Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Michael D. Diem"'
Autor:
Lauren E. Boucher, Elisabeth Geyer Prinslow, Michael Feldkamp, Fang Yi, Rupesh Nanjunda, Sheng-Jiun Wu, Tun Liu, Eilyn R. Lacy, Steven Jacobs, Natalia Kozlyuk, Brian Del Rosario, Bingyuan Wu, Patricia Aquino, Robert C. Davidson, Samantha Heyne, Nicholas Mazzanti, James Testa, Michael D. Diem, Elsa Gorre, Andrew Mahan, Hirsh Nanda, Harsha P. Gunawardena, Alexis Gervais, Anthony A. Armstrong, Alexey Teplyakov, Chichi Huang, Adam Zwolak, Partha Chowdhury, Wan Cheung Cheung, Jinquan Luo
Publikováno v:
mAbs, Vol 15, Iss 1 (2023)
ABSTRACTSingle-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells. However, scFv domains exhibit lo
Externí odkaz:
https://doaj.org/article/07a49e5767d741f0bc24b50e3af689e1
Autor:
Christine Springer, Adam Zwolak, Haiyan Jiang, Thomas C. Nesspor, Sanjaya Singh, Gordon Powers, Nicholas Mazzanti, Justin Sprenkle, Sherry L. La Porte, Kyle Kinealy, Michael D. Diem, Kevin Boye, Rajkumar Ganesan, Hunter Hoffman
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Scientific Reports
Scientific Reports
Generation of bispecific antibodies (BsAbs) having two unique Fab domains requires heterodimerization of the two heavy chains and pairing of each heavy chain with its cognate light chain. An alternative bispecific scaffold (Bipod) comprising an scFv
Autor:
Lili Wan, Michael Soo, Michael D. Diem, Congli Wang, Ihab Younis, Gideon Dreyfuss, Michael G. Berg
Publikováno v:
Molecular and Cellular Biology. 32:1271-1283
Despite intensive research, there are very few reagents with which to modulate and dissect the mRNA splicing pathway. Here, we describe a novel approach to identify such tools, based on detection of the exon junction complex (EJC), a unique molecular
Autor:
Steven Jacobs, Elise Kuhar, Karyn O'neil, Fang Yi, Linus Hyun, Randi Hippensteel, Edward J. Swift, Michael D. Diem, Cassandra Lowenstein
Publikováno v:
Protein engineering, designselection : PEDS. 27(10)
Alternative scaffold molecules represent a class of proteins important to the study of protein design and mechanisms of protein-protein interactions, as well as for the development of therapeutic proteins. Here, we describe the generation of a librar
Autor:
Thomas J. Malia, Jinquan Luo, Gary L. Gilliland, Galina Obmolova, Karyn O'neil, Steven Jacobs, Alexey Teplyakov, Michael D. Diem
Publikováno v:
Protein engineering, designselection : PEDS. 25(3)
The use of consensus design to produce stable proteins has been applied to numerous structures and classes of proteins. Here, we describe the engineering of novel FN3 domains from two different proteins, namely human fibronectin and human tenascin-C,
Autor:
Michael D. Diem, Chihiro Hatai, Mutsuhito Ohno, Masatoshi Hagiwara, Izumi Dobashi, Naoyuki Kataoka, Gideon Dreyfuss, Mayumi Yoshida
Publikováno v:
Scientific Reports
Pre-mRNA splicing deposits multi-protein complexes, termed exon junction complexes (EJCs), on mRNAs near exon-exon junctions. The core of EJC consists of four proteins, eIF4AIII, MLN51, Y14 and Magoh. Y14 is a nuclear protein that can shuttle between
Publikováno v:
Nature structuralmolecular biology. 14(12)
Messenger RNAs produced by splicing are translated more efficiently than those produced from similar intronless precursor mRNAs (pre-mRNAs). The exon-junction complex (EJC) probably mediates this enhancement; however, the specific link between the EJ
Autor:
Gideon Dreyfuss, Michael D. Diem, Josée Dostie, Matthias Mann, Juri Rappsilber, Wenqin Feng, Chia C. Chan
Publikováno v:
Chan, C C, Dostie, J, Diem, M D, Feng, W, Mann, M, Rappsilber, J & Dreyfuss, G 2004, ' eIF4A3 is a novel component of the exon junction complex. ', RNA: A publication of the RNA Society, vol. 10, pp. 200-209 .
The exon junction complex (EJC) is a protein complex that assembles near exon–exon junctions of mRNAs as a result of splicing. EJC proteins play important roles in postsplicing events including mRNA export, cytoplasmic localization, and nonsense-me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16e3a41ec5cb1beafdcf29c1178e5f8f
https://europepmc.org/articles/PMC1370532/
https://europepmc.org/articles/PMC1370532/
Publikováno v:
Current biology : CB. 13(11)
Background: Splicing of pre-mRNA in eukaryotes imprints the resulting mRNA with a specific multiprotein complex, the exon-exon junction complex (EJC), at the sites of intron removal. The proteins of the EJC, Y14, Magoh, Aly/REF, RNPS1, Srm160, and Up
Publikováno v:
The EMBO journal. 20(22)
The RNA-binding protein Y14 binds preferentially to mRNAs produced by splicing and is a component of a multiprotein complex that assembles approximately 20 nucleotides upstream of exon-exon junctions. This complex probably has important functions in