Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Meredith C. Gould"'
Maturation and fertilization inLottia giganteaoocytes: Intracellular pH, Ca2+, and electrophysiology
Publikováno v:
Journal of Experimental Zoology. 290:411-420
Intracellular pH and Ca(2+) were measured with BCECF- and Calcium Green-dextran during maturation and fertilization of oocytes of the limpet Lottia gigantea. Maturation of oocytes from prophase to metaphase I of meiosis was induced in seawater adjust
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7df3ef4adf291c24e2e1af4671f32aec
https://doi.org/10.1002/jez.1082
https://doi.org/10.1002/jez.1082
Autor:
Meredith C. Gould, José Luis Stephano
Publikováno v:
Invertebrate Reproduction & Development. 30:17-22
Summary This article reviews current knowledge about sperm; egg interactions and the initiation of development in Urechis caupo with emphasis on molecular mechanisms. Some important questions that remain to be answered are also indicated.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::643cbe19f03f046dff796120ba21cc19
https://doi.org/10.1080/07924259.1996.9672527
https://doi.org/10.1080/07924259.1996.9672527
Autor:
Meredith C. Gould, José Luis Stephano, Lourdes Aidée Solano‐Estrada, Luis Fernando Plenge‐Tellechea
Publikováno v:
Journal of Experimental Zoology. 272:484-489
Microinjection through the tough vitelline coats of oocytes from three molluscan species (Haliotis rufescens, Norrisia norrisi, and Astrea undosa) was facilitated by the local appli- cation of sperm vitelline coat lysin with a micropipet. Extracts co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2544a23f49c505860f289d5efbbebc9b
https://doi.org/10.1002/jez.1402720610
https://doi.org/10.1002/jez.1402720610
Autor:
Meredith C. Gould, José Luis Stephano
Publikováno v:
Developmental Biology. 167(1):104-117
A peptide (P23) isolated from sperm acrosomal protein initiates development in eggs of the marine worm Urechis caupo (Echiura). However, in these earlier experiments the eggs were exposed continuously to P23 and did not cleave unless they were provid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f0712208f1900d63880c018fdaa3f94
Autor:
Meredith C. Gould, JoséLuis Stephano
Publikováno v:
Developmental Biology. 146:509-518
How sperm initiate egg development is being investigated with gametes of the marine worm Urechis. Sperm acrosomal protein, previously shown to activate eggs ( Gould et al., 1986, Dev. Biol. 117, 306–318 ; Gould and Stephano, 1987, Science 235, 1654
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cdcf291546f6bc76bb545c235494999f
https://doi.org/10.1016/0012-1606(91)90252-x
https://doi.org/10.1016/0012-1606(91)90252-x
Publikováno v:
Biology of the cell. 95(5)
Oocytes of Crassostrea gigas and Mytilus galloprovincialis are arrested in metaphase I when they are spawned and ready to be fertilized. To investigate the role of MAP kinase in maintaining metaphase I arrest, oocytes were exposed to the MEK inhibito
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ec00c0aa3b1b3995bd7a499ea42043a
https://pubmed.ncbi.nlm.nih.gov/12941525
https://pubmed.ncbi.nlm.nih.gov/12941525
Autor:
Meredith C. Gould, José Luis Stephano
Publikováno v:
Microscopy research and technique. 61(4)
In marine invertebrates, as in most other organisms, normal development requires that only one sperm nucleus joins with the egg nucleus at fertilization. The principal mechanisms employed are (1) prevention of sperm-egg plasma membrane fusion and (2)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52eab218e2975ec8ef5f79924a274568
https://pubmed.ncbi.nlm.nih.gov/12811743
https://pubmed.ncbi.nlm.nih.gov/12811743
Autor:
Meredith C. Gould, José Luis Stephano
Publikováno v:
Development, growthdifferentiation. 39(1)
A peptide (P23) isolated from sperm acrosomal protein initiates development in eggs of the marine worm Urechis caupo. We have shown previously that eggs exposed to P23 for > or = 3 min complete meiosis but fail to cleave. However, a brief (1.5-2 min)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aae2bcf8bf39019eeb487a003c227284
https://pubmed.ncbi.nlm.nih.gov/9079038
https://pubmed.ncbi.nlm.nih.gov/9079038
Autor:
José Luis Stephano, Meredith C. Gould
Publikováno v:
Developmental biology. 159(2)
Intracellular pH (pHi) was measured in Urechis caupo (Echiura) eggs during fertilization using the pH-sensitive dye BCECF [bis(carboxyethyl)carboxyfluorescein] and fluorescence microscopy. When eggs were inseminated at pH 8, pHi began to rise 22-36 s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d31fa3bda6fa37eaf381f05a105a857
https://pubmed.ncbi.nlm.nih.gov/8405683
https://pubmed.ncbi.nlm.nih.gov/8405683
Autor:
Meredith C. Gould, José Luis Stephano
Publikováno v:
Aquaculture. 73:295-307
Polyspermy was assayed by counting sperm pronuclei in orcein-stained eggs of Crassostrea gigas with ordinary light microscopy. When oocytes were inseminated within 15 min of their mechanical removal from the ovary, they became highly polyspermic, for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1ced6404439a759cc80794043cf29c9e
https://doi.org/10.1016/0044-8486(88)90063-4
https://doi.org/10.1016/0044-8486(88)90063-4