Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Michael J. Kosinski"'
Autor:
Jon E. Hess, Thomas A. Delomas, Aaron D. Jackson, Michael J. Kosinski, Mary L. Moser, Laurie L. Porter, Greg Silver, Tod Sween, Laurie A. Weitkamp, Shawn R. Narum
Publikováno v:
Transactions of the American Fisheries Society. 151:263-296
Publikováno v:
Applied microbiology and biotechnology. 37(3)
Because induction of proteolytic activity and stress-response proteins can significantly affect expression levels in recombinant Escherichia coli, the influence of low-level expression of a mutant beta-galactosidase was investigated. A single copy of
Autor:
James E. Bailey, Michael J. Kosinski
Publikováno v:
Journal of biotechnology. 23(2)
Structural properties of two similar beta-galactosidase fragments were investigated to determine how they influence the fragments' degradation rate in Escherichia coli. Both fragments resulting from a C-terminal nonsense mutation in lacZ, the CSH11 p
Publikováno v:
Applied Microbiology and Biotechnology. 36
Addition of isopropyl β-D-thiogalactopyranoside (IPTG) to a strain of Escherichia coli with one lac promoter in its chromosome causes reduction in synthesis rate for a set of protiens. One of these proteins, designated H35, is a prominent cellular p