Zobrazeno 1 - 10
of 281
pro vyhledávání: '"Michael K, Jensen"'
Autor:
Søren D Petersen, Lucas Levassor, Christine M Pedersen, Jan Madsen, Lea G Hansen, Jie Zhang, Ahmad K Haidar, Rasmus J N Frandsen, Jay D Keasling, Tilmann Weber, Nikolaus Sonnenschein, Michael K Jensen
Publikováno v:
PLoS Computational Biology, Vol 20, Iss 3, p e1011929 (2024)
Synthetic biology dictates the data-driven engineering of biocatalysis, cellular functions, and organism behavior. Integral to synthetic biology is the aspiration to efficiently find, access, interoperate, and reuse high-quality data on genotype-phen
Externí odkaz:
https://doaj.org/article/c2a8272c0f8f4e8583e467f28cf7f764
Autor:
Emil D. Jensen, Marcus Deichmann, Xin Ma, Rikke U. Vilandt, Giovanni Schiesaro, Marie B. Rojek, Bettina Lengger, Line Eliasson, Justin M. Vento, Deniz Durmusoglu, Sandie P. Hovmand, Ibrahim Al’Abri, Jie Zhang, Nathan Crook, Michael K. Jensen
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
G protein-coupled receptors (GPCRs) enable cells to sense environmental cues and are indispensable for coordinating vital processes including quorum sensing, proliferation, and sexual reproduction. Here the authors, using heterologous GPCR expression
Externí odkaz:
https://doaj.org/article/47f9576f187340baa92bf1ba45054259
Autor:
Vasil D'Ambrosio, Lea G. Hansen, Jie Zhang, Emil D. Jensen, Dushica Arsovska, Marcos Laloux, Tadas Jakočiūnas, Pernille Hjort, Davide De Lucrezia, Serena Marletta, Jay D. Keasling, Michael K. Jensen
Publikováno v:
Synthetic and Systems Biotechnology, Vol 7, Iss 2, Pp 657-663 (2022)
The synthetic biology toolkit for baker's yeast, Saccharomyces cerevisiae, includes extensive genome engineering toolkits and parts repositories. However, with the increasing complexity of engineering tasks and versatile applications of this model eu
Externí odkaz:
https://doaj.org/article/350328b816f64150aa8b75abe0b35547
Autor:
Emil D. Jensen, Francesca Ambri, Marie B. Bendtsen, Alex A. Javanpour, Chang C. Liu, Michael K. Jensen, Jay D. Keasling
Publikováno v:
Microbial Biotechnology, Vol 14, Iss 6, Pp 2617-2626 (2021)
Summary Directed evolution is a powerful method to optimize proteins and metabolic reactions towards user‐defined goals. It usually involves subjecting genes or pathways to iterative rounds of mutagenesis, selection and amplification. While powerfu
Externí odkaz:
https://doaj.org/article/2dcfe0c268f74d13888f38a9b161fae9
Autor:
Jie Zhang, Søren D. Petersen, Tijana Radivojevic, Andrés Ramirez, Andrés Pérez-Manríquez, Eduardo Abeliuk, Benjamín J. Sánchez, Zak Costello, Yu Chen, Michael J. Fero, Hector Garcia Martin, Jens Nielsen, Jay D. Keasling, Michael K. Jensen
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
In metabolic engineering, mechanistic models require prior metabolism knowledge of the chassis strain, whereas machine learning models need ample training data. Here, the authors combine the mechanistic and machine learning models to improve predicti
Externí odkaz:
https://doaj.org/article/12b7d69682c04fc38cfe252ad2aedc1b
Autor:
Tadas Jakočiūnas, Andreas K. Klitgaard, Eftychia Eva Kontou, Julie Bang Nielsen, Emil Thomsen, David Romero-Suarez, Kai Blin, Christopher J. Petzold, Jennifer W. Gin, Yaojun Tong, Charlotte Held Gotfredsen, Pep Charusanti, Rasmus J.N. Frandsen, Tilmann Weber, Sang Yup Lee, Michael K. Jensen, Jay D. Keasling
Publikováno v:
Synthetic and Systems Biotechnology, Vol 5, Iss 1, Pp 11-18 (2020)
To accelerate the shift to bio-based production and overcome complicated functional implementation of natural and artificial biosynthetic pathways to industry relevant organisms, development of new, versatile, bio-based production platforms is requir
Externí odkaz:
https://doaj.org/article/e0f913ee7f3644809c9f5660c13077d9
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Plants produce some of the most potent therapeutics and have been used for thousands of years to treat human diseases. Today, many medicinal natural products are still extracted from source plants at scale as their complexity precludes total synthesi
Externí odkaz:
https://doaj.org/article/055b6990f364454f9c678d756fdced61
Autor:
Hao Luo, Anne Sofie L Hansen, Lei Yang, Konstantin Schneider, Mette Kristensen, Ulla Christensen, Hanne B Christensen, Bin Du, Emre Özdemir, Adam M Feist, Jay D Keasling, Michael K Jensen, Markus J Herrgård, Bernhard O Palsson
Publikováno v:
PLoS Biology, Vol 17, Iss 3, p e2007050 (2019)
We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of ano
Externí odkaz:
https://doaj.org/article/8a014f6bc453477b97b3c4f4f1ca61cc
Autor:
Helén Olsson, Paul Cachera, Hilde Coumou, Mads L. Jensen, Benjamín J. Sánchez, Tomas Strucko, Marcel van den Broek, Jean-Marc Daran, Michael K. Jensen, Nikolaus Sonnenschein, Michael Lisby, Uffe H. Mortensen
Biological functions are orchestrated by intricate networks of interacting genetic elements. Predicting the interaction landscape remains a challenge for systems biology and the identification of phenotypic maximas would be of great benefit to synthe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::993ef7c9b9d3674f40a99fd4cf6d6ef9
https://doi.org/10.1101/2022.07.19.500587
https://doi.org/10.1101/2022.07.19.500587
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2513
Here, in this chapter, we describe a detailed protocol for the method named Cas9-mediated protein evolution reaction or short CasPER. CasPER is based on the generation of large 300-600-bp mutagenized linear DNA fragments by error-prone PCR which are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::62e00b00a4a7a61a5a121106a68403b5
https://pubmed.ncbi.nlm.nih.gov/35781198
https://pubmed.ncbi.nlm.nih.gov/35781198