Zobrazeno 1 - 10
of 226
pro vyhledávání: '"Michael J. Sutcliffe"'
Publikováno v:
Scopus-Elsevier
The structure of the 129-residue protein hen lysozyme has been determined in solution by two-dimensional 1H nuclear magnetic resonance methods. 1158 NOE distance restraints, and 68 phi and 24 chi 1 dihedral angle restraints were employed in conjuncti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b2a475ce7e3afaea5a405a5c8226c1e
https://doi.org/10.1006/jmbi.1993.1097
https://doi.org/10.1006/jmbi.1993.1097
Publikováno v:
Jones, S, Sutcliffe, M, Harris, D & Bragg, J 2016, ' To what extent is capital expenditure in UK Higher Education meeting the pedagogical needs of staff and students? ', Journal of Higher Education Policy and Management, vol. 38, no. 4 . https://doi.org/10.1080/1360080X.2016.1181881, https://doi.org/10.1080/1360080X.2016.1181881
Capital expenditure at United Kingdom (UK) universities is rapidly rising, with new buildings erected on the premise that national and international competitiveness must be maintained. We examine students’ engagement with and conceptualisation of u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36e5544a663c584ea5593fe084283c9a
https://eprints.kingston.ac.uk/id/eprint/35452/1/Sutcliffe-M-35452-AAM.pdf
https://eprints.kingston.ac.uk/id/eprint/35452/1/Sutcliffe-M-35452-AAM.pdf
Publikováno v:
Journal of the American Chemical Society. 134:9749-9754
The rate and kinetic isotope effect (KIE) on proton transfer during the aromatic amine dehydrogenase-catalyzed reaction with phenylethylamine shows complex pressure and temperature dependences. We are able to rationalize these effects within an envir
Autor:
Ekaterina Ivanova, Michael J. Sutcliffe, Hui Lu, Jiayun Pang, Jim Warwicker, Guanhua Yan, Thomas A. Jowitt
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 80:602-615
Protein-protein interaction is a fundamental process in all major biological processes. The hexameric Tim9-Tim10 (translocase of inner membrane) complex of the mitochondrial intermembrane space plays an essential chaperone-like role during import of
Publikováno v:
Angewandte Chemie. 123:2177-2180
Autor:
Linus O. Johannissen, Nigel S. Scrutton, Christopher R. Pudney, Michael J. Sutcliffe, Sam Hay
Publikováno v:
Journal of the American Chemical Society. 132:11329-11335
The role of dynamical effects in enzyme catalysis is both complex and widely debated. Understanding how dynamics can influence the barrier to an enzyme catalyzed reaction requires the development of new methodologies and tools. In particular compress
Publikováno v:
Biochemical Society Transactions. 38:875-878
In the present article, we describe the two standard high-throughput methods for identification of protein complexes: two-hybrid screens and TAP (tandem affinity purification) tagging. These methods have been used to characterize the interactome of S
Publikováno v:
Journal of Physical Organic Chemistry. 23:696-701
We report the first study of the effects of hydrostatic pressure on α-2° KIEs for an enzyme-catalysed H-transfer reaction that occurs by ‘deep’ tunnelling. High pressure causes a significant decrease in the observed α-2° KIE on the pre-steady
Publikováno v:
Biophysical Journal. 98(1):121-128
It is generally accepted that enzymes catalyze reactions by lowering the apparent activation energy by transition state stabilization or through destabilization of ground states. A more controversial proposal is that enzymes can also accelerate react
Autor:
Jianfeng Feng, Dimitris Vavoulis, Michael J. Sutcliffe, Peter R. Stanfield, I. Ashmole, Phillip J. Stansfeld, Puja Mehta
Publikováno v:
The Journal of Physiology. 587:4769-4783
Although the tandem pore potassium channel TASK-3 is thought to open and shut at its selectivity filter in response to changes of extracellular pH, it is currently unknown whether the channel also shows gating at its inner, cytoplasmic mouth through