A687V EZH2 Is a Driver of Histone H3 Lysine 27 (H3K27) Hypertrimethylation

Autor: Ryan G. Kruger, Yuchen Bai, Yong Jiang, Caretha L. Creasy, Heidi M. Ott, Melissa B. Pappalardi, Edward Dul, Dashyant Dhanak, Michael T. McCabe, Benjamin Schwartz, Roland S. Annan, Peter J. Tummino, Wendy S. Halsey, Arthur Groy, Alan P. Graves, Steven D. Knight, Ashley M. Hughes, Michael J. Huddleston, Sharad K. Verma

Publikováno v: Molecular Cancer Therapeutics. 13:3062-3073

The EZH2 methyltransferase silences gene expression through methylation of histone H3 on lysine 27 (H3K27). Recently, EZH2 mutations have been reported at Y641, A677, and A687 in non-Hodgkin lymphoma. Although the Y641F/N/S/H/C and A677G mutations ex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4db6bf71136391841e89a91fdd651521
https://doi.org/10.1158/1535-7163.mct-13-0876

An optimized platform for hydrophilic interaction chromatography-immobilized metal affinity chromatography enables deep coverage of the rat liver phosphoproteome

Autor: Roland S. Annan, Francesca Zappacosta, Gilbert F. Scott, Michael J. Huddleston

Publikováno v: Journal of proteome research. 14(2)

While analysis of the phosphoproteome has become an important component of understanding how cells function, it remains a nontrivial task in terms of the number of sample preparation steps and instrument time needed to achieve sufficient depth of cov

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e96817b5c9f92ea3238a31955f34e77
https://pubmed.ncbi.nlm.nih.gov/25575281

Mistranslational errors associated with the rare arginine codon CGG in Escherichia coli

Autor: Dean E, McNulty, Brett A, Claffee, Michael J, Huddleston, M Lauren, Porter, Katie M, Cavnar, James F, Kane

Publikováno v: Protein Expression and Purification. 27:365-374

In Escherichia coli , CGG is a rare arginine codon occurring at a frequency of 0.54% in all E. coli mRNAs or 9.8% when an arginine residue is encoded for. When present in high numbers or in clusters in highly expressed recombinant mRNA, rare codons c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ce834b8e596b1d32966d5621493526f
https://doi.org/10.1016/s1046-5928(02)00610-1

SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer

Autor: Michiel Vermeulen, Nicolas Reynoird, Julien Sage, Olena Barbash, Or Gozani, Atul J. Butte, Shichong Liu, Michael J. Huddleston, Ryan G. Kruger, Dashyant Dhanak, Purvesh Khatri, Alex W. Wilkinson, Pascal W. T. C. Jansen, Benjamin A. Garcia, Pawel K. Mazur, Peter J. Tummino, Glenn S. Van Aller

Publikováno v: Nature
Nature, Nature Publishing Group, 2014, 510 (7504), pp.283-287. ⟨10.1038/nature13320⟩
Nature, 510, 283-7
Nature, 510, 7504, pp. 283-7

Deregulation of lysine methylation signalling has emerged as a common aetiological factor in cancer pathogenesis, with inhibitors of several histone lysine methyltransferases (KMTs) being developed as chemotherapeutics. The largely cytoplasmic KMT SM

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5b53225d89fe016f3889175b6d8d1e0
https://hal.archives-ouvertes.fr/hal-02989816

Mechanism of Inhibition of Cathepsin K by Potent, Selective 1,5-Diacylcarbohydrazides: A New Class of Mechanism-Based Inhibitors of Thiol Proteases

Autor: David G. Tew, Mark Alan Levy, Thaddeus A. Tomaszek, Thomas D. Meek, Daniel F. Veber, Stacie Halpert, Michael J. Huddleston, Steven A. Carr, Carl F. Ijames, Jacques Briand, Scott K. Thompson, Mary J. Bossard

Publikováno v: Biochemistry. 38:15893-15902

The nature of the inhibition of thiol proteases by a new class of mechanism-based inhibitors, 1,5-diacylcarbohydrazides, is described. These potent, time-dependent, active-site spanning inhibitors include compounds that are selective for cathepsin K,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::7f53be335bf0b10592b2e03ed037fa0f
https://doi.org/10.1021/bi991193+