Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Michael E. Kovach"'
Publikováno v:
Molecular Microbiology. 35:577-588
The gene encoding a Lon protease homologue has been cloned from Brucella abortus. The putative Brucella abortus Lon shares > 60% amino acid identity with its Escherichia coli counterpart and the recombinant form of this protein restores the capacity
Autor:
Michael E. Kovach, Kenneth M. Peterson, Gregory T. Robertson, Melissa A Christensen, Rebecca L. Chirhart-Gilleland, R. Martin Roop, Philip H. Elzer
Publikováno v:
Microbial Pathogenesis. 22:241-246
A DNA fragment encoding an approximately 18 kDa protein from Brucella abortus strain 2308 was cloned and expressed in Escherichia coli. This recombinant protein, designated BA18K, reacted in Western blot analysis with sera obtained from experimentall
Publikováno v:
Microbiology. 142:2165-2174
A large cluster of virulence genes encoding proteins involved in Vibrio cholerae accessory colonization factor (ACF) expression and toxin-coregulated pilus (TCP) biogenesis is flanked by sequences that resemble bacteriophage attachment (att) half-sit
Publikováno v:
Gene. 148:91-95
The nucleotide (nt) sequence has been determined for a Vibrio cholerae ToxR-activated gene designated tagE that is located within a cluster of genes required for efficient intestinal colonization. The tagE gene encompasses 909 nt and is predicted to
Publikováno v:
Infection and Immunity. 62:4135-4139
In order to evaluate the biological function of the Brucella abortus high-temperature-requirement A (HtrA) stress response protein homolog, the majority of the htrA gene was deleted from the chromosome of B. abortus 2308 via gene replacement. In cont
Publikováno v:
Infection and Immunity. 62:3289-3298
Vibrio cholerae accessory colonization factor genes (acfA, B, C, and D) are required for efficient intestinal colonization. Expression of acf genes is under the control of a regulatory cascade that also directs the synthesis of cholera toxin and prot
Autor:
Jason M. Gee, R. Martin Roop, Wai-Leung Ng, Michael E. Kovach, Gregory T. Robertson, Michelle Wright Valderas, John M. Richardson, Vanessa K. Grippe, Malcolm E. Winkler
Publikováno v:
Infection and immunity. 73(5)
Two-dimensional gel electrophoretic analysis of cell lysates fromBrucella abortus2308 and the isogenichfqmutant Hfq3 revealed that the RNA binding protein Hfq (also known as host factor I or HF-I) is required for the optimal stationary phase producti
Autor:
Philip H. Elzer, Joel V. Walker, Sue D. Hagius, Jason M. Gee, Michael E. Kovach, Vanessa K. Grippe, R. Martin Roop
Publikováno v:
Veterinary microbiology. 102(1-2)
An isogenic katE mutant derived from virulent Brucella melitensis 16M displays hypersensitivity to hydrogen peroxide in disk sensitivity assays but retains the capacity to colonize pregnant goats and induce abortion. These experimental findings indic
Autor:
Rebecca L. Chirhart-Gilleland, Philip H. Elzer, Michael E. Kovach, R. Martin Roop, Stephen R. Jennings
Publikováno v:
Infection and immunity. 66(8)
A low-molecular-weight recombinant Brucella abortus protein reactive with antibodies from a variety of naturally and experimentally infected hosts and T lymphocytes from experimentally infected mice was identified and given the designation BA14K. The
Autor:
Philip H. Elzer, D. Steven Hill, Michael E. Kovach, Kenneth M. Peterson, R. Martin Roop, Gregory T. Robertson, Michael A. Farris
Publikováno v:
Gene. 166(1)
Four new antibiotic-resistant derivatives of the broad-host-range (bhr) cloning vector pBBR1MCS have been constructed. These new plasmids have several advantages over many of the currently available bhr vectors in that: (i) they are relatively small