Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Michael C. Naill"'
Autor:
Susan C. Roberts, Michael C. Naill
Publikováno v:
Biotechnology Progress. 21:978-983
An immunofluorescence procedure was developed for paclitaxel quantification at the single cell level via flow cytometry in Taxus cuspidata suspension cultures. Intracellular staining was validated via fluorescence microscopy. Paclitaxel content of is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0315282e0a3939a0c1f0e26a43665dd
https://doi.org/10.1021/bp049544l
https://doi.org/10.1021/bp049544l
Autor:
Michael C. Naill, Susan C. Roberts
Publikováno v:
Biotechnology and Bioengineering. 90:491-500
Single cell growth and division was measured via flow cytometry in order to characterize the metabolic variability of Taxus cuspidata suspension cultures, which produce the valuable secondary metabolite Taxol. Good agreement was observed between the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfcf94306ba1adb01a4ac520fc21522f
https://doi.org/10.1002/bit.20446
https://doi.org/10.1002/bit.20446
Autor:
Michael C. Naill, Susan C. Roberts
Publikováno v:
Biotechnology and Bioengineering. 86:817-826
A method for the isolation of single plant cells from Taxus suspension cultures has been developed for the analysis of single cells via rapid throughput techniques such as flow cytometry. Several cell wall specific enzymes, such as pectinase, pectoly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::615968894cae66502880a9c529a9825d
https://doi.org/10.1002/bit.20083
https://doi.org/10.1002/bit.20083
Publikováno v:
Plant Cell Reports. 21:1217-1220
Paclitaxel storage in Taxus suspension cell cultures was studied through the simple use of cell wall digesting enzymes. The application of cellulase (1%) and pectolyase (0.1%) to Taxus canadensis suspension cultures induced a significant increase in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::341248e1bd25e341f7fec30ad972da8b
https://doi.org/10.1007/s00299-003-0575-z
https://doi.org/10.1007/s00299-003-0575-z
Publikováno v:
Biochemical engineering journal. 63
The transport of paclitaxel in Taxus canadensis suspension cultures was studied with a fluorescence analogue of paclitaxel (Flutax-2(®)) in combination with flow cytometry detection. Experiments were carried out using both isolated protoplasts and a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff506a292391052549e84d30d938cea5
https://pubmed.ncbi.nlm.nih.gov/23180977
https://pubmed.ncbi.nlm.nih.gov/23180977
Autor:
Susan C. Roberts, Michael C. Naill
Publikováno v:
Biotechnology letters. 27(21)
Single cells isolated from aggregated Taxus cuspidata cultures via enzymatic digestion were grown in suspension culture. High seeding density (4 x 10(5 )cells/ml) and the addition of cell-free conditioned medium were essential for growth. Doubling th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7c4b68ce135c9aa321dfbd21f122a46
https://pubmed.ncbi.nlm.nih.gov/16247682
https://pubmed.ncbi.nlm.nih.gov/16247682
Autor:
Michael C. Naill, Susan C. Roberts
Publikováno v:
Plant cell reports. 23(8)
Plant suspension cultures are highly aggregated, preventing the direct application of flow cytometry for the study of population dynamics. The utility of single cells to accurately represent aggregated suspension cultures was tested through the analy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::baf6fdd6b04aa80fa1e8d18b709e2611
https://pubmed.ncbi.nlm.nih.gov/15449019
https://pubmed.ncbi.nlm.nih.gov/15449019
Autor:
Michael C. Naill, Susan C. Roberts
Publikováno v:
Plant Cell Reports; Jan2005, Vol. 23 Issue 8, p528-533, 28p