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Autor:
Martin Wegner, Valentina Diehl, Verena Bittl, Rahel de Bruyn, Svenja Wiechmann, Yves Matthess, Marie Hebel, Michael GB Hayes, Simone Schaubeck, Christopher Benner, Sven Heinz, Anja Bremm, Ivan Dikic, Andreas Ernst, Manuel Kaulich
Publikováno v:
eLife, Vol 8 (2019)
Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore, increasing gRNA sequence diversity negatively affects gRNA distribution, leading to libraries
Externí odkaz:
https://doaj.org/article/393c8588725544a5ab186bfe7246d237