Zobrazeno 1 - 10
of 101
pro vyhledávání: '"Michael J. Chamberlin"'
Publikováno v:
Biochemistry. 42:3798-3811
Abortive initiation and promoter escape are two principal biochemical reactions occurring in the latter stage of transcript initiation. We have analyzed the influences of individual DNA elements within the promoter recognition region (PRR) on these r
Publikováno v:
#N# Bacillus subtilis and Other Gram-Positive Bacteria
This chapter reviews the genetics and mapping of fla-che-mot genes, then the mechanisms of motility and chemotaxis, and finally, what is known about regulation of gene expression in the fla-che-mot system. It also concentrates on some important class
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d3e9273dc5dc56b985d00287edf7d3c7
https://doi.org/10.1128/9781555818388.ch53
https://doi.org/10.1128/9781555818388.ch53
Publikováno v:
Biochemistry. 38:218-225
Ternary complexes of RNA polymerase containing the DNA template and nascent RNA are the intermediates in transcript elongation in all cells. We have footprinted the RNA transcript with single-strand-specific ribonucleases in ternary complexes of Esch
Publikováno v:
Journal of Bacteriology. 178:24-34
We have cloned the Escherichia coli fliAZY operon, which contains the fliA gene (the alternative sigma factor sigma F) and two novel genes, fliZ and fliY. Transcriptional mapping of this operon shows two start sites, one of which is preceded by a can
Publikováno v:
Journal of Bacteriology. 176:4492-4500
The sigma D form of RNA polymerase from Bacillus subtilis has been shown previously to direct the synthesis of several transcription units bearing genes for flagellin, motility proteins, and autolysins. In this report, we describe an operon of genes
Publikováno v:
Proceedings of the National Academy of Sciences. 91:3784-3788
In the absence of DNA, Escherichia coli RNA polymerase (EC 2.7.7.6) can bind RNA to form an equimolar binary complex with the concomitant release of the sigma factor. We show now that E. coli RNA polymerase binds at a region near the 3' terminus of t
Publikováno v:
Journal of Bacteriology. 176:2427-2434
The sigma D factor of Bacillus subtilis is required for the transcription of the flagellin and motility genes as well as for wild-type chemotaxis. Southern blot and sequence analyses demonstrate that the structural gene for sigma D, sigD, is located
Publikováno v:
Nucleic Acids Research. 21:113-118
Elongation complexes of RNA polymerase II, RNA-DNA-enzyme ternary complexes, are intermediates in the synthesis of all eukaryotic mRNAs and are potential regulatory targets for factors controlling RNA chain elongation and termination. Analysis of suc
Publikováno v:
Journal of Bacteriology. 174:4197-4204
Two genes controlling motility functions in Bacillus subtilis were identified by DNA sequence analysis of a chromosomal fragment containing a strong promoter for sigma D RNA polymerase. Previous studies had shown that this sigma D-dependent promoter
Autor:
Michael J. Chamberlin, Leticia M. Marquez-Magańa, Phillip B. Carpenter, David W. Hanlon, George W. Ordal
Publikováno v:
Journal of Biological Chemistry. 267:12055-12060
A Bacillus subtilis open reading frame (ORF) encoding a predicted polypeptide of 156 amino acids was subcloned and sequenced. The polypeptide was found to be homologous to CheW of Escherichia coli, sharing 28.6% amino acid identity. The ORF was verif