Zobrazeno 1 - 10
of 107
pro vyhledávání: '"Michael H Brodsky"'
Publikováno v:
PLoS ONE, Vol 9, Iss 9, p e108424 (2014)
CRISPR-Cas systems are a diverse family of RNA-protein complexes in bacteria that target foreign DNA sequences for cleavage. Derivatives of these complexes have been engineered to cleave specific target sequences depending on the sequence of a CRISPR
Externí odkaz:
https://doaj.org/article/29df13dda54243ecb28af0f727b20237
Autor:
Qiong Cheng, Majid Kazemian, Hannah Pham, Charles Blatti, Susan E Celniker, Scot A Wolfe, Michael H Brodsky, Saurabh Sinha
Publikováno v:
PLoS Genetics, Vol 9, Iss 8, p e1003571 (2013)
ChIP-based genome-wide assays of transcription factor (TF) occupancy have emerged as a powerful, high-throughput method to understand transcriptional regulation, especially on a global scale. This has led to great interest in the underlying biochemic
Externí odkaz:
https://doaj.org/article/85068967f9a24538805497de4fa60eba
Publikováno v:
PLoS Genetics, Vol 7, Iss 10, p e1002315 (2011)
DNA repair mechanisms in mitotically proliferating cells avoid generating crossovers, which can contribute to genome instability. Most models for the production of crossovers involve an intermediate with one or more four-stranded Holliday junctions (
Externí odkaz:
https://doaj.org/article/49c1c69e401e4f4fa86188b7c2c0eac2
Autor:
Majid Kazemian, Charles Blatti, Adam Richards, Michael McCutchan, Noriko Wakabayashi-Ito, Ann S Hammonds, Susan E Celniker, Sudhir Kumar, Scot A Wolfe, Michael H Brodsky, Saurabh Sinha
Publikováno v:
PLoS Biology, Vol 8, Iss 8, p e1000456 (2010)
Cis-regulatory modules that drive precise spatial-temporal patterns of gene expression are central to the process of metazoan development. We describe a new computational strategy to annotate genomic sequences based on their "pattern generating poten
Externí odkaz:
https://doaj.org/article/8623d3269ad24e4f923079e9b1f726d1
Autor:
Sarah R Oikemus, Joana Queiroz-Machado, KuanJu Lai, Nadine McGinnis, Claudio Sunkel, Michael H Brodsky
Publikováno v:
PLoS Genetics, Vol 2, Iss 5, p e71 (2006)
Analysis of terminal deletion chromosomes indicates that a sequence-independent mechanism regulates protection of Drosophila telomeres. Mutations in Drosophila DNA damage response genes such as atm/tefu, mre11, or rad50 disrupt telomere protection an
Externí odkaz:
https://doaj.org/article/d66089bdd46147beb2bc25b058433c18
Autor:
Katharina E. Meijboom, Abbas Abdallah, Nicholas P. Fordham, Hiroko Nagase, Tomás Rodriguez, Carolyn Kraus, Tania F. Gendron, Gopinath Krishnan, Rustam Esanov, Nadja S. Andrade, Matthew J. Rybin, Melina Ramic, Zachary D. Stephens, Alireza Edraki, Meghan T. Blackwood, Aydan Kahriman, Nils Henninger, Jean-Pierre A. Kocher, Michael Benatar, Michael H. Brodsky, Leonard Petrucelli, Fen-Biao Gao, Erik J. Sontheimer, Robert H. Brown, Zane Zeier, Christian Mueller
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
A hexanucleotide repeat expansion in C9ORF72 is the most common genetic cause of ALS and FTD. Here, the authors demonstrate CRISPR/Cas9 excision of the expansion results in a rescue of disease mechanisms in vivo and in vitro.
Externí odkaz:
https://doaj.org/article/3c12d94acb99423da250fefe20d4cde2
Autor:
Janice A. Dominov, Laura A. Madigan, Joshua P. Whitt, Katerina L. Rademacher, Kristin M. Webster, Hesheng Zhang, Haruhiko Banno, Siqi Tang, Yifan Zhang, Nicholas Wightman, Emma M. Shychuck, John Page, Alexandra Weiss, Karen Kelly, Alper Kucukural, Michael H. Brodsky, Alexander Jaworski, Justin R. Fallon, Diane Lipscombe, Robert H. Brown
Publikováno v:
bioRxiv
Amyotrophic lateral sclerosis (ALS) is a severe neurodegenerative disorder affecting brain and spinal cord motor neurons. Mutations in the copper/zinc superoxide dismutase gene (SOD1) are associated with ∼20% of inherited and 1-2% of sporadic ALS c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c0de160fc2e91574d01f7d30c4af0729
https://doi.org/10.1101/2023.05.05.539444
https://doi.org/10.1101/2023.05.05.539444
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 6, Iss 10, Pp 3197-3206 (2016)
Adoption of a streamlined version of the bacterial clustered regular interspersed short palindromic repeat (CRISPR)/Cas9 defense system has accelerated targeted genome engineering. The Streptococcus pyogenes Cas9 protein, directed by a simplified, CR
Externí odkaz:
https://doaj.org/article/ca144083ff2a422cb941669ca1370127
Autor:
Ciearra B. Smith, Vincent van der Vinne, Eleanor McCartney, Adam C. Stowie, Tanya L. Leise, Blanca Martin-Burgos, Penny C. Molyneux, Lauren A. Garbutt, Michael H. Brodsky, Alec J. Davidson, Mary E. Harrington, Robert Dallmann, David R. Weaver
Publikováno v:
Journal of Biological Rhythms. 37:53-77
Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report developmen
Autor:
Ellen Sapp, Rachael Miller, Kathryn Chase, Sarah R. Oikemus, Eric Mick, Lihua Julie Zhu, Michael H. Brodsky, Miguel Sena-Esteves, Edward Hudgens, Akanksh Chaudhary, Lori A. Kennington, Scot A. Wolfe, Neil Aronin, Marian DiFiglia, Edith L. Pfister
Publikováno v:
Hum Gene Ther
Huntington's disease (HD) is a devastating, autosomal dominant neurodegenerative disease caused by a trinucleotide repeat expansion in the huntingtin (HTT) gene. Inactivation of the mutant allele by clustered regularly interspaced short palindromic r