225
pro vyhledávání: '"Michael P, Rout"'
Autor:
Erin R. Butterfield, Samson O. Obado, Simon R. Scutts, Wenzhu Zhang, Brian T. Chait, Michael P. Rout, Mark C. Field
Publikováno v:
Nucleus, Vol 15, Iss 1 (2024)
ABSTRACTThe nuclear envelope (NE) separates translation and transcription and is the location of multiple functions, including chromatin organization and nucleocytoplasmic transport. The molecular basis for many of these functions have diverged betwe
Externí odkaz:
https://doaj.org/article/ae18073f1f5e4692a04948d564b2452b
Autor:
Natalia E Ketaren, Fred D Mast, Peter C Fridy, Jean Paul Olivier, Tanmoy Sanyal, Andrej Sali, Brian T Chait, Michael P Rout, John D Aitchison
Publikováno v:
eLife, Vol 12 (2024)
To date, all major modes of monoclonal antibody therapy targeting SARS-CoV-2 have lost significant efficacy against the latest circulating variants. As SARS-CoV-2 omicron sublineages account for over 90% of COVID-19 infections, evasion of immune resp
Externí odkaz:
https://doaj.org/article/235be37097d94a95a53877f57c2b8848
Autor:
Fred D Mast, Peter C Fridy, Natalia E Ketaren, Junjie Wang, Erica Y Jacobs, Jean Paul Olivier, Tanmoy Sanyal, Kelly R Molloy, Fabian Schmidt, Magdalena Rutkowska, Yiska Weisblum, Lucille M Rich, Elizabeth R Vanderwall, Nicholas Dambrauskas, Vladimir Vigdorovich, Sarah Keegan, Jacob B Jiler, Milana E Stein, Paul Dominic B Olinares, Louis Herlands, Theodora Hatziioannou, D Noah Sather, Jason S Debley, David Fenyö, Andrej Sali, Paul D Bieniasz, John D Aitchison, Brian T Chait, Michael P Rout
Publikováno v:
eLife, Vol 10 (2021)
The emergence of SARS-CoV-2 variants threatens current vaccines and therapeutic antibodies and urgently demands powerful new therapeutics that can resist viral escape. We therefore generated a large nanobody repertoire to saturate the distinct and hi
Externí odkaz:
https://doaj.org/article/81f21ae91fa645928a68302720f1052b
Autor:
Zhanna Hakhverdyan, Kelly R. Molloy, Roman I. Subbotin, Javier Fernandez-Martinez, Brian T. Chait, Michael P. Rout
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100800- (2021)
Summary: We present a comprehensive and robust protocol to track the dynamics of all proteins in a complex in yeast cells. A single member of the protein assembly is tagged and conditionally expressed, minimizing the perturbations to the protein comp
Externí odkaz:
https://doaj.org/article/8f14c84eb3e346c589f3e578b975f3cc
Autor:
Marc A Schureck, Joseph E Darling, Alan Merk, Jinfeng Shao, Geervani Daggupati, Prakash Srinivasan, Paul Dominic B Olinares, Michael P Rout, Brian T Chait, Kurt Wollenberg, Sriram Subramaniam, Sanjay A Desai
Publikováno v:
eLife, Vol 10 (2021)
Malaria parasites use the RhopH complex for erythrocyte invasion and channel-mediated nutrient uptake. As the member proteins are unique to Plasmodium spp., how they interact and traffic through subcellular sites to serve these essential functions is
Externí odkaz:
https://doaj.org/article/da19fed0a9f244e489c10d2cf3ae9a97
Publikováno v:
Trends Parasitol
Export of RNA from the nucleus is essential for all eukaryotic cells, with at least three major classes exported, mRNA, tRNA and rRNA. RNA export has emerged as a major step in the control of gene expression, with mRNA molecules required to complete
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61c1678c1057b4ddfc5ff356b67eebc8
https://doi.org/10.1016/j.pt.2022.07.008
https://doi.org/10.1016/j.pt.2022.07.008
Autor:
Mark C. Field, Michael P. Rout
Publikováno v:
F1000Research, Vol 8 (2019)
The name “eukaryote” is derived from Greek, meaning “true kernel”, and describes the domain of organisms whose cells have a nucleus. The nucleus is thus the defining feature of eukaryotes and distinguishes them from prokaryotes (Archaea and B
Externí odkaz:
https://doaj.org/article/e3a12e33ee52401d952c8be069527348
Autor:
Ryo Hayama, Mirco Sorci, John J Keating Iv, Lee M Hecht, Joel L Plawsky, Georges Belfort, Brian T Chait, Michael P Rout
Publikováno v:
PLoS ONE, Vol 14, Iss 6, p e0217897 (2019)
Protein-protein interactions are central to biological processes. In vitro methods to examine protein-protein interactions are generally categorized into two classes: in-solution and surface-based methods. Here, using the multivalent interactions bet
Externí odkaz:
https://doaj.org/article/66e918f3521149ff9b81ffa9d8eebd0a
Autor:
Toshiya Kozai, Javier Fernandez-Martinez, Trevor van Eeuwen, Paola Gallardo, Larisa E. Kapinos, Adam Mazur, Wenzhu Zhang, Jeremy Tempkin, Radhakrishnan Panatala, Maria Delgado-Izquierdo, Barak Raveh, Andrej Sali, Brian T. Chait, Liesbeth M. Veenhoff, Michael P. Rout, Roderick Y. H. Lim
Nuclear pore complexes (NPCs) mediate nucleocytoplasmic transport of specific macromolecules while impeding the exchange of unsolicited material. However, key aspects of this gating mechanism remain controversial. To address this issue, we determined
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c479bc774ff96fe6fb7c8fe212cc3b23
https://doi.org/10.1101/2023.03.31.535055
https://doi.org/10.1101/2023.03.31.535055
Autor:
Yael Udi, Wenzhu Zhang, Milana E. Stein, Inna Ricardo-Lax, Hilda A. Pasolli, Brian T. Chait, Michael P. Rout
Publikováno v:
Journal of Cell Biology. 222
Subcellular fractionation in combination with mass spectrometry–based proteomics is a powerful tool to study localization of key proteins in health and disease. Here we offered a reliable and rapid method for mammalian cell fractionation, tuned for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eaa0a6b0b2a9ba551e9830f532c64797
https://doi.org/10.1083/jcb.202209062
https://doi.org/10.1083/jcb.202209062