Zobrazeno 1 - 10
of 70
pro vyhledávání: '"Michael Kovermann"'
Autor:
Karin Vogel, Tobias Bläske, Marie-Kristin Nagel, Christoph Globisch, Shane Maguire, Lorenz Mattes, Christian Gude, Michael Kovermann, Karin Hauser, Christine Peter, Erika Isono
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-19 (2022)
The endocytic degradation of plasma membrane proteins can be modulated by deubiquitylating enzymes (DUBs). Here, the authors describe two plasma membrane localized Arabidopsis DUBs that can be activated by binding to anionic lipids and influence the
Externí odkaz:
https://doaj.org/article/e31b4e70945b43d6a26752c15f71c864
Autor:
Simon Maria Kienle, Tobias Schneider, Katrin Stuber, Christoph Globisch, Jasmin Jansen, Florian Stengel, Christine Peter, Andreas Marx, Michael Kovermann, Martin Scheffner
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
Ubiquitin is not only a posttranslational modifier but itself is subject to modifications, such as acetylation. Characterization of distinct acetylated ubiquitin variants reveals that each acetylation site has a particular impact on ubiquitin structu
Externí odkaz:
https://doaj.org/article/1e21cc4548474d48816030ee2117176a
Autor:
Katrin Stuber, Tobias Schneider, Jill Werner, Michael Kovermann, Andreas Marx, Martin Scheffner
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Both ubiquitin and NEDD8 can be phosphorylated, but the biological role of NEDD8 phosphorylation remains unclear. Here, the authors identify similarities and differences of ubiquitin and NEDD8 phosphorylation, showing that phosphorylated NEDD8 has a
Externí odkaz:
https://doaj.org/article/372e70c5bc914032a83674ddead0eef0
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Abstract The concept of Molecular Crowding depicts the high density of diverse molecules present in the cellular interior. Here, we determine the impact of low molecular weight and larger molecules on binding capacity of single-stranded DNA (ssDNA) t
Externí odkaz:
https://doaj.org/article/8a50fd7795844c2d80b2f7d036f8616f
Autor:
Birgit Köhn, Michael Kovermann
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
The precise effects of crowding on protein folding have been difficult to establish. Here the authors apply multidimensional high-resolution NMR spectroscopy to provide insight on the local impact of macromolecular crowding on the thermodynamic stabi
Externí odkaz:
https://doaj.org/article/bc473e3879294d3695303fbc031fcd16
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0215411 (2019)
We report here on the stability and catalytic properties of the HoLaMa DNA polymerase, a Klenow sub-fragment lacking the 3'-5' exonuclease domain. HoLaMa was overexpressed in Escherichia coli, and the enzyme was purified by means of standard chromato
Externí odkaz:
https://doaj.org/article/cf506e5f50cd4f31b3cfc778871564e0
Publikováno v:
PLoS ONE, Vol 11, Iss 9, p e0163115 (2016)
Enzymes have evolved with highly specific values of their catalytic parameters kcat and KM. This poses fundamental biological questions about the selection pressures responsible for evolutionary tuning of these parameters. Here we are address these q
Externí odkaz:
https://doaj.org/article/f76a02e2b551409cb70c635a7105d551
Autor:
Andreas Hoffmann, Michael Kovermann, Hauke Lilie, Markus Fiedler, Jochen Balbach, Rainer Rudolph, Sven Pfeifer
Publikováno v:
PLoS ONE, Vol 7, Iss 2, p e31298 (2012)
A variety of approaches have been employed to generate binding proteins from non-antibody scaffolds. Utilizing a beta-sheet of the human ubiquitin for paratope creation we obtained binding proteins against tumor necrosis factor (TNF)-alpha. The bioac
Externí odkaz:
https://doaj.org/article/ca311b7ba68449ba8cee891da8540427
Publikováno v:
PLoS ONE, Vol 7, Iss 6, p e38244 (2012)
Progress in functional and structural studies of integral membrane proteins (IMPs) is lacking behind their soluble counterparts due to the great challenge in producing stable and homogeneous IMPs. Low natural abundance, toxicity when over-expressed a
Externí odkaz:
https://doaj.org/article/36a3d428bd464a8ca44d69aa969aca32
Autor:
Michael Kovermann, Alessandra Stefan, Chiara Palazzetti, Fabian Immler, Fabrizio Dal Piaz, Luca Bernardi, Valentina Cimone, Maria Laura Bellone, Alejandro Hochkoeppler
Publikováno v:
Biochimie. 213:66-81