Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Michael L Nonet"'
Autor:
Ronit Marom, Bo Zhang, Megan E Washington, I-Wen Song, Lindsay C Burrage, Vittoria C Rossi, Ava S Berrier, Anika Lindsey, Jacob Lesinski, Michael L Nonet, Jian Chen, Dustin Baldridge, Gary A Silverman, V Reid Sutton, Jill A Rosenfeld, Alyssa A Tran, M John Hicks, David R Murdock, Hongzheng Dai, MaryAnn Weis, Shalini N Jhangiani, Donna M Muzny, Richard A Gibbs, Richard Caswell, Carrie Pottinger, Deirdre Cilliers, Karen Stals, Undiagnosed Diseases Network, David Eyre, Deborah Krakow, Tim Schedl, Stephen C Pak, Brendan H Lee
Publikováno v:
PLoS Genetics, Vol 19, Iss 11, p e1011005 (2023)
BackgroundKinesin motor proteins transport intracellular cargo, including mRNA, proteins, and organelles. Pathogenic variants in kinesin-related genes have been implicated in neurodevelopmental disorders and skeletal dysplasias. We identified de novo
Externí odkaz:
https://doaj.org/article/d6d02fb43ddb4cad86147275323bfe06
Autor:
Qun Zheng, Shikha Ahlawat, Anneliese Schaefer, Tim Mahoney, Sandhya P Koushika, Michael L Nonet
Publikováno v:
PLoS Genetics, Vol 10, Iss 10, p e1004644 (2014)
Axonal transport of synaptic vesicles (SVs) is a KIF1A/UNC-104 mediated process critical for synapse development and maintenance yet little is known of how SV transport is regulated. Using C. elegans as an in vivo model, we identified SAM-4 as a nove
Externí odkaz:
https://doaj.org/article/a6c1e199ecf04b3fab9351a3c2a888f2
Publikováno v:
eLife, Vol 3 (2014)
Microtubule dynamics facilitate neurite growth and establish morphology, but the role of minus-end binding proteins in these processes is largely unexplored. CAMSAP homologs associate with microtubule minus-ends, and are important for the stability o
Externí odkaz:
https://doaj.org/article/cd48dda5a2364b238df398c5d218c5ab
Autor:
Jitendra Kumar, Bikash C Choudhary, Raghu Metpally, Qun Zheng, Michael L Nonet, Sowdhamini Ramanathan, Dieter R Klopfenstein, Sandhya P Koushika
Publikováno v:
PLoS Genetics, Vol 6, Iss 11, p e1001200 (2010)
UNC-104/KIF1A is a Kinesin-3 motor that transports synaptic vesicles from the cell body towards the synapse by binding to PI(4,5)P(2) through its PH domain. The fate of the motor upon reaching the synapse is not known. We found that wild-type UNC-104
Externí odkaz:
https://doaj.org/article/c64ab57b978c4b05bca8d37e296f8082
Publikováno v:
PLoS ONE, Vol 5, Iss 4, p e10161 (2010)
Antibodies are critical tools in many avenues of biological research. Though antibodies can be produced in the research laboratory setting, most research labs working with vertebrates avail themselves of the wide array of commercially available reage
Externí odkaz:
https://doaj.org/article/7e83a430cb0d4dd2b28cd9dcc98ed316
Autor:
Elena O Gracheva, Anna O Burdina, Andrea M Holgado, Martine Berthelot-Grosjean, Brian D Ackley, Gayla Hadwiger, Michael L Nonet, Robby M Weimer, Janet E Richmond
Publikováno v:
PLoS Biology, Vol 4, Iss 8, p e261 (2006)
Caenorhabditis elegans TOM-1 is orthologous to vertebrate tomosyn, a cytosolic syntaxin-binding protein implicated in the modulation of both constitutive and regulated exocytosis. To investigate how TOM-1 regulates exocytosis of synaptic vesicles in
Externí odkaz:
https://doaj.org/article/a9bfb76e606a4a628fa2d5c0947a4b42
Autor:
Michael L Nonet
Publikováno v:
GENETICS.
I outline a streamlined method to insert large, single-copy transgenes into the Caenorhabditis elegans genome using recombination-mediated cassette exchange (RMCE) that relies solely on drug selection yielding a homozygous fluorescent protein (FP) ma
Autor:
Michael L. Nonet
I outline a streamlined method to create single copy large genomic insert transgenes using Recombination-Mediated Cassette Exchange (RMCE) that relies solely on drug selection yielding a homozygous fluorescent protein (FP) marked transgene in 3 gener
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::82efe959a45e9571525cf7cab5e618e2
https://doi.org/10.1101/2023.03.05.531207
https://doi.org/10.1101/2023.03.05.531207
Autor:
Michael L. Nonet
Publikováno v:
Genetics. 215:903-921
The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach
Autor:
Michael L, Nonet
Publikováno v:
Genetics
The application of CRISPR technology has greatly facilitated the creation of transgenic Caenorhabditis elegans lines. However, methods to insert multi-kilobase DNA constructs remain laborious even with these advances. Here, I describe a new approach