Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol

Autor: Daan Vorselen, Melissa C. Piontek, Wouter H. Roos, Gijs J. L. Wuite

Publikováno v: Frontiers in Molecular Biosciences, Vol 7 (2020)

Both natural as well as artificial vesicles are of tremendous interest in biology and nanomedicine. Small vesicles (

Externí odkaz: https://doaj.org/article/2104cf73f43e49fe8f55914d1b407d9f

Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms

Autor: Raya Sorkin, Rashmi Voleti, Josep Rizo, Guy Brand, Emma Logtenberg, Melissa C. Piontek, Gijs J.L. Wuite, Wouter H. Roos, Margherita Marchetti, Alexander J. Groffen, Emma Kerklingh

Publikováno v: Sorkin, R, Marchetti, M, Logtenberg, E, Piontek, M C, Kerklingh, E, Brand, G, Voleti, R, Rizo, J, Roos, W H, Groffen, A J & Wuite, G J L 2020, ' Synaptotagmin-1 and Doc2b Exhibit Distinct Membrane-Remodeling Mechanisms ', Biophysical Journal, vol. 118, no. 3, pp. 643-656 . https://doi.org/10.1016/j.bpj.2019.12.021
Biophysical Journal, 118(3), 643-656. Biophysical Society
Biophysical Journal, 118(3), 643-656. CELL PRESS
Biophys J

Synaptotagmin-1 (Syt1) is a calcium sensor protein that is critical for neurotransmission and is therefore extensively studied. Here, we use pairs of optically trapped beads coated with SNARE-free synthetic membranes to investigate Syt1-induced membr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b9f406d103fb959991bea979af8e0a6
https://doi.org/10.1016/j.bpj.2019.12.021

Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol

Autor: Melissa C. Piontek, Daan Vorselen, Gijs J.L. Wuite, Wouter H. Roos

Publikováno v: Frontiers in Molecular Biosciences, 7(July):139, 1-14. Frontiers Media S.A.
Frontiers in Molecular Biosciences, 7:139. Frontiers Media S.A.
Frontiers in Molecular Biosciences, Vol 7 (2020)
Frontiers in Molecular Biosciences
Vorselen, D, Piontek, M C, Roos, W H & Wuite, G J L 2020, ' Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol ', Frontiers in Molecular Biosciences, vol. 7, no. July, 139, pp. 1-14 . https://doi.org/10.3389/fmolb.2020.00139

Both natural as well as artificial vesicles are of tremendous interest in biology and nanomedicine. Small vesicles (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::593dffd332f9c9f9dc8c430314db4863
https://doi.org/10.3389/fmolb.2020.00139

The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis

Autor: Susan M. van Dommelen, Raya Sorkin, Richard van Wijk, Marc Bierings, Birgitta Versluijs, Jürgen Schiller, Raymond M. Schiffelers, Brigitte A. van Oirschot, Sander T. Döpp, Daan Vorselen, Gijs J.L. Wuite, Melissa C. Piontek, Wouter H. Roos, Sander A.A. Kooijmans

Publikováno v: Nature Communications, 9(1):4960. Nature Publishing Group
Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Nature Communications
Nature Communications, 9(1). Nature Publishing Group
Vorselen, D, van Dommelen, S M, Sorkin, R, Piontek, M C, Schiller, J, Döpp, S T, Kooijmans, S A A, van Oirschot, B A, Versluijs, B A, Bierings, M B, van Wijk, R, Schiffelers, R M, Wuite, G J L & Roos, W H 2018, ' The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis ', Nature Communications, vol. 9, 4960, pp. 1-9 . https://doi.org/10.1038/s41467-018-07445-x
Nature Communications, 9:4960, 1-9. Nature Publishing Group

Extracellular vesicles (EVs) are widely studied regarding their role in cell-to-cell communication and disease, as well as for applications as biomarkers or drug delivery vehicles. EVs contain membrane and intraluminal proteins, affecting their struc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5fe7a80da8f3c96a37e3ede779e4c0b3
http://www.scopus.com/inward/record.url?scp=85057079300&partnerID=8YFLogxK

Atomic Force Microscopy: An Introduction

Autor: Melissa C, Piontek, Wouter H, Roos

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1665

Imaging of nano-sized particles and sample features is crucial in a variety of research fields. For instance in biological sciences, where it is paramount to investigate structures at the single particle level. Often two-dimensional images are not su

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::470a61799fbec311460d628c23a15d67
https://pubmed.ncbi.nlm.nih.gov/28940073