Dimerization deficiency of enigmatic retinitis pigmentosa-linked rhodopsin mutants

Autor: Birgit Ploier, Lydia N. Caro, Takefumi Morizumi, Kalpana Pandey, Jillian N. Pearring, Michael A. Goren, Silvia C. Finnemann, Johannes Graumann, Vadim Y. Arshavsky, Jeremy S. Dittman, Oliver P. Ernst, Anant K. Menon

Publikováno v: Nature Communications, Vol 7, Iss 1, Pp 1-11 (2016)

Retinitis pigmentosa is often caused by mutations that affect the activity or transport of rhodopsin, but some mutations cause disease even though an apparently functional protein is produced. Here the authors show that three such enigmatic mutants r

Externí odkaz: https://doaj.org/article/94c82e6657484b44baee46822dc7c91b

Light-independent phospholipid scramblase activity of bacteriorhodopsin from Halobacterium salinarum

Autor: Alice Verchère, Birgit Ploier, George Khelashvili, Takefumi Morizumi, Oliver P. Ernst, Wei-Lin Ou, Michael A. Goren, Peter Bütikofer, Anant K. Menon

Publikováno v: Verchère, Alice; Ou, Wei-Lin; Ploier, Birgit; Morizumi, Takefumi; Goren, Michael A; Bütikofer, Peter; Ernst, Oliver P; Khelashvili, George; Menon, Anant K (2017). Light-independent phospholipid scramblase activity of bacteriorhodopsin from Halobacterium salinarum. Scientific Reports, 7(1), p. 9522. Nature Publishing Group 10.1038/s41598-017-09835-5
Scientific Reports
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)

The retinylidene protein bacteriorhodopsin (BR) is a heptahelical light-dependent proton pump found in the purple membrane of the archaeon Halobacterium salinarum. We now show that when reconstituted into large unilamellar vesicles, purified BR trime

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea5e32f3b9a36f2ad44360929a3a9e09
https://boris.unibe.ch/110068/1/Light-independent.pdf

Cell-free Synthesis and Functional Characterization of Sphingolipid Synthases from Parasitic Trypanosomatid Protozoa

Autor: John Turk, Fong-Fu Hsu, James D. Bangs, Elitza S. Sevova, Kevin J. Schwartz, Michael A. Goren, Brian G. Fox

Publikováno v: Journal of Biological Chemistry. 285:20580-20587

The Trypanosoma brucei genome has four highly similar genes encoding sphingolipid synthases (TbSLS1-4). TbSLSs are polytopic membrane proteins that are essential for viability of the pathogenic bloodstream stage of this human protozoan parasite and,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a1bdab113f9a79b8e280c4c31ce5b14b
https://doi.org/10.1074/jbc.m110.127662

Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex

Autor: Brian G. Fox, Michael A. Goren

Publikováno v: Protein Expression and Purification. 62:171-178

A wheat germ cell-free extract was used to perform in vitro translation of human stearoyl-CoA desaturase in the presence of unilamelar liposomes, and near complete transfer of the expressed integral membrane protein into the liposome was observed. Mo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::158b844e4296833a6abe55cc2305d7bb
https://doi.org/10.1016/j.pep.2008.08.002

Constitutive phospholipid scramblase activity of a G protein-coupled receptor

Autor: Oliver P. Ernst, Indu Menon, Vadim Cherezov, Anant K. Menon, Takefumi Morizumi, Raymond C. Stevens, Jeremy S. Dittman, Michael A. Goren, Jeremiah S. Joseph

Publikováno v: Nature communications

Opsin, the rhodopsin apoprotein, was recently shown to be an ATP-independent flippase (or scramblase) that equilibrates phospholipids across photoreceptor disc membranes in mammalian retina, a process required for disc homoeostasis. Here we show that

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae4e0b7c00526a9a6d266462f387a025
https://doi.org/10.1038/ncomms6115