Zobrazeno 1 - 10
of 34
pro vyhledávání: '"Michael J. Brisco"'
Publikováno v:
BioTechniques, Vol 49, Iss 6, Pp 893-897 (2010)
Optimal accuracy of quantitative PCR (qPCR) requires correction for integrity of the target sequence. Here we combine the mathematics of the Poisson distribution and exponential amplification to show that the frequency of lesions per base (which prev
Externí odkaz:
https://doaj.org/article/50c606eb95864dcb94d24a2cf492ae8f
Publikováno v:
Analytical Biochemistry. 409:176-182
To overcome the disadvantages of two-round nested PCR, we developed a simple and robust closed single-tube nested PCR method (antisense PCR). The method uses antisense oligonucleotides that carry a 5' tag and that can potentially hybridize to the 3'
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cdd3be186c883a8fb6ac0ccc2323034
https://doi.org/10.1016/j.ab.2010.10.030
https://doi.org/10.1016/j.ab.2010.10.030
Publikováno v:
BioTechniques. 49:893-897
Optimal accuracy of quantitative PCR (qPCR) requires correction for integrity of the target sequence. Here we combine the mathematics of the Poisson distribution and exponential amplification to show that the frequency of lesions per base (which prev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec5dbb9d0d8d6173037ea0dd473003d0
https://doi.org/10.2144/000113567
https://doi.org/10.2144/000113567
Publikováno v:
Journal of Immunological Methods. 308:1-12
We have established a method to estimate the number of clones in peripheral blood, using rearranged T cell receptor γ genes as clonal markers, selecting cells at random, and establishing the sizes of the clones to which they belong. Clone sizes were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8d62f9a08be5bf2ca061132d5e7da567
https://doi.org/10.1016/j.jim.2005.08.016
https://doi.org/10.1016/j.jim.2005.08.016
Autor:
Michael J. Brisco, L Peng, S H Neoh, Alexander A. Morley, Gaynor Dolman, Lesley Snell, Pamela J. Sykes, Elizabeth Hughes, Colin Story, I. Toogood, M. Rice, Kevin Cheney
Publikováno v:
Leukemia. 15:385-390
The level of minimal residual disease (MRD) early in treatment of acute lymphoblastic leukemia (ALL) strongly predicts the risk of marrow relapse. As a variety of methods of varying complexity have been separately used for detecting and quantifying M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91282dbdb9ee743d9c9f2a22e247cd60
https://doi.org/10.1038/sj.leu.2402044
https://doi.org/10.1038/sj.leu.2402044
Publikováno v:
Journal of Clinical Pathology. 52:766-769
A rapid method based on fluorescence resonance energy transfer (FRET) and real time polymerase chain reaction (PCR) was used to identify the haemochromatosis genotype in 112 individuals and the factor V genotype in 134 individuals. The results were c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6698e959d4cd5faef92f89a7de35411b
https://doi.org/10.1136/jcp.52.10.766
https://doi.org/10.1136/jcp.52.10.766
Autor:
Michael J. Brisco, Pamela J. Sykes, Alec Morley, Ross D. Brown, Xiao-Feng Luo, John Gibson, Doug Joshua
Publikováno v:
American Journal of Hematology. 58:273-277
Individual myeloma cells in the peripheral blood of 7 patients with multiple myeloma were detected by mRNA in situ hybridization (ISH) using biotinylated antisense oligonucleotide probes to non-germline sequences of the CDR3 region of the immunoglobu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::571ea50390fe234b51a492c41ee0c8b1
https://doi.org/10.1002/(sici)1096-8652(199808)58:4<273::aid-ajh4>3.0.co
https://doi.org/10.1002/(sici)1096-8652(199808)58:4<273::aid-ajh4>3.0.co
Publikováno v:
Analytical Biochemistry. 262:9-16
A simple method has been developed to assess strand breaks in extracted DNA. The method uses the enzyme terminal deoxynucleotidyl transferase (TDT) to incorporate labeled deoxycytidine triphosphate (dCTP) in the presence of dideoxy-CTP (ddCTP) which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d3c6484082e1672477c0d1e67a3826b8
https://doi.org/10.1006/abio.1998.2737
https://doi.org/10.1006/abio.1998.2737
Autor:
Michael J. Brisco, Pamela J. Sykes, Julianne Henry, Luen Bik To, Christopher A. Juttner, Alexander A. Morley
Publikováno v:
British Journal of Haematology. 92:614-619
It could be speculated for patients with myeloma and other lymphoproliferative disorders that peripheral blood stem cells may be preferable to bone marrow for autologous transplantation because they may be less contaminated by neoplastic cells. To te
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1c210266bd1716dcc5662199af3b5ed
https://doi.org/10.1046/j.1365-2141.1996.00381.x
https://doi.org/10.1046/j.1365-2141.1996.00381.x
Publikováno v:
Bone and Joint Institute
Heavy and light chain variable region nucleotide sequences were derived from 6 human hybridoma antibodies which bear characteristics of antibodies associated with the phospholipid antibody syndrome. All antibodies originated from non-autoimmune indiv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4a393b073ca6d06f86316d2836f7971
https://doi.org/10.3109/08916939508995299
https://doi.org/10.3109/08916939508995299