Výsledky vyhledávání - "Michael A. Kosinski"

Proteolytic response to the expression of an abnormal beta-galactosidase in Escherichia coli

Autor: Ursula Rinas, Michael J. Kosinski, James E. Bailey

Publikováno v: Applied microbiology and biotechnology. 37(3)

Because induction of proteolytic activity and stress-response proteins can significantly affect expression levels in recombinant Escherichia coli, the influence of low-level expression of a mutant beta-galactosidase was investigated. A single copy of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c984c7cdb84e49a2d786f19c97e77264
https://pubmed.ncbi.nlm.nih.gov/1368906

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Structural characteristics of an abnormal protein influencing its proteolytic susceptibility

Autor: James E. Bailey, Michael J. Kosinski

Publikováno v: Journal of biotechnology. 23(2)

Structural properties of two similar beta-galactosidase fragments were investigated to determine how they influence the fragments' degradation rate in Escherichia coli. Both fragments resulting from a C-terminal nonsense mutation in lacZ, the CSH11 p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2b3f5e851fedd7d89c7957bb0cf0a26
https://pubmed.ncbi.nlm.nih.gov/1368059

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Isopropyl-?-d-thiogalactopyranoside influences the metabolism of Escherichia coli

Autor: Michael J. Kosinski, James E. Bailey, Ursula Rinas

Publikováno v: Applied Microbiology and Biotechnology. 36

Addition of isopropyl β-D-thiogalactopyranoside (IPTG) to a strain of Escherichia coli with one lac promoter in its chromosome causes reduction in synthesis rate for a set of protiens. One of these proteins, designated H35, is a prominent cellular p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f6b57f09a1bead9f719fddf1c6feb336
https://doi.org/10.1007/bf00172194

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