VHEE FLASH sparing effect measured at CLEAR, CERN with DNA damage of pBR322 plasmid as a biological endpoint

Autor: Hannah C. Wanstall, Pierre Korysko, Wilfred Farabolini, Roberto Corsini, Joseph J. Bateman, Vilde Rieker, Abigail Hemming, Nicholas T. Henthorn, Michael J. Merchant, Elham Santina, Amy L. Chadwick, Cameron Robertson, Alexander Malyzhenkov, Roger M. Jones

Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)

Abstract Ultra-high dose rate (UHDR) irradiation has been shown to have a sparing effect on healthy tissue, an effect known as ‘FLASH’. This effect has been studied across several radiation modalities, including photons, protons and clinical ener

Externí odkaz: https://doaj.org/article/61a9decb02b44d5288fc685095853dbb

First in vitro measurement of VHEE relative biological effectiveness (RBE) in lung and prostate cancer cells using the ARES linac at DESY

Autor: Hannah C. Wanstall, Florian Burkart, Hannes Dinter, Max Kellermeier, Willi Kuropka, Frank Mayet, Thomas Vinatier, Elham Santina, Amy L. Chadwick, Michael J. Merchant, Nicholas T. Henthorn, Michael Köpke, Blae Stacey, Sonja Jaster-Merz, Roger M. Jones

Publikováno v: Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)

Abstract Very high energy electrons (VHEE) are a potential candidate for radiotherapy applications. This includes tumours in inhomogeneous regions such as lung and prostate cancers, due to the insensitivity of VHEE to inhomogeneities. This study expl

Externí odkaz: https://doaj.org/article/bfb410ec9cca477e9bd0fe99839476d7

A computational approach to quantifying miscounting of radiation-induced double-strand break immunofluorescent foci

Autor: Samuel P. Ingram, John-William Warmenhoven, Nicholas T. Henthorn, Amy L. Chadiwck, Elham E. Santina, Stephen J. McMahon, Jan Schuemann, Norman F. Kirkby, Ranald I. Mackay, Karen J. Kirkby, Michael J. Merchant

Publikováno v: Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)

PyFoci is a tool that simulates distributions of fluorescently labeled DNA double-strand break marker protein foci and allows the estimation of miscounting under different radiation types, doses and microscopy settings.

Externí odkaz: https://doaj.org/article/12f2d24396fc42b6b08bfd133060527c

The suitability of micronuclei as markers of relative biological effect

Autor: Charlotte J Heaven, Hannah C Wanstall, Nicholas T Henthorn, John-William Warmenhoven, Samuel P Ingram, Amy L Chadwick, Elham Santina, Jamie Honeychurch, Christine K Schmidt, Karen J Kirkby, Norman F Kirkby, Neil G Burnet, Michael J Merchant

Publikováno v: Heaven, C J, Wanstall, H C, Henthorn, N T, Warmenhoven, J-W, Ingram, S P, Chadwick, A L, Santina, E, Honeychurch, J, Schmidt, C K, Kirkby, K J, Kirkby, N F, Burnet, N G & Merchant, M J 2022, ' The suitability of micronuclei as markers of relative biological effect ', Mutagenesis, vol. 37, no. 1, pp. 3-12 . https://doi.org/10.1093/mutage/geac001
Mutagenesis

Micronucleus (MN) formation is routinely used as a biodosimeter for radiation exposures and has historically been used as a measure of DNA damage in cells. Strongly correlating with dose, MN are also suggested to indicate radiation quality, different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b49bf05d1313e5ad799a37f6ed467a7d
https://doi.org/10.1093/mutage/geac001

Towards harmonizing clinical linear energy transfer (LET) reporting in proton radiotherapy: A European multi-centric study

Autor: Olga Sokol, Faiza Bourhaleb, Maria Fuglsang Jensen, Michael J. Merchant, A. M. M. Leite, Alexandru Dasu, Christian Hahn, Jakob Ödén, Chris J Rose, Claudia Pardi, E. Smith, Armin Lühr, Leszek Grzanka, Karen J. Kirkby, Anne Vestergaard, Jörg Pawelke, A. Aitkenhead, Ludovic De Marzi

Publikováno v: Acta Oncologica 61(2022)2, 206-214
Acta oncologica / Supplement 61(2), 206-214 (2022). doi:10.1080/0284186X.2021.1992007
Hahn, C, Dasu, A, Vestergaard, A, Fulsang Jensen, M, Sokol, O, Pardi, C, Bourhaleb, F, Leite, A, de Marzi, L, Smith, E, Aitkenhead, A, Rose, C, Merchant, M, Kirkby, K, Grzanka, L, Pawelke, J & Luhr, A 2021, ' Towards harmonizing clinical linear energy transfer (LET) reporting in proton radiotherapy: A European multi-centric study ', Acta Oncologica, pp. 1-9 . https://doi.org/10.1080/0284186X.2021.1992007
Acta Oncol
Hahn, C, Ödén, J, Dasu, A, Vestergaard, A, Fuglsang Jensen, M, Sokol, O, Pardi, C, Bourhaleb, F, Leite, A, de Marzi, L, Smith, E, Aitkenhead, A, Rose, C, Merchant, M, Kirkby, K, Grzanka, L, Pawelke, J & Lühr, A 2022, ' Towards harmonizing clinical linear energy transfer (LET) reporting in proton radiotherapy : a European multi-centric study ', Acta Oncologica, vol. 61, no. 2, pp. 206-214 . https://doi.org/10.1080/0284186X.2021.1992007

Background: Clinical data suggest that the relative biological effectiveness (RBE) in proton therapy (PT) varies with linear energy transfer (LET). However, LET calculations are neither standardized nor available in clinical routine. Here, the status

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f851347d18c5a036ae34028a2ad8452
https://www.hzdr.de/publications/Publ-32813-1