Zobrazeno 1 - 10
of 58
pro vyhledávání: '"Michael I. Schimerlik"'
Publikováno v:
Protein Science. 11:2113-2124
Studies with the homodimeric recombinant human macrophage colony-stimulating factor beta (rhM-CSFbeta), show for the first time that a large number (9) of disulfide linkages can be reduced after amide hydrogen/deuterium (H/D) exchange, and the protei
Autor:
Xuguang Yan, Michael I. Schimerlik, David Broderick, Max L. Deinzer, Marcia I. Dawson, Mark Leid
Publikováno v:
Journal of the American Society for Mass Spectrometry. 17:1510-1517
Several different agonists of the retinoic X receptor alpha (hRXRalpha) were examined for their effects on the amide H/D exchange kinetics of the homodimeric protein using mass spectrometry. Some agonists, LG 100268, SR11246, and DHA, bind such that
Publikováno v:
Protein Science. 13:2207-2222
A new method for analyzing three-state protein unfolding equilibria is described that overcomes the difficulties created by direct effects of denaturants on circular dichroism (CD) and fluorescence spectra of the intermediate state. The procedure beg
Publikováno v:
Biochemistry. 43:909-917
Receptors for retinoic acid act as ligand activated transcription factors. The three-dimensional structure of the retinoid X receptor (RXR) ligand binding domain has been determined, but little information is available concerning the properties of th
Autor:
Philip R. Gafken, Andrew M. Hau, Jane E. Ishmael, Michael I. Schimerlik, Gaurav Bajaj, Peter L. Hsu
Publikováno v:
Biochemical and biophysical research communications. 444(4)
N-methyl- d -aspartate (NMDA) receptors are calcium-permeable ion channels assembled from four subunits that each have a common membrane topology. The intracellular carboxyl terminal domain (CTD) of each subunit varies in length, is least conserved b
Publikováno v:
Biochemistry. 41:205-214
The pH dependence of basal and calmodulin- (CaM-) stimulated neuronal nitric oxide synthase (nNOS) reduction of 2,6-dichloroindophenol (DCIP) and cytochrome c 3 + was investigated. The wave-shaped log V versus pH profile revealed that optimal DCIP re
Publikováno v:
Biochemistry. 41:196-204
The neuronal nitric oxide synthase (nNOS) basal and calmodulin- (CaM-) stimulated reduction of 2,6-dichloroindophenol (DCIP) and cytochrome c(3+) follow ping-pong mechanisms [Wolthers and Schimerlik (2001) Biochemistry 40, 4722-4737]. Primary deuteri
Autor:
Ljiljana Paša-Tolić, Richard D. Smith, Claudia S. Maier, Max L. Deinzer, Xuguang Yan, Michael I. Schimerlik, Mark E. Harder
Publikováno v:
Journal of the American Society for Mass Spectrometry. 11:237-243
The potential of electrospray ionization (ESI) Fourier transform ion cyclotron mass spectrometry (FTICR-MS) to assist in the structural characterization of monomeric and dimeric derivatives of the macrophage colony stimulating factor beta (rhM-CSF be
Autor:
Marita C. Barth, George S. Bailey, William M. Baird, Jerry D. Hendricks, Ulrich Harttig, Ashok P. Reddy, Michael I. Schimerlik
Publikováno v:
Carcinogenesis. 20:1919-1926
Cancer chemoprevention by dietary chlorophyllin (CHL) was investigated in a rainbow trout multi-organ tumor model. In study 1, duplicate groups of 130 juvenile trout were treated for 2 weeks with control diet, 500 p.p.m. dibenzo[a,l]pyrene (DB[a,l]P)
Publikováno v:
Toxicology and Applied Pharmacology. 158:132-140
Chlorophyllin (CHL) is a potent blocking agent against aflatoxin B 1 DNA adduction and tumorigenesis in the trout model, but mechanisms responsible for this chemoprotection in vivo are not well established. This study employed aflatoxin B 2 (AFB 2 ),