Proteomic and functional comparison between human induced and embryonic stem cells

Autor: Alejandro J Brenes, Eva Griesser, Linda V Sinclair, Lindsay Davidson, Alan R Prescott, Francois Singh, Elizabeth KJ Hogg, Carmen Espejo-Serrano, Hao Jiang, Harunori Yoshikawa, Melpomeni Platani, Jason R Swedlow, Greg M Findlay, Doreen A Cantrell, Angus I Lamond

Publikováno v: eLife, Vol 13 (2024)

Human induced pluripotent stem cells (hiPSCs) have great potential to be used as alternatives to embryonic stem cells (hESCs) in regenerative medicine and disease modelling. In this study, we characterise the proteomes of multiple hiPSC and hESC line

Externí odkaz: https://doaj.org/article/a4ed042a121f45aca7b7b1fad3c2840c

The Inner Centromere Protein (INCENP) Coil Is a Single α-Helix (SAH) Domain That Binds Directly to Microtubules and Is Important for Chromosome Passenger Complex (CPC) Localization and Function in Mitosis*

Autor: Michelle Peckham, Kumiko Samejima, Peter J. Knight, Giulia Vargiu, Melpomeni Platani, Hiromi Ogawa, Marcin Wolny, William C. Earnshaw

Publikováno v: The Journal of Biological Chemistry
Samejima, K, Platani, M, Wolny, M, Ogawa, H, Vargiu, G, Knight, P J, Peckham, M & Earnshaw, W C 2015, ' The inner centromere protein (INCENP) coil is a single α-helix (SAH) domain that binds directly to microtubules and is important for chromosome passenger complex (CPC) localization and function in mitosis ', Journal of Biological Chemistry, vol. 290, no. 35, pp. 21460-21472 . https://doi.org/10.1074/jbc.M115.645317

Background: INCENP is predicted to have a coiled coil domain. Results: The coil is actually a stable single α-helix (SAH) domain that is highly extendable and directly binds microtubules. Conclusion: This flexible dog leash may allow Aurora B to ass

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4c2af8434834f86da7d0ea844fb3490
http://europepmc.org/articles/PMC4571873

Two Interlinked Bistable Switches Govern Mitotic Control in Mammalian Cells

Autor: Scott Rata, Maria F. Suarez Peredo Rodriguez, Stephy Joseph, Nisha Peter, Fabio Echegaray Iturra, Fengwei Yang, Anotida Madzvamuse, Jan G. Ruppert, Kumiko Samejima, Melpomeni Platani, Monica Alvarez-Fernandez, Marcos Malumbres, William C. Earnshaw, Bela Novak, Helfrid Hochegger

Publikováno v: Current Biology

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=r3c4b2081b22::cf9dd4c16fd6a25c932a0c6d63a0708c

Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis

Autor: Kumiko, Samejima, Daniel G, Booth, Hiromi, Ogawa, James R, Paulson, Linfeng, Xie, Cara A, Watson, Melpomeni, Platani, Masato T, Kanemaki, William C, Earnshaw

Publikováno v: Journal of Cell Science

The requirement for condensin in chromosome formation in somatic cells remains unclear, as imperfectly condensed chromosomes do form in cells depleted of condensin by conventional methodologies. In order to dissect the roles of condensin at different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::130f72aa789e6c22c9747b252b7a6279
https://pubmed.ncbi.nlm.nih.gov/29361541

HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry

Autor: Jan G, Ruppert, Kumiko, Samejima, Melpomeni, Platani, Oscar, Molina, Hiroshi, Kimura, A Arockia, Jeyaprakash, Shinya, Ohta, William C, Earnshaw

Publikováno v: The EMBO Journal

The chromosomal passenger complex (CPC) is directed to centromeres during mitosis via binding to H3T3ph and Sgo1. Whether and how heterochromatin protein 1α (HP1α) influences CPC localisation and function during mitotic entry is less clear. Here, w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d7e22564f7ab2c202b78dfc49f89961e
https://pubmed.ncbi.nlm.nih.gov/29467217