Zobrazeno 1 - 10
of 165
pro vyhledávání: '"Michael, Costanzo"'
Autor:
Kevin Lin, Ya-Chu Chang, Maximilian Billmann, Henry N. Ward, Khoi Le, Arshia Z. Hassan, Urvi Bhojoo, Katherine Chan, Michael Costanzo, Jason Moffat, Charles Boone, Anja-Katrin Bielinsky, Chad L. Myers
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-17 (2024)
Abstract Current approaches to define chemical-genetic interactions (CGIs) in human cell lines are resource-intensive. We designed a scalable chemical-genetic screening platform by generating a DNA damage response (DDR)-focused custom sgRNA library t
Externí odkaz:
https://doaj.org/article/3b9ce5f7b8554685b2e0afb2fc4f3cce
Autor:
Sylvia Varland, Rui Duarte Silva, Ine Kjosås, Alexandra Faustino, Annelies Bogaert, Maximilian Billmann, Hadi Boukhatmi, Barbara Kellen, Michael Costanzo, Adrian Drazic, Camilla Osberg, Katherine Chan, Xiang Zhang, Amy Hin Yan Tong, Simonetta Andreazza, Juliette J. Lee, Lyudmila Nedyalkova, Matej Ušaj, Alexander J. Whitworth, Brenda J. Andrews, Jason Moffat, Chad L. Myers, Kris Gevaert, Charles Boone, Rui Gonçalo Martinho, Thomas Arnesen
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-27 (2023)
Abstract Most eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal a strong genetic dependency between a major N-termina
Externí odkaz:
https://doaj.org/article/d00c6a73922e4ae69223e5ddad630fd5
Autor:
Michelle M Conti, Julie M Ghizzoni, Ana Gil-Bona, Wen Wang, Michael Costanzo, Rui Li, Mackenzie J Flynn, Lihua Julie Zhu, Chad L Myers, Charles Boone, Brenda J Andrews, Jennifer A Benanti
Publikováno v:
PLoS Genetics, Vol 18, Iss 8, p e1010349 (2022)
A network of transcription factors (TFs) coordinates transcription with cell cycle events in eukaryotes. Most TFs in the network are phosphorylated by cyclin-dependent kinase (CDK), which limits their activities during the cell cycle. Here, we invest
Externí odkaz:
https://doaj.org/article/ee09442a30bf4dfe8cda0546781b70c7
Autor:
Jessica R. Eisenstatt, Lars Boeckmann, Wei-Chun Au, Valerie Garcia, Levi Bursch, Josefina Ocampo, Michael Costanzo, Michael Weinreich, Robert A. Sclafani, Anastasia Baryshnikova, Chad L. Myers, Charles Boone, David J. Clark, Richard Baker, Munira A. Basrai
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 10, Iss 6, Pp 2057-2068 (2020)
The evolutionarily conserved centromeric histone H3 variant (Cse4 in budding yeast, CENP-A in humans) is essential for faithful chromosome segregation. Mislocalization of CENP-A to non-centromeric chromatin contributes to chromosomal instability (CIN
Externí odkaz:
https://doaj.org/article/18406182fbf848b19238234c13a0f3b1
Autor:
Gang Fang, Wen Wang, Vanja Paunic, Hamed Heydari, Michael Costanzo, Xiaoye Liu, Xiaotong Liu, Benjamin VanderSluis, Benjamin Oately, Michael Steinbach, Brian Van Ness, Eric E. Schadt, Nathan D. Pankratz, Charles Boone, Vipin Kumar, Chad L. Myers
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-18 (2019)
Genetic interactions may contribute to phenotypic traits but are challenging to decipher. Here, the authors develop BridGE, a computational approach for identifying pathways connected by genetic interactions from GWAS data.
Externí odkaz:
https://doaj.org/article/5820ce4d5cb54b95a38d7f821ba26707
Publikováno v:
Microbial Cell, Vol 6, Iss 6, Pp 286-294 (2019)
Ribonucleotide reductase (RNR) is an essential holoenzyme required for de novo synthesis of dNTPs. The Saccharomyces cerevisiae genome encodes for two catalytic subunits, Rnr1 and Rnr3. While Rnr1 is required for DNA replication and DNA damage repair
Externí odkaz:
https://doaj.org/article/ae93b009a3c14805bceb4ce2e6c9a37f
Autor:
Mahfuzur Rahman, Maximilian Billmann, Michael Costanzo, Michael Aregger, Amy H Y Tong, Katherine Chan, Henry N Ward, Kevin R Brown, Brenda J Andrews, Charles Boone, Jason Moffat, Chad L Myers
Publikováno v:
Molecular Systems Biology, Vol 17, Iss 5, Pp n/a-n/a (2021)
Abstract We present FLEX (Functional evaluation of experimental perturbations), a pipeline that leverages several functional annotation resources to establish reference standards for benchmarking human genome‐wide CRISPR screen data and methods for
Externí odkaz:
https://doaj.org/article/b77f0c4de34540ad85f7efa1a9046e4b
Autor:
Wei-Chun Au, Tianyi Zhang, Prashant K Mishra, Jessica R Eisenstatt, Robert L Walker, Josefina Ocampo, Anthony Dawson, Jack Warren, Michael Costanzo, Anastasia Baryshnikova, Karin Flick, David J Clark, Paul S Meltzer, Richard E Baker, Chad Myers, Charles Boone, Peter Kaiser, Munira A Basrai
Publikováno v:
PLoS Genetics, Vol 16, Iss 2, p e1008597 (2020)
Restricting the localization of the histone H3 variant CENP-A (Cse4 in yeast, CID in flies) to centromeres is essential for faithful chromosome segregation. Mislocalization of CENP-A leads to chromosomal instability (CIN) in yeast, fly and human cell
Externí odkaz:
https://doaj.org/article/4a4e4d4931964e2b9c6fa1e904c0dd0e
Autor:
Kamran Rizzolo, Jennifer Huen, Ashwani Kumar, Sadhna Phanse, James Vlasblom, Yoshito Kakihara, Hussein A. Zeineddine, Zoran Minic, Jamie Snider, Wen Wang, Carles Pons, Thiago V. Seraphim, Edgar Erik Boczek, Simon Alberti, Michael Costanzo, Chad L. Myers, Igor Stagljar, Charles Boone, Mohan Babu, Walid A. Houry
Publikováno v:
Cell Reports, Vol 20, Iss 11, Pp 2735-2748 (2017)
A comprehensive view of molecular chaperone function in the cell was obtained through a systematic global integrative network approach based on physical (protein-protein) and genetic (gene-gene or epistatic) interaction mapping. This allowed us to de
Externí odkaz:
https://doaj.org/article/f2af40acd84148669d6f31a51b5f7bd6
Autor:
Matej Usaj, Yizhao Tan, Wen Wang, Benjamin VanderSluis, Albert Zou, Chad L. Myers, Michael Costanzo, Brenda Andrews, Charles Boone
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 7, Iss 5, Pp 1539-1549 (2017)
Providing access to quantitative genomic data is key to ensure large-scale data validation and promote new discoveries. TheCellMap.org serves as a central repository for storing and analyzing quantitative genetic interaction data produced by genome-s
Externí odkaz:
https://doaj.org/article/8d6661a9770d4d72b591bcb9c7ff28de