Using bacteria to determine protein kinase specificity and predict target substrates.

Autor: Michael F Chou, Sladjana Prisic, Joshua M Lubner, George M Church, Robert N Husson, Daniel Schwartz

Publikováno v: PLoS ONE, Vol 7, Iss 12, p e52747 (2012)

The identification of protein kinase targets remains a significant bottleneck for our understanding of signal transduction in normal and diseased cellular states. Kinases recognize their substrates in part through sequence motifs on substrate protein

Externí odkaz: https://doaj.org/article/74986d65be9f43b0a21e7ec887eb6ebc

Cushing's syndrome mutant PKA L 205R exhibits altered substrate specificity

Autor: Kimberly L. Dodge-Kafka, Joshua M. Lubner, George M. Church, Cathrine R. Carlson, Michael F. Chou, Daniel Schwartz

Publikováno v: FEBS Letters. 591:459-467

The PKAL205R hotspot mutation has been implicated in Cushing's syndrome through hyperactive gain-of-function PKA signaling; however, its influence on substrate specificity has not been investigated. Here, we employ the Proteomic Peptide Library (ProP

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::15d98f9154ed0b4817265d9ce3889773
https://doi.org/10.1002/1873-3468.12562

Cushing’s Syndrome mutant PKAL205R exhibits altered substrate specificity

Autor: Kimberly L. Dodge-Kafka, Daniel Schwartz, Joshua M. Lubner, George M. Church, Cathrine R. Carlson, Michael F. Chou

The PKAL205R hotspot mutation has been implicated in Cushing’s Syndrome through hyperactive gain-of-function PKA signaling, however its influence on substrate specificity has not been investigated. Here, we employ the Proteomic Peptide Library (Pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c1b5afd99c0641f9a5873e3d60d6606
https://doi.org/10.1101/091231

A public resource facilitating clinical use of genomes

Autor: John Aach, Euan A. Ashley, Je-Hyuk Lee, Hugh Y. Rienhoff, Matthew T. Wheeler, Jong Bhak, Peter J. Hulick, Joyce L. Yang, Carlos Cano, Radoje Drmanac, Jeong-Sun Seo, Jong Il Kim, Athurva Gore, Anugraha M. Raman, Zhe Li, Xiaodi Wu, Shawn M. Douglas, Michael F. Chou, Daniel B. Vorhaus, Jin Billy Li, Alberto Labarga, Michael F. Murray, Kimberly Robasky, Seong-Jin Kim, Byung Chul Kim, Leonid Peshkin, Luhan Yang, George M. Church, Misha Angrist, Joseph V. Thakuria, Preston W. Estep, Jeantine E. Lunshof, Christine E. Seidman, Madeleine Ball, Tom Clegg, Alexander Wait Zaranek, Abraham M. Rosenbaum, Brock A. Peters, Wendy K. Chung, Ward Vandewege, Heidi L. Rehm, Geoffrey B. Nilsen, Matthew J. Callow, Kun Zhang, Jason Bobe

Publikováno v: Ball, M P, Thakuria, J V, Zaranek, A W, Clegg, T, Rosenbaum, A M, Wu, X, Angrist, M, Bhak, J, Bobe, J, Callow, M J, Cano, C, Chou, M F, Chung, W K, Douglas, S M, Estep, P W, Gore, A, Hulick, P, Labarga, A, Lee, J-H, Lunshof, J E, Kim, B C, Kim, J L, Li, Z, Murray, M F, Nilsen, G B, Peters, B A, Raman, A M, Rienhoff, H Y, Robasky, K, Wheeler, M T, Vandewege, W, Vorhaus, D B, Yang, Y L, Yang, L, Aach, J, Ashley, E A, Drmanac, R, Kim, S-J, Li, J B, Peshkin, L, Seidman, S E, Seo, J-S, Zhang, K, Rehm, H L & Church, G M 2012, ' A public resource facilitating clinical use of genomes. ', Proceedings of the National Academy of Sciences of the United States of America, vol. 109, no. 30, pp. 11920-11927 . https://doi.org/10.1073/pnas.1201904109
Proceedings of the National Academy of Sciences of the United States of America, 109(30), 11920-11927. National Acad Sciences
Proceedings of the National Academy of Sciences of the United States of America, 109(30), 11920-11927. National Academy of Sciences
Proceedings of the National Academy of Sciences of the United States of America, vol 109, iss 30

Rapid advances in DNA sequencing promise to enable new diagnostics and individualized therapies. Achieving personalized medicine, however, will require extensive research on highly reidentifiable, integrated datasets of genomic and health information

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47e2236be047d04a80fe8cbb08175d3e
https://research.vu.nl/en/publications/6e1a1cef-c7ef-4868-b615-c658b7a3ea72

Clinical assessment incorporating a personal genome

Autor: Matthew T. Wheeler, Stephen R. Quake, Euan A. Ashley, Laura M. Hodges, Joseph V. Thakuria, Joshua W. Knowles, Kelly E. Ormond, Dmitry Pushkarev, Joan M. Hebert, Joel T. Dudley, Mark Woon, Frederick E. Dewey, Katrin Sangkuhl, Alexander A. Morgan, Alexander Wait Zaranek, Dorit S. Berlin, Abraham M. Rosenbaum, Louanne Hudgins, Michael F. Chou, Rong Chen, Atul J. Butte, George M. Church, Aleksandra Pavlovic, Teri E. Klein, Caroline F. Thorn, Henry T. Greely, Li Gong, Ryan Whaley, Russ B. Altman, Norma F. Neff, Hersh Sagreiya

Publikováno v: The Lancet. 375:1525-1535

Summary Background The cost of genomic information has fallen steeply, but the clinical translation of genetic risk estimates remains unclear. We aimed to undertake an integrated analysis of a complete human genome in a clinical context. Methods We a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8d4f54bf2e1f647abdda37f15e523a5
https://doi.org/10.1016/s0140-6736(10)60452-7