Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Michael Dauner"'
Autor:
Michael Dauner
Publikováno v:
Current Opinion in Biotechnology. 21:55-62
Fluxes and metabolites are the functional manifestations of a living cell. Metabolic flux analysis evolved as a powerful means for systems biology to quantitatively analyze intracellular flux distributions. With the integration of data from tracer ex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e012f573215176401fd5a943c6ad86d2
https://doi.org/10.1016/j.copbio.2010.01.014
https://doi.org/10.1016/j.copbio.2010.01.014
Publikováno v:
Biotechnology and Bioengineering. 101:135-152
Up to now cell-culture based vaccine production processes only reach low productivities. The reasons are: (i) slow cell growth and (ii) low cell concentrations. To address these shortcomings, a quantitative analysis of the process conditions, especia
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afcacb8d50a7e545fc502a044c267d6a
https://doi.org/10.1002/bit.21873
https://doi.org/10.1002/bit.21873
Publikováno v:
Biotechnology Progress. 24:311-320
In mammalian cell cultures, ammonia that is released into the medium as a result of glutamine metabolism and lactate that is excreted due to incomplete glucose oxidation are both known to essentially inhibit the growth of cells. For some cell lines,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90df242ad6b893ae9df347604cded939
https://doi.org/10.1021/bp0702673
https://doi.org/10.1021/bp0702673
Publikováno v:
Biotechnology and Bioengineering. 85:259-268
13C metabolic flux analysis (MFA) is based on carbon-labeling experiments where a specifically (13)C labeled substrate is fed. The labeled carbon atoms distribute over the metabolic network and the label enrichment of certain metabolic pools is measu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90c4d7bfb95afb7662fb3957e37165d1
https://doi.org/10.1002/bit.10909
https://doi.org/10.1002/bit.10909
Publikováno v:
Journal of Bacteriology. 183:7308-7317
The energetic efficiency of microbial growth is significantly reduced in cultures growing under glucose excess compared to cultures growing under glucose limitation, but the magnitude to which different energy-dissipating processes contribute to the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b02962ca3c4fe46488a7b929c9975044
https://doi.org/10.1128/jb.183.24.7308-7317.2001
https://doi.org/10.1128/jb.183.24.7308-7317.2001
Autor:
Michael Dauner
Publikováno v:
Engineering Complex Phenotypes in Industrial Strains
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b142ba9eecfd04f4239da1d81b794e14
https://doi.org/10.1002/9781118433034.ch2
https://doi.org/10.1002/9781118433034.ch2
Publikováno v:
Biotechnology progress. 22(5)
Metabolome analysis, the analysis of large sets of intracellular metabolites, has become an important systems analysis method in biotechnological and pharmaceutical research. In metabolic engineering, the integration of metabolome data with fluxome a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd49456edddb74bd335cac9ac03d1bb9
https://pubmed.ncbi.nlm.nih.gov/17022684
https://pubmed.ncbi.nlm.nih.gov/17022684
Autor:
Kurt Wüthrich, Michael Dauner, Hans-Peter Hohmann, Uwe Sauer, James E. Bailey, Thomas Szyperski, Marco Sonderegger, Michel Hochuli
Publikováno v:
Applied and environmental microbiology. 68(4)
Metabolic responses to cofeeding of different carbon substrates in carbon-limited chemostat cultures were investigated with riboflavin-producing Bacillus subtilis . Relative to the carbon content (or energy content) of the substrates, the biomass yie
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e565ebdf3bc3324af1281ddcf45e21fe
https://pubmed.ncbi.nlm.nih.gov/11916694
https://pubmed.ncbi.nlm.nih.gov/11916694
Autor:
Aaron Ponti, Uwe Sauer, Jocelyne Fiaux, Kurt Wüthrich, Michel Hochuli, Thomas Szyperski, James E. Bailey, Marcel Emmerling, Michael Dauner
Publikováno v:
Journal of bacteriology. 184(1)
The intracellular carbon flux distribution in wild-type and pyruvate kinase-deficient Escherichia coli was estimated using biosynthetically directed fractional 13 C labeling experiments with [U- 13 C 6 ]glucose in glucose- or ammonia-limited chemosta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba7998573560e3707e9c9577345b9959
https://pubmed.ncbi.nlm.nih.gov/11741855
https://pubmed.ncbi.nlm.nih.gov/11741855
Publikováno v:
Biotechnology and bioengineering. 76(2)
Fluxes in central carbon metabolism of a genetically engineered, riboflavin-producing Bacillus subtilis strain were investigated in glucose-limited chemostat cultures at low (0.11 h(-1)) and high (0.44 h(-1)) dilution rates. Using a mixture of 10% [U
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48113cafe9bd5cf42806cc4ae2ca676f
https://pubmed.ncbi.nlm.nih.gov/11505384
https://pubmed.ncbi.nlm.nih.gov/11505384