Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Michael J. Feldhaus"'
Autor:
Ulrik B. Nielsen, Birgit Schoeberl, Arthur J. Kudla, Michael J. Feldhaus, Matt Wallace, Neeraj Kohli, Jinming Gu, Stephanie Nguyen, Ryan Overland, Lia Luus, Bo Zhang, Shinji Oyama, Violette Paragas, Sharlene Adams, Brian D. Harms, Alexandra Huhalov, Charlotte F. McDonagh
PDF file - 189K
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::740c3b57b3148fec19b2a1932a1c942b
https://doi.org/10.1158/1535-7163.22498605
https://doi.org/10.1158/1535-7163.22498605
Autor:
James D. Marks, John R. Barr, Sean A. Gray, Suzanne R. Kalb, Keith D. Miller, Cheryl L. Baird, Gerard A. Cangelosi, Michael J. Feldhaus
Publikováno v:
Biotechnology and Bioengineering. 108:2456-2467
A non-immune library of human single chain fragment variable (scFv) antibodies displayed on Sacchar- omyces cerevisiae was screened for binding to the Clostridium botulinum neurotoxin serotype A binding domain (BoNT/A (Hc)) with the goal of identifyi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::44c964a840188d8f3028eaeeeda30463
https://doi.org/10.1002/bit.23196
https://doi.org/10.1002/bit.23196
Publikováno v:
Protein Engineering, Design and Selection. 18:527-536
Yeast display is a powerful tool for increasing the affinity and thermal stability of scFv antibodies through directed evolution. Mammalian calmodulin (CaM) is a highly conserved signaling protein that undergoes structural changes upon Ca 2 + binding
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4adc36ed108b180135b00ede31db9b19
https://doi.org/10.1093/protein/gzi060
https://doi.org/10.1093/protein/gzi060
Autor:
James D. Marks, Thomas J. Smith, Jianlong Lou, Robert W. Siegel, Consuelo Garcia-Rodriguez, Isin N. Geren, R. Tsai, C.M. Forsyth, Leonard A. Smith, Y. Robles, Ali Razai, Michael J. Feldhaus
Publikováno v:
Journal of Molecular Biology. 351:158-169
Botulism is caused by botulinum neurotoxin (BoNT), the most poisonous substance known. Potential use of BoNT as a biothreat agent has made development of sensitive assays for toxin detection and potent antitoxin for treatment of intoxication a high p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d325a3f0805bde18bd15f65fa7019cc4
https://doi.org/10.1016/j.jmb.2005.06.003
https://doi.org/10.1016/j.jmb.2005.06.003
Autor:
Robert W. Siegel, Michael J. Feldhaus
Publikováno v:
Journal of Immunological Methods. 290:69-80
Yeast display of antibody fragments has proven to be an efficient and productive means for directed evolution of single-chain Fv (scFv) antibodies for increased affinity and thermal stability and, more recently, for the display and screening of a non
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a7e04cfb8c2abef1f5969cf8845ebc6a
https://doi.org/10.1016/j.jim.2004.04.009
https://doi.org/10.1016/j.jim.2004.04.009
Autor:
Robert W. Siegel, Yik Andy Yeung, Peter Heinzelman, James R. Coleman, Christilyn P Graff, Lee K. Opresko, H. Steven Wiley, Jennifer R. Cochran, K. Dane Wittrup, David W. Colby, Michael J. Feldhaus, Jeffrey S. Swers, Jane M. Weaver Feldhaus
Publikováno v:
Nature Biotechnology. 21:163-170
A nonimmune library of 10(9) human antibody scFv fragments has been cloned and expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by magnetic bead screening and flow-cytometric sorting. The yeast library can be amplifi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb8d834cab315446b8045484a6c1711f
https://doi.org/10.1038/nbt785
https://doi.org/10.1038/nbt785
Publikováno v:
The Journal of Immunology. 161:6280-6287
ICAM-3 is a preferred counterreceptor for the leukocyte αLβ2 integrin. It activates T cells through outside-in signaling, but polymorphonuclear leukocytes (PMN) are reported to be refractory to ICAM-3 stimulation. We found that engagement of ICAM-3
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b5f4b9b09b7139e543d6b1c176f4ed20
https://doi.org/10.4049/jimmunol.161.11.6280
https://doi.org/10.4049/jimmunol.161.11.6280
Autor:
Leonard A. Smith, Norman C. Anheier, Jay W. Grate, Marvin G. Warner, Cynthia J. Bruckner-Lea, Jianlong Lou, James D. Marks, Brian P. Dockendorff, Susan M. Varnum, Michael J. Feldhaus
Publikováno v:
Analytica chimica acta. 570(2)
Two immunoassay platforms were developed for either the sensitive or rapid detection of botulinum neurotoxin A (BoNT/A), using high-affinity recombinant monoclonal antibodies against the receptor binding domain of the heavy chain of BoNT/A. These ant
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::831ba3fddf3f91bd0d2aa16902bd789b
https://pubmed.ncbi.nlm.nih.gov/17723391
https://pubmed.ncbi.nlm.nih.gov/17723391
Autor:
Michael J. Feldhaus, Robert W. Siegel, Sean A. Gray, Keith D. Miller, Jane M. Weaver-Feldhaus
Publikováno v:
Protein expression and purification. 42(2)
Single chain (scFv) antibodies are used as affinity reagents for diagnostics, therapeutics, and proteomic analyses. The antibody discovery platform we use to identify novel antigen binders involves discovery, characterization, and production. The dis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f97fa54bb4afda913971924c75d58e1b
https://pubmed.ncbi.nlm.nih.gov/15946857
https://pubmed.ncbi.nlm.nih.gov/15946857
Autor:
Robert W. Siegel, Michael J. Feldhaus
A method to screen and isolate antigen specific clones from a library of single-chain antibodies expressed on the surface of yeast cells is presented. Two rounds of magnetic bead enrichment before flow cytometric sorting enables one to screen librari
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::28c4852374c3a90ed7562d0dafe7918e
https://doi.org/10.1385/1-59259-773-4:311
https://doi.org/10.1385/1-59259-773-4:311