Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Michael P, Ledbetter"'
Autor:
Ramanarayanan Krishnamurthy, Koji Hashimoto, Emil C. Fischer, Rebekah J Karadeema, Yorke Zhang, Aaron W. Feldman, Floyd E. Romesberg, Ramkrishna Adhikary, Michael P. Ledbetter, Dien Vivian T
Publikováno v:
Nature chemical biology
Natural organisms use a four-letter genetic alphabet that makes available 64 triplet codons, of which 61 are sense codons used to encode proteins with the 20 canonical amino acids. We have shown that the unnatural nucleotides dNaM and dTPT3 can pair
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6550f70e588b59d3893309dbd2c33d9c
http://europepmc.org/articles/PMC7263176
http://europepmc.org/articles/PMC7263176
Autor:
Jens H. Gundlach, Matthew T. Noakes, Hwanhee C. Kim, Brooke A. Anderson, Jonathan M. Craig, Ramanarayanan Krishnamurthy, Jesse R. Huang, Rebekah J Karadeema, Michael P. Ledbetter, Sarah J. Abell, Floyd E. Romesberg
Publikováno v:
J Am Chem Soc
Unnatural base pairs (UBPs) have been developed and used for a variety of in vitro applications, as well as for the engineering of semi-synthetic organisms (SSOs) that store and retrieve increased information. However, these applications are limited
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0198d0b4025d8e82ca351a2a0986a6d4
https://europepmc.org/articles/PMC7263177/
https://europepmc.org/articles/PMC7263177/
Publikováno v:
Nature Chemical Biology. 14:361-367
As synthetic regulatory programs expand in sophistication, an ever increasing number of biological components with predictable phenotypes is required. Regulators are often ‘part mined’ from a diverse, but uncharacterized, array of genomic sequenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ff7e421acab25b2eca56113d4b391bf
https://doi.org/10.1038/s41589-018-0006-7
https://doi.org/10.1038/s41589-018-0006-7
Autor:
John C. Chaput, Michael P. Ledbetter, Floyd E. Romesberg, Emil C. Fischer, Jen-Yu Liao, Aaron W. Feldman
Publikováno v:
Journal of the American Chemical Society, vol 140, iss 4
Nucleoside triphosphates play a central role in biology, but efforts to study these roles have proven difficult because the levels of triphosphates are tightly regulated in a cell and because individual triphosphates can be difficult to label or modi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2157dba72578f3975e9ceb2c7eec6d50
https://doi.org/10.1021/jacs.7b11404
https://doi.org/10.1021/jacs.7b11404
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1973
The polymerase chain reaction (PCR) is a universal and essential tool in molecular biology and biotechnology, but it is generally limited to the amplification of DNA with the four-letter genetic alphabet. Here, we describe PCR amplification with a si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7460c05ec4b83de0be3b0bbad432df00
https://pubmed.ncbi.nlm.nih.gov/31016704
https://pubmed.ncbi.nlm.nih.gov/31016704
Publikováno v:
Methods in Molecular Biology ISBN: 9781493992157
The polymerase chain reaction (PCR) is a universal and essential tool in molecular biology and biotechnology, but it is generally limited to the amplification of DNA with the four-letter genetic alphabet. Here, we describe PCR amplification with a si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7ace3233cb91c31c00bd2fb9d523097
https://doi.org/10.1007/978-1-4939-9216-4_13
https://doi.org/10.1007/978-1-4939-9216-4_13
Publikováno v:
Current opinion in chemical biology. 46
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c1ce364eae1468be5038f9d7e927336
https://pubmed.ncbi.nlm.nih.gov/30270151
https://pubmed.ncbi.nlm.nih.gov/30270151
Semi-synthetic organisms (SSOs) created from Escherichia coli can replicate a plasmid containing an unnatural base pair (UBP) formed between the synthetic nucleosides dNaM and dTPT3 (dNaM-dTPT3) when the corresponding unnatural triphosphates are impo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e04f1918155659ff36b52cd750fef223
https://europepmc.org/articles/PMC5793209/
https://europepmc.org/articles/PMC5793209/
Publikováno v:
Biochemistry and Molecular Biology Education. 41:433-442
Evolution is a defining criterion of life and is central to understanding biological systems. However, the timescale of evolutionary shifts in phenotype limits most classroom evolution experiments to simple probability simulations. In vitro directed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::05b33baa2565bb6b7a9976972efce333
https://doi.org/10.1002/bmb.20742
https://doi.org/10.1002/bmb.20742
Publikováno v:
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology. 41(6)
Evolution is a defining criterion of life and is central to understanding biological systems. However, the timescale of evolutionary shifts in phenotype limits most classroom evolution experiments to simple probability simulations. In vitro directed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b2c3b9029b55e5f390b60b2430737529
https://pubmed.ncbi.nlm.nih.gov/24214216
https://pubmed.ncbi.nlm.nih.gov/24214216