Zobrazeno 1 - 10
of 79
pro vyhledávání: '"Michael N. Berry"'
Publikováno v:
BMJ Case Reports. 14:e242658
A healthy 27-year-old primigravida of middle eastern ethnicity presented for prenatal care after arriving in Canada in the early second trimester. Anatomy scan at 20 weeks was normal, and gestational diabetes was diagnosed on routine screening. Glyca
Publikováno v:
Liver International. 23:201-211
Isolated hepatocytes in suspension provide a number of advantages for use in bioartificial liver device, however, poor stability of this cell preparation at physiological temperatures is an apparent barrier preventing their use. We therefore investig
Publikováno v:
Journal of Theoretical Biology. 217:509-523
We have used a random walk model of glycolysis and gluconeogenesis to investigate the bioenergetic implications of considering the cell cytoplasm to be a uniform well-mixed compartment. Radiotracer studies conducted on hepatocytes harvested from fast
Publikováno v:
European Journal of Biochemistry. 269:792-797
Glycolysis from [6-(3)H]glucose and gluconeogenesis from [U-(14)C]glycerol were examined in isolated hepatocytes from fasted rats. A 5 mm bolus of glycerol inhibited phosphorylation of 40 mm glucose by 50% and glycolysis by more than 60%, and caused
Publikováno v:
Biochemical Journal. 337:497-501
The stimulation of glucose phosphorylation in isolated hepatocytes by low fructose concentrations is transient due to the rapid metabolism of fructose. To prolong this stimulatory effect fructose was enzymically generated in the incubation medium fro
Autor:
Mary-Ann Efthivoulou, Michael N. Berry
Publikováno v:
Alcoholism: Clinical and Experimental Research. 21:267-274
The addition of antipyrine or aminopyrine to isolated hepatocytes derived from normal rats and incubated with ethanol caused a significant decrease in the oxidation of ethanol to acetate. This decrease was associated with a corresponding accumulation
Publikováno v:
Yeast. 13:119-125
Intracellular metabolic flux has been investigated in two strains of Saccharomyces cerevisiae grown into stationary phase under both glucose-repressed and glucose-derepressed conditions. By employing a variety of simple methodologies (manometry, enzy
Publikováno v:
Cell Biology and Toxicology. 13:223-233
The first technique for large-scale preparation of isolated hepatocytes was described in 1953 and involved perfusion of rat liver under pressure with a Ca2+-free solution containing a chelating agent. Various modifications of this technique were in u
Autor:
Mary-Ann Efthivoulou, Michael N. Berry
Publikováno v:
Biochemical Pharmacology. 52:863-867
The addition of aniline to isolated hepatocytes derived from fasted rats and incubated with ethanol, caused a 30-60% decrease in the rate of ethanol oxidation. The degree of inhibition was dependent on aniline concentration, 5 mM causing near-maximal
Autor:
Anthony R. Grivell, Michael N. Berry
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1291:83-88
The influence of phosphate-free medium buffered with synthetic organic buffers, and of a preliminary incubation of cells in medium lacking added substrate ('pre-incubation') was investigated with mouse-cultured Ehrlich ascites tumour cells. In compar