Zobrazeno 1 - 10
of 289
pro vyhledávání: '"Michael C Jewett"'
Autor:
Jose L Alejo, Dylan Girodat, Michael J Hammerling, Jessica A Willi, Michael C Jewett, Aaron E Engelhart, Katarzyna P Adamala
Publikováno v:
PLoS Computational Biology, Vol 20, Iss 8, p e1012319 (2024)
Translocation in protein synthesis entails the efficient and accurate movement of the mRNA-[tRNA]2 substrate through the ribosome after peptide bond formation. An essential conformational change during this process is the swiveling of the small subun
Externí odkaz:
https://doaj.org/article/50452fd9ffea4d23909913eeb26cc25b
Autor:
Nolan W Kennedy, Jasmine M Hershewe, Taylor M Nichols, Eric W Roth, Charlene D Wilke, Carolyn E Mills, Michael C Jewett, Danielle Tullman-Ercek
Publikováno v:
PLoS ONE, Vol 15, Iss 3, p e0226395 (2020)
Bacterial microcompartments (MCPs) are protein-based organelles that encapsulate metabolic pathways. Metabolic engineers have recently sought to repurpose MCPs to encapsulate heterologous pathways to increase flux through pathways of interest. As MCP
Externí odkaz:
https://doaj.org/article/c68887ec6b5d42c7a55e027fd3ff4c13
Publikováno v:
Molecular Systems Biology, Vol 9, Iss 1, Pp 1-8 (2013)
Abstract Purely in vitro ribosome synthesis could provide a critical step towards unraveling the systems biology of ribosome biogenesis, constructing minimal cells from defined components, and engineering ribosomes with new functions. Here, as an ini
Externí odkaz:
https://doaj.org/article/5b75a912f863484bb66f34f557aceaa1
Publikováno v:
PLoS Computational Biology, Vol 12, Iss 11, p e1005184 (2016)
The existence of over- and under-represented sequence motifs in genomes provides evidence of selective evolutionary pressures on biological mechanisms such as transcription, translation, ligand-substrate binding, and host immunity. In order to accura
Externí odkaz:
https://doaj.org/article/81208a0efe0c4b3b98a388c687522b1d
Autor:
Renata Usaite, Michael C Jewett, Ana Paula Oliveira, John R Yates, Lisbeth Olsson, Jens Nielsen
Publikováno v:
Molecular Systems Biology, Vol 5, Iss 1, Pp 1-12 (2009)
Abstract Highly conserved among eukaryotic cells, the AMP‐activated kinase (AMPK) is a central regulator of carbon metabolism. To map the complete network of interactions around AMPK in yeast (Snf1) and to evaluate the role of its regulatory subuni
Externí odkaz:
https://doaj.org/article/083ad53bafc6419babe064a9dbdd6609
Publikováno v:
Molecular Systems Biology, Vol 4, Iss 1, Pp 1-10 (2008)
Abstract Cell‐free systems offer a unique platform for expanding the capabilities of natural biological systems for useful purposes, i.e. synthetic biology. They reduce complexity, remove structural barriers, and do not require the maintenance of c
Externí odkaz:
https://doaj.org/article/e8c405df0a1b48a88d562c599ce54146
Autor:
Joongoo Lee, Jaime N. Coronado, Namjin Cho, Jongdoo Lim, Brandon M. Hosford, Sangwon Seo, Do Soon Kim, Camila Kofman, Jeffrey S. Moore, Andrew D. Ellington, Eric V. Anslyn, Michael C. Jewett
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-9 (2022)
Ribosomes have evolved to polymerize L-α-amino acids into proteins comprising a peptide backbone. Here, a pyridazinone backbone is formed using ribosomes in vitro, producing a variety of sequence-defined alternating block-copolymers.
Externí odkaz:
https://doaj.org/article/af5f194faf92459b90bf08358de0c5a6
A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
Autor:
Thapakorn Jaroentomeechai, Yong Hyun Kwon, Yiwen Liu, Olivia Young, Ruchika Bhawal, Joshua D. Wilson, Mingji Li, Digantkumar G. Chapla, Kelley W. Moremen, Michael C. Jewett, Dario Mizrachi, Matthew P. DeLisa
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
Access to glycoenzymes for basic and applied research is limited by difficulties with their recombinant expression. Here, the authors describe a universal strategy for converting membrane-bound glycosyltransferases into water-soluble biocatalysts, wh
Externí odkaz:
https://doaj.org/article/cc7e68d73dbc4e25965f65c3d9808c86
Autor:
Yasmine S. Zubi, Kosuke Seki, Ying Li, Andrew C. Hunt, Bingqing Liu, Benoît Roux, Michael C. Jewett, Jared C. Lewis
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Dynamic control over protein function is a central challenge in synthetic biology. Here the authors present an integrated computational and experimental workflow for engineering reversible protein switches; metal-chelating unnatural amino acids genet
Externí odkaz:
https://doaj.org/article/5b92cf522672475cbe8486943e403466
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
Cell-free systems enable the design of biosynthetic pathways for sustainable chemical synthesis. Here the authors create an integrated framework for accelerating design using extracts from genetically rewired strains.
Externí odkaz:
https://doaj.org/article/73f60a6b74f34dd9b16350ac7a8f3a1f