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PCR assay to enhance global surveillance for SARS-CoV-2 variants of concern

Autor: Richard A. Neher, Jaqueline Goes de Jesus, Ngs-Sa, Mary E. Petrone, Ingra Morales Claro, Ester Cerdeira Sabino, Randy Downing, Ugochukwu J. Anyaneji, Tulio de Oliveira, Giulia M. Ferreira, Chen Liu, Megan Nash, Isabel M. Ott, Houriiyah Tegally, Emma B. Hodcroft, Steven Murphy, Tara Alpert, Chantal B.F. Vogels, Jianhui Wang, Joseph R. Fauver, Gaurav Khullar, Marie L. Landry, Caleb Neal, Nuno R. Faria, Mallery I. Breban, Nathan D. Grubaugh, Jafar Razeq, Christopher E. Mason, Lavanya Singh, Myuki Ae Crispim, Matthew MacKay, Metti Jessica, Anthony Muyombwe, Eva Laszlo, Joel T. Dudley, Pei Hui, Anne E. Watkins

Publikováno v: PLoS Biology

With the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants that may increase transmissibility and/or cause escape from immune responses, there is an urgent need for the targeted surveillance of circulating lineages. I

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20e7156a9b870f15e233c18bc5948414
https://pubmed.ncbi.nlm.nih.gov/33961632

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Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2.

Autor: Vogels CBF; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Breban MI; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Ott IM; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Alpert T; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Petrone ME; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Watkins AE; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Kalinich CC; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Earnest R; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Rothman JE; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Goes de Jesus J; Departamento de Molestias Infecciosas e Parasitarias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Morales Claro I; Departamento de Molestias Infecciosas e Parasitarias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Magalhães Ferreira G; Departamento de Molestias Infecciosas e Parasitarias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; Laboratório de Virologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil., Crispim MAE; Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas, Manaus, Brazil., Singh L; KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine & Medical Sciences, University of KwaZulu-Natal, Durban, South Africa., Tegally H; KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine & Medical Sciences, University of KwaZulu-Natal, Durban, South Africa., Anyaneji UJ; KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine & Medical Sciences, University of KwaZulu-Natal, Durban, South Africa., Hodcroft EB; Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland., Mason CE; Tempus Labs, Chicago, Illinois, United States of America., Khullar G; Tempus Labs, Chicago, Illinois, United States of America., Metti J; Tempus Labs, Chicago, Illinois, United States of America., Dudley JT; Tempus Labs, Chicago, Illinois, United States of America., MacKay MJ; Tempus Labs, Chicago, Illinois, United States of America., Nash M; Tempus Labs, Chicago, Illinois, United States of America., Wang J; Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, United States of America., Liu C; Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, United States of America., Hui P; Department of Pathology, Yale University School of Medicine, New Haven, Connecticut, United States of America., Murphy S; Murphy Medical Associates, Greenwich, Connecticut, United States of America., Neal C; Murphy Medical Associates, Greenwich, Connecticut, United States of America., Laszlo E; Murphy Medical Associates, Greenwich, Connecticut, United States of America., Landry ML; Departments of Laboratory Medicine and Medicine, Yale School of Medicine, New Haven, Connecticut, United States of America., Muyombwe A; Connecticut State Department of Public Health, Rocky Hill, Connecticut, United States of America., Downing R; Connecticut State Department of Public Health, Rocky Hill, Connecticut, United States of America., Razeq J; Connecticut State Department of Public Health, Rocky Hill, Connecticut, United States of America., de Oliveira T; KwaZulu-Natal Research Innovation and Sequencing Platform (KRISP), School of Laboratory Medicine & Medical Sciences, University of KwaZulu-Natal, Durban, South Africa., Faria NR; Departamento de Molestias Infecciosas e Parasitarias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; MRC Centre for Global Infectious Disease Analysis, J-IDEA, Imperial College London, London, United Kingdom.; Department of Zoology, University of Oxford, Oxford, United Kingdom., Sabino EC; Departamento de Molestias Infecciosas e Parasitarias and Instituto de Medicina Tropical da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Neher RA; Biozentrum, University of Basel, Basel, Switzerland.; Swiss Institute of Bioinformatics, Lausanne, Switzerland., Fauver JR; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America., Grubaugh ND; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, Connecticut, United States of America.; Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, United States of America.

Publikováno v: PLoS biology [PLoS Biol] 2021 May 07; Vol. 19 (5), pp. e3001236. Date of Electronic Publication: 2021 May 07 (Print Publication: 2021).

Early introductions and community transmission of SARS-CoV-2 variant B.1.1.7 in the United States.

Autor: Alpert T; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Brito AF; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Lasek-Nesselquist E; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.; Department of Biomedical Sciences, University at Albany, SUNY, Albany, NY 12222, USA., Rothman J; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Valesano AL; Department of Internal Medicine, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA., MacKay MJ; Tempus Labs, Chicago, IL 60654, USA., Petrone ME; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Breban MI; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Watkins AE; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Vogels CBF; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Kalinich CC; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Dellicour S; Spatial Epidemiology Lab (SpELL), Université Libre de Bruxelles, Bruxelles, Belgium.; Laboratory of Clinical and Epidemiological Virology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium., Russell A; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA., Kelly JP; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA., Shudt M; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.; Department of Biomedical Sciences, University at Albany, SUNY, Albany, NY 12222, USA., Plitnick J; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.; Department of Biomedical Sciences, University at Albany, SUNY, Albany, NY 12222, USA., Schneider E; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.; Department of Biomedical Sciences, University at Albany, SUNY, Albany, NY 12222, USA., Fitzsimmons WJ; Department of Internal Medicine, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA., Khullar G; Tempus Labs, Chicago, IL 60654, USA., Metti J; Tempus Labs, Chicago, IL 60654, USA., Dudley JT; Tempus Labs, Chicago, IL 60654, USA., Nash M; Tempus Labs, Chicago, IL 60654, USA., Beaubier N; Tempus Labs, Chicago, IL 60654, USA., Wang J; Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA., Liu C; Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA., Hui P; Department of Pathology, Yale University School of Medicine, New Haven, CT 06510, USA., Muyombwe A; Connecticut State Department of Public Health, Rocky Hill, CT 06067, USA., Downing R; Connecticut State Department of Public Health, Rocky Hill, CT 06067, USA., Razeq J; Connecticut State Department of Public Health, Rocky Hill, CT 06067, USA., Bart SM; Connecticut State Department of Public Health, Rocky Hill, CT 06067, USA.; Centers for Disease Control and Prevention, Atlanta, GA 30329, USA., Grills A; Centers for Disease Control and Prevention, Atlanta, GA 30329, USA., Morrison SM; Centers for Disease Control and Prevention, Atlanta, GA 30329, USA., Murphy S; Murphy Medical Associates, Greenwich, CT 06830, USA., Neal C; Murphy Medical Associates, Greenwich, CT 06830, USA., Laszlo E; Murphy Medical Associates, Greenwich, CT 06830, USA., Rennert H; Department of Pathology and Laboratory Medicine, New York Presbyterian Hospital-Weill Cornell Medicine, New York, NY 10021, USA., Cushing M; Department of Pathology and Laboratory Medicine, New York Presbyterian Hospital-Weill Cornell Medicine, New York, NY 10021, USA., Westblade L; Department of Pathology and Laboratory Medicine, New York Presbyterian Hospital-Weill Cornell Medicine, New York, NY 10021, USA., Velu P; Department of Pathology and Laboratory Medicine, New York Presbyterian Hospital-Weill Cornell Medicine, New York, NY 10021, USA., Craney A; Department of Pathology and Laboratory Medicine, New York Presbyterian Hospital-Weill Cornell Medicine, New York, NY 10021, USA., Fauntleroy KA; Department of Pathology and Laboratory Medicine, New York Presbyterian Hospital-Weill Cornell Medicine, New York, NY 10021, USA., Peaper DR; Departments of Laboratory Medicine and Medicine, Yale School of Medicine, New Haven, CT 06510, USA., Landry ML; Departments of Laboratory Medicine and Medicine, Yale School of Medicine, New Haven, CT 06510, USA., Cook PW; Centers for Disease Control and Prevention, Atlanta, GA 30329, USA., Fauver JR; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA., Mason CE; Tempus Labs, Chicago, IL 60654, USA.; Department of Pathology and Laboratory Medicine, New York Presbyterian Hospital-Weill Cornell Medicine, New York, NY 10021, USA., Lauring AS; Department of Internal Medicine, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA., George KS; Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.; Department of Biomedical Sciences, University at Albany, SUNY, Albany, NY 12222, USA., MacCannell DR; Centers for Disease Control and Prevention, Atlanta, GA 30329, USA., Grubaugh ND; Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA.; Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06510, USA.

Publikováno v: MedRxiv : the preprint server for health sciences [medRxiv] 2021 Mar 11. Date of Electronic Publication: 2021 Mar 11.