Výsledky vyhledávání - "Michael A. Sydor"

Akademický článek

Identification of Novel Host Interactors of Effectors Secreted by Salmonella and Citrobacter

Autor: Ryan L. Sontag, Ernesto S. Nakayasu, Roslyn N. Brown, George S. Niemann, Michael A. Sydor, Octavio Sanchez, Charles Ansong, Shao-Yeh Lu, Hyungwon Choi, Dylan Valleau, Karl K. Weitz, Alexei Savchenko, Eric D. Cambronne, Joshua N. Adkins

Publikováno v: mSystems, Vol 1, Iss 4 (2016)

ABSTRACT Many pathogenic bacteria of the family Enterobacteriaceae use type III secretion systems to inject virulence proteins, termed “effectors,” into the host cell cytosol. Although host-cellular activities of several effectors have been demon

Externí odkaz: https://doaj.org/article/f78a2ab44ff5495684196c96fa5db218

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False-negative rate, limit of detection and recovery efficiency performance of a validated macrofoam-swab sampling method for low surface concentrations ofBacillus anthracisSterne andBacillus atrophaeusspores

Autor: B.L. Deatherage Kaiser, Brett G. Amidan, Christopher A. Barrett, Michael A. Sydor, Gregory F. Piepel, Janine R. Hutchison

Publikováno v: Journal of Applied Microbiology. 121:149-162

Aims We sought to evaluate the effects of Bacillus species, low surface concentrations, and surface material on recovery efficiency (RE), false-negative rate (FNR) and limit of detection for recovering Bacillus spores using a validated macrofoam-swab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1feec5c81b5f17d9822af440a3b9dee
https://doi.org/10.1111/jam.13128

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Improved proteomic analysis following trichloroacetic acid extraction of Bacillus anthracis spore proteins

Autor: Janine R. Hutchison, David S. Wunschel, Brooke L. Deatherage Kaiser, Karen L. Wahl, Michael A. Sydor, Marvin G. Warner

Publikováno v: Journal of Microbiological Methods. 118:18-24

Proteomic analysis of bacterial samples provides valuable information about cellular responses and functions under different environmental pressures. Analysis of cellular proteins is dependent upon efficient extraction from bacterial samples, which c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11df47921e7d135a9ad673c901f222a8
https://doi.org/10.1016/j.mimet.2015.08.008

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Identification of Salmonella Typhimurium Deubiquitinase SseL Substrates by Immunoaffinity Enrichment and Quantitative Proteomic Analysis

Autor: Jie Li, Rosa Di Leo, Tiago J. P. Sobreira, Michael A. Sydor, Gordon W. Slysz, Olena Onoprienko, Ernesto S. Nakayasu, Brooke L. Deatherage Kaiser, Charles Ansong, Tatiana Skarina, Joshua N. Adkins, Eric D. Cambronne, Daniel R. Humphrys, Richard D. Smith, Roslyn N. Brown, Alexei Savchenko, Ryan L. Sontag

Publikováno v: Journal of Proteome Research. 14:4029-4038

Ubiquitination is a key protein post-translational modification that regulates many important cellular pathways and whose levels are regulated by equilibrium between the activities of ubiquitin ligases and deubiquitinases. Here, we present a method t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50735a3947835e00d21e60e2212c28a4
https://doi.org/10.1021/acs.jproteome.5b00574

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Comparison of false-negative rates and limits of detection following macrofoam-swab sampling of Bacillus anthracis surrogates via Rapid Viability PCR and plate culture

Autor: Brett G. Amidan, Becky M. Hess, Janine R. Hutchison, B.L. Deatherage Kaiser, Gregory F. Piepel, Michael A. Sydor

Publikováno v: Journal of applied microbiology. 124(5)

Aims We evaluated the effects of Bacillus anthracis surrogates, low surface concentrations, surface materials and assay methods on false-negative rate (FNR) and limit of detection (LOD95 ) for recovering Bacillus spores using a macrofoam-swab samplin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab27cd070b4e36b05164112c62b34a2a
https://pubmed.ncbi.nlm.nih.gov/29356220

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Identification of Novel Host Interactors of Effectors Secreted by

Autor: Ryan L, Sontag, Ernesto S, Nakayasu, Roslyn N, Brown, George S, Niemann, Michael A, Sydor, Octavio, Sanchez, Charles, Ansong, Shao-Yeh, Lu, Hyungwon, Choi, Dylan, Valleau, Karl K, Weitz, Alexei, Savchenko, Eric D, Cambronne, Joshua N, Adkins

Publikováno v: mSystems

During infection, pathogenic bacteria face an adverse environment of factors driven by both cellular and humoral defense mechanisms. To help evade the immune response and ultimately proliferate inside the host, many bacteria evolved specialized secre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3f664b7a8501d43fdaffdba804bc61f3
https://pubmed.ncbi.nlm.nih.gov/27822540

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False Negative Rates of a Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates via Real-Time PCR

Autor: Gregory F. Piepel, Brett G. Amidan, Michael A. Sydor, Janine R. Hutchison, Brooke L. Deatherage Kaiser

Surface sampling for Bacillus anthracis spores has traditionally relied on detection via bacterial cultivation methods. Although effective, this approach does not provide the level of organism specificity that can be gained through molecular techniqu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df8db96174d260d8f8e066041ad62f38
https://doi.org/10.2172/1186982

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Recovery Efficiency, False Negative Rate, and Limit of Detection Performance of a Validated Macrofoam-Swab Sampling Method with Low Surface Concentrations of Two Bacillus anthracis Surrogates

Autor: Brett G. Amidan, Janine R. Hutchison, Brooke L. Deatherage Kaiser, Michael A. Sydor, Gregory F. Piepel, Christopher A. Barrett

The performance of a macrofoam-swab sampling method was evaluated using Bacillus anthracis Sterne (BAS) and Bacillus atrophaeus Nakamura (BG) spores applied at nine low target amounts (2-500 spores) to positive-control plates and test coupons (2 in.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::016ea539ce334f140100344bf0a0cf92
https://doi.org/10.2172/1179143

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Discerning Viable from Nonviable Yersinia Pestis pgm- and Bacillus Anthracis Sterne using Propidium Monoazide in the Presence of White Powders

Autor: Becky M. Hess, Cynthia J. Bruckner-Lea, Brooke L. Deatherage Kaiser, Janine R. Hutchison, David S. Wunschel, Michael A. Sydor

Publikováno v: Journal of Bioterrorism & Biodefense.

Purpose of the study To develop and optimize an assay to determine viability status of Bacillus anthracis Sterne and Yersinia pestis pgm- strains in the presence of white powders by coupling propidium monoazide (PMA) treatment with real-time PCR (qPC

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cd6338962b6cb6886874f82b0c761781
https://doi.org/10.4172/2157-2526.1000138

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A method to determine lysine acetylation stoichiometries

Autor: Matthew E. Monroe, Karl K. Weitz, Ronald J. Moore, Vladislav A. Petyuk, Charles Ansong, Si Wu, Joshua N. Adkins, Richard D. Smith, Michael A. Sydor, Ljiljiana Pasa-Tolic, Kim K. Hixson, Jong-Seo Kim, Ernesto S. Nakayasu, Anil K. Shukla, Wei-Jun Qian

Publikováno v: International Journal of Proteomics

Lysine acetylation is a common protein posttranslational modification that regulates a variety of biological processes. A major bottleneck to fully understanding the functional aspects of lysine acetylation is the difficulty in measuring the proporti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18f3219766955808ef057e47c2dee75f
https://pubmed.ncbi.nlm.nih.gov/25143833

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