Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Michael Derek Edge"'
Autor:
I. Hodgson, Michael Derek Edge, A.F. Markham, David Holland, Michael Joseph Mclean, C.R. Newton, L.E. Heptinstall
Publikováno v:
Nucleic acids research. 21(5)
We have prepared several novel phosphoramidites and have synthesised oligonucleotides incorporating them internally. The presence of these residues in an oligonucleotide template presents an impossible barrier to primed synthesis by Taq DNA polymeras
Publikováno v:
Nucleic acids research. 18(12)
Using the endogenous histone H4 mRNA of Xenopus oocytes as a target, we have previously shown that 20mer oligos complementary to different parts of this sequence vary in their effectiveness at causing mRNA cleavage in vivo, and that some of the RNA c
Autor:
Clive R. Newton, T.C. Atkinson, G.R. Heathcliffe, A.R. Greene, Michael J. Gait, R.C. Sheppard, M. Singh, A.F. Markham, Michael Derek Edge
Publikováno v:
Nucleic Acids Research. 8:1081-1096
A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold be
Autor:
April R. Greene, A.F. Markham, Michael Derek Edge, Wolfgang Walter Schuch, Peter A. Meacock, Clive R. Newton, Gillian R. Heathcliffe, Thomas C. Atkinson, Denis B. Scanlon
Publikováno v:
Nature. 292:756-762
A 514-base pair fragment of double-stranded DNA coding for human interferon-alpha 1 (166 amino acid residues), and containing initiation and termination signals plus appropriate restriction enzyme sites for plasmid insertion, has been totally synthes
Publikováno v:
Tetrahedron Letters. 30:3089-3092
Protected D(+)-biotin methylesters 3 were reduced to the corresponding alcohols 4 and phosphitylated with chloromethoxymorpholinophosphine. The resulting phosphoramidites 5 were used on a commercial DNA synthesiser to biotinylate synthetic DNA.
Autor:
Clive R. Newton, Gillian R. Heathcliffe, Michael Derek Edge, Thomas C. Atkinson, David Holland, April R. Greene, Alexander F. Markham
Publikováno v:
Analytical Biochemistry. 129:22-30
The superiority of buffer systems containing formamide for the ion-exchange high-performance liquid chromatographic separation of oligodeoxyribonucleotide mixtures generated in solid-phase syntheses is illustrated. The resolutions achieved are compar
Autor:
A.R. Greene, G.R. Heathcliffe, Michael Derek Edge, A.F. Markham, Denis B. Scanlon, T.C. Atkinson, Clive R. Newton
Phosphotriester solid phase methodology on a polyamide support [(1980) Nucleic Acids Research, 8, 1081-1096] has been extended for the rapid synthesis of the tetradecanucleotide, d(AGTTGTTTGTAGTT), the octadecanucleotide, d(GTGGGTTTGGGGCAGGTC), and t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c4328dca358c28111ee44febf44a597
https://europepmc.org/articles/PMC324294/
https://europepmc.org/articles/PMC324294/
Autor:
Wolfgang Walter Schuch, N.N. Petter, N.J. Faulkner, A.R. Greene, J. Hennam, A.F. Markham, T.C. Atkinson, Michael Derek Edge, G.R. Heathcliffe, Clive R. Newton, V. E. Moore, P. Trueman, R. Camble
Publikováno v:
Nucleic acids research. 11(18)
A 511-base pair DNA fragment encoding human interferon-alpha 2 has been chemically synthesised and expressed from a lac UV5 and a synthetic trp promoter in Escherichia coli. The synthesis involved preparation of 68 oligodeoxyribonucleotides and their
Autor:
Roger Camble, Michael Derek Edge
Publikováno v:
Biotechnologygenetic engineering reviews. 2
Description des differentes classes d'interferon, methodes de clonage des genes et de synthese chez les microorganismes; techniques de production d'analogues: structure et proprietes therapeutiques eventuelles